Abstract
The findings presented here demonstrate the role of α-catenin in cadherin-based adhesion and mechanotransduction in different mechanical contexts. Bead-twisting measurements in conjunction with imaging, and the use of different cell lines and α-catenin mutants reveal that the acute local mechanical manipulation of cadherin bonds triggers vinculin and actin recruitment to cadherin adhesions in an actin- and α-catenin-dependent manner. The modest effect of α-catenin on the two-dimensional binding affinities of cell surface cadherins further suggests that forceactivated adhesion strengthening is due to enhanced cadherin-cytoskeletal interactions rather than to α-catenin-dependent affinity modulation. Complementary investigations of cadherin-based rigidity sensing also suggest that, although α-catenin alters traction force generation, it is not the sole regulator of cell contractility on compliant cadherin-coated substrata.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1779-1791 |
| Number of pages | 13 |
| Journal | Journal of cell science |
| Volume | 127 |
| Issue number | 8 |
| DOIs | |
| State | Published - 2014 |
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Keywords
- Adhesion
- Cadherin
- Mechanotransduction
- α-Catenin
ASJC Scopus subject areas
- Cell Biology
Cite this
α-Catenin cytomechanics - role in cadherin-dependent adhesion and mechanotransduction. / Barry, Adrienne K.; Tabdili, Hamid; Muhamed, Ismaeel; Wu, Jun; Shashikanth, Nitesh; Gomez, Guillermo A.; Yap, Alpha S.; Gottardi, Cara J.; De Rooij, Johan; Wang, Ning; Leckband, Deborah E.
In: Journal of cell science, Vol. 127, No. 8, 2014, p. 1779-1791.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - α-Catenin cytomechanics - role in cadherin-dependent adhesion and mechanotransduction
AU - Barry, Adrienne K.
AU - Tabdili, Hamid
AU - Muhamed, Ismaeel
AU - Wu, Jun
AU - Shashikanth, Nitesh
AU - Gomez, Guillermo A.
AU - Yap, Alpha S.
AU - Gottardi, Cara J.
AU - De Rooij, Johan
AU - Wang, Ning
AU - Leckband, Deborah E.
PY - 2014
Y1 - 2014
N2 - The findings presented here demonstrate the role of α-catenin in cadherin-based adhesion and mechanotransduction in different mechanical contexts. Bead-twisting measurements in conjunction with imaging, and the use of different cell lines and α-catenin mutants reveal that the acute local mechanical manipulation of cadherin bonds triggers vinculin and actin recruitment to cadherin adhesions in an actin- and α-catenin-dependent manner. The modest effect of α-catenin on the two-dimensional binding affinities of cell surface cadherins further suggests that forceactivated adhesion strengthening is due to enhanced cadherin-cytoskeletal interactions rather than to α-catenin-dependent affinity modulation. Complementary investigations of cadherin-based rigidity sensing also suggest that, although α-catenin alters traction force generation, it is not the sole regulator of cell contractility on compliant cadherin-coated substrata.
AB - The findings presented here demonstrate the role of α-catenin in cadherin-based adhesion and mechanotransduction in different mechanical contexts. Bead-twisting measurements in conjunction with imaging, and the use of different cell lines and α-catenin mutants reveal that the acute local mechanical manipulation of cadherin bonds triggers vinculin and actin recruitment to cadherin adhesions in an actin- and α-catenin-dependent manner. The modest effect of α-catenin on the two-dimensional binding affinities of cell surface cadherins further suggests that forceactivated adhesion strengthening is due to enhanced cadherin-cytoskeletal interactions rather than to α-catenin-dependent affinity modulation. Complementary investigations of cadherin-based rigidity sensing also suggest that, although α-catenin alters traction force generation, it is not the sole regulator of cell contractility on compliant cadherin-coated substrata.
KW - Adhesion
KW - Cadherin
KW - Mechanotransduction
KW - α-Catenin
UR - http://www.scopus.com/inward/record.url?scp=84898925056&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84898925056&partnerID=8YFLogxK
U2 - 10.1242/jcs.139014
DO - 10.1242/jcs.139014
M3 - Article
C2 - 24522187
AN - SCOPUS:84898925056
VL - 127
SP - 1779
EP - 1791
JO - Journal of Cell Science
JF - Journal of Cell Science
SN - 0021-9533
IS - 8
ER -