α -Amylase and α -Glucosidase Inhibitory Activities of Phenolic Extracts from Eucalyptus grandis × E. Urophylla Bark

Ping Jiang, Jia Xiong, Fei Wang, Mary H. Grace, Mary Ann Lila, Rui Xu

Research output: Contribution to journalArticle

Abstract

This study evaluated the inhibitory effects of different extracts and fractions from Eucalyptus. grandis × urophylla bark (EB) against α-glucosidase and α-amylase enzyme activities. The ethyl acetate extract (EB-E) showed the highest activity among others. Seven fractions were derived from EB-E; among them EB-E-7 showed the highest significant inhibition of both enzymes, with IC50 of 1.40±0.18 and 1.72±0.12 μg/mL, respectively. EB-E and its active fraction EB-E-7 showed highest contents of total phenolics: 178.79±4.68 and 920.4±5.46 mg GAEag-1, respectively. HPLC-MS analysis of EB-E-7 revealed the presence of ellagic acid, quercetin-glucuronide, quercetin-3-α-rhamnopyranoside, and ellagic acid rhamnoside as major compounds, together with smaller concentrations of myricetin-rhamnoside, isorhamnetin-hexoside, myricetin-3-α-arabinofuranoside, and isorhamnetin. Therefore, the phenolic compounds from Eucalyptus grandis × E. urograndis bark potently inhibited α-amylase and α-glucosidase activity, having potential in prevention of hyperglycemia.

Original languageEnglish (US)
Article number8516964
JournalJournal of Chemistry
Volume2017
DOIs
StatePublished - 2017

ASJC Scopus subject areas

  • Chemistry(all)

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