α-tocopherol reduces lipopolysaccharide-induced peroxide radical formation and interleukin-6 secretion in primary murine microglia and in brain

Jonathan P. Godbout; Brian M. Berg; Keith W. Kelley; Rodney W. Johnson

doi:10.1016/j.jneuroim.2003.12.017

α-tocopherol reduces lipopolysaccharide-induced peroxide radical formation and interleukin-6 secretion in primary murine microglia and in brain

Jonathan P. Godbout, Brian M. Berg, Keith W. Kelley, Rodney W. Johnson

Research output: Contribution to journal › Article › peer-review

Abstract

The purpose of this study was to determine if α-tocopherol - a reactive oxygen species (ROS) scavenging agent - inhibits LPS-induced oxidative stress and interleukin-6 (IL-6) production in murine microglia and brain. LPS increased intracellular peroxides and IL-6 in a dose-dependent fashion in primary microglia. The LPS-induced increase in ROS and IL-6 was reduced by pretreatment of α-tocopherol. Intraperitoneal injection (i.p.) of LPS induced lipid peroxidation and IL-6 in brain. Importantly, three daily injections (20 mg) of α-tocopherol increased brain α-tocopherol and decreased LPS-induced lipid peroxidation and IL-6 in brain. Taken together, these data indicate that α-tocopherol can mitigate inflammatory cytokine production in the brain.

Original language	English (US)
Pages (from-to)	101-109
Number of pages	9
Journal	Journal of Neuroimmunology
Volume	149
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2003.12.017
State	Published - Apr 2004

Keywords

Brain
Cytokines
Lipopolysaccharide
Mice
Reactive oxygen species
α-Tocopherol

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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@article{fe795920547b4b379f09efa466d15abb,

title = "α-tocopherol reduces lipopolysaccharide-induced peroxide radical formation and interleukin-6 secretion in primary murine microglia and in brain",

abstract = "The purpose of this study was to determine if α-tocopherol - a reactive oxygen species (ROS) scavenging agent - inhibits LPS-induced oxidative stress and interleukin-6 (IL-6) production in murine microglia and brain. LPS increased intracellular peroxides and IL-6 in a dose-dependent fashion in primary microglia. The LPS-induced increase in ROS and IL-6 was reduced by pretreatment of α-tocopherol. Intraperitoneal injection (i.p.) of LPS induced lipid peroxidation and IL-6 in brain. Importantly, three daily injections (20 mg) of α-tocopherol increased brain α-tocopherol and decreased LPS-induced lipid peroxidation and IL-6 in brain. Taken together, these data indicate that α-tocopherol can mitigate inflammatory cytokine production in the brain.",

keywords = "Brain, Cytokines, Lipopolysaccharide, Mice, Reactive oxygen species, α-Tocopherol",

author = "Godbout, {Jonathan P.} and Berg, {Brian M.} and Kelley, {Keith W.} and Johnson, {Rodney W.}",

note = "Funding Information: This research was supported by NIH grants AG16710 and MH069148. J.P.G. is supported by a Ruth L. Kirchstein NRSA Postdoctoral Fellowship.",

year = "2004",

month = apr,

doi = "10.1016/j.jneuroim.2003.12.017",

language = "English (US)",

volume = "149",

pages = "101--109",

journal = "Advances in Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier",

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TY - JOUR

T1 - α-tocopherol reduces lipopolysaccharide-induced peroxide radical formation and interleukin-6 secretion in primary murine microglia and in brain

AU - Godbout, Jonathan P.

AU - Berg, Brian M.

AU - Kelley, Keith W.

AU - Johnson, Rodney W.

N1 - Funding Information: This research was supported by NIH grants AG16710 and MH069148. J.P.G. is supported by a Ruth L. Kirchstein NRSA Postdoctoral Fellowship.

PY - 2004/4

Y1 - 2004/4

N2 - The purpose of this study was to determine if α-tocopherol - a reactive oxygen species (ROS) scavenging agent - inhibits LPS-induced oxidative stress and interleukin-6 (IL-6) production in murine microglia and brain. LPS increased intracellular peroxides and IL-6 in a dose-dependent fashion in primary microglia. The LPS-induced increase in ROS and IL-6 was reduced by pretreatment of α-tocopherol. Intraperitoneal injection (i.p.) of LPS induced lipid peroxidation and IL-6 in brain. Importantly, three daily injections (20 mg) of α-tocopherol increased brain α-tocopherol and decreased LPS-induced lipid peroxidation and IL-6 in brain. Taken together, these data indicate that α-tocopherol can mitigate inflammatory cytokine production in the brain.

AB - The purpose of this study was to determine if α-tocopherol - a reactive oxygen species (ROS) scavenging agent - inhibits LPS-induced oxidative stress and interleukin-6 (IL-6) production in murine microglia and brain. LPS increased intracellular peroxides and IL-6 in a dose-dependent fashion in primary microglia. The LPS-induced increase in ROS and IL-6 was reduced by pretreatment of α-tocopherol. Intraperitoneal injection (i.p.) of LPS induced lipid peroxidation and IL-6 in brain. Importantly, three daily injections (20 mg) of α-tocopherol increased brain α-tocopherol and decreased LPS-induced lipid peroxidation and IL-6 in brain. Taken together, these data indicate that α-tocopherol can mitigate inflammatory cytokine production in the brain.

KW - Brain

KW - Cytokines

KW - Lipopolysaccharide

KW - Mice

KW - Reactive oxygen species

KW - α-Tocopherol

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U2 - 10.1016/j.jneuroim.2003.12.017

DO - 10.1016/j.jneuroim.2003.12.017

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C2 - 15020070

AN - SCOPUS:1542710617

VL - 149

SP - 101

EP - 109

JO - Advances in Neuroimmunology

JF - Advances in Neuroimmunology

SN - 0165-5728

IS - 1-2

ER -

α-tocopherol reduces lipopolysaccharide-induced peroxide radical formation and interleukin-6 secretion in primary murine microglia and in brain

Abstract

Keywords

ASJC Scopus subject areas

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