α-Catenin cytomechanics - role in cadherin-dependent adhesion and mechanotransduction

Adrienne K. Barry, Hamid Tabdili, Ismaeel Muhamed, Jun Wu, Nitesh Shashikanth, Guillermo A. Gomez, Alpha S. Yap, Cara J. Gottardi, Johan De Rooij, Ning Wang, Deborah E. Leckband

Research output: Contribution to journalArticle

Abstract

The findings presented here demonstrate the role of α-catenin in cadherin-based adhesion and mechanotransduction in different mechanical contexts. Bead-twisting measurements in conjunction with imaging, and the use of different cell lines and α-catenin mutants reveal that the acute local mechanical manipulation of cadherin bonds triggers vinculin and actin recruitment to cadherin adhesions in an actin- and α-catenin-dependent manner. The modest effect of α-catenin on the two-dimensional binding affinities of cell surface cadherins further suggests that forceactivated adhesion strengthening is due to enhanced cadherin-cytoskeletal interactions rather than to α-catenin-dependent affinity modulation. Complementary investigations of cadherin-based rigidity sensing also suggest that, although α-catenin alters traction force generation, it is not the sole regulator of cell contractility on compliant cadherin-coated substrata.

Original languageEnglish (US)
Pages (from-to)1779-1791
Number of pages13
JournalJournal of cell science
Volume127
Issue number8
DOIs
StatePublished - 2014

Fingerprint

Catenins
Cadherins
Actins
Vinculin
Traction
Cell Line

Keywords

  • Adhesion
  • Cadherin
  • Mechanotransduction
  • α-Catenin

ASJC Scopus subject areas

  • Cell Biology

Cite this

α-Catenin cytomechanics - role in cadherin-dependent adhesion and mechanotransduction. / Barry, Adrienne K.; Tabdili, Hamid; Muhamed, Ismaeel; Wu, Jun; Shashikanth, Nitesh; Gomez, Guillermo A.; Yap, Alpha S.; Gottardi, Cara J.; De Rooij, Johan; Wang, Ning; Leckband, Deborah E.

In: Journal of cell science, Vol. 127, No. 8, 2014, p. 1779-1791.

Research output: Contribution to journalArticle

Barry, AK, Tabdili, H, Muhamed, I, Wu, J, Shashikanth, N, Gomez, GA, Yap, AS, Gottardi, CJ, De Rooij, J, Wang, N & Leckband, DE 2014, 'α-Catenin cytomechanics - role in cadherin-dependent adhesion and mechanotransduction', Journal of cell science, vol. 127, no. 8, pp. 1779-1791. https://doi.org/10.1242/jcs.139014
Barry AK, Tabdili H, Muhamed I, Wu J, Shashikanth N, Gomez GA et al. α-Catenin cytomechanics - role in cadherin-dependent adhesion and mechanotransduction. Journal of cell science. 2014;127(8):1779-1791. https://doi.org/10.1242/jcs.139014
Barry, Adrienne K. ; Tabdili, Hamid ; Muhamed, Ismaeel ; Wu, Jun ; Shashikanth, Nitesh ; Gomez, Guillermo A. ; Yap, Alpha S. ; Gottardi, Cara J. ; De Rooij, Johan ; Wang, Ning ; Leckband, Deborah E. / α-Catenin cytomechanics - role in cadherin-dependent adhesion and mechanotransduction. In: Journal of cell science. 2014 ; Vol. 127, No. 8. pp. 1779-1791.
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