α-tocopherol reduces lipopolysaccharide-induced peroxide radical formation and interleukin-6 secretion in primary murine microglia and in brain

Jonathan P. Godbout, Brian M. Berg, Keith W. Kelley, Rodney W. Johnson

Research output: Contribution to journalArticlepeer-review

Abstract

The purpose of this study was to determine if α-tocopherol - a reactive oxygen species (ROS) scavenging agent - inhibits LPS-induced oxidative stress and interleukin-6 (IL-6) production in murine microglia and brain. LPS increased intracellular peroxides and IL-6 in a dose-dependent fashion in primary microglia. The LPS-induced increase in ROS and IL-6 was reduced by pretreatment of α-tocopherol. Intraperitoneal injection (i.p.) of LPS induced lipid peroxidation and IL-6 in brain. Importantly, three daily injections (20 mg) of α-tocopherol increased brain α-tocopherol and decreased LPS-induced lipid peroxidation and IL-6 in brain. Taken together, these data indicate that α-tocopherol can mitigate inflammatory cytokine production in the brain.

Original languageEnglish (US)
Pages (from-to)101-109
Number of pages9
JournalJournal of Neuroimmunology
Volume149
Issue number1-2
DOIs
StatePublished - Apr 2004

Keywords

  • Brain
  • Cytokines
  • Lipopolysaccharide
  • Mice
  • Reactive oxygen species
  • α-Tocopherol

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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