William Harold Witola

19972018
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Research Output 1997 2018

2018

In vitro activity of the interaction between taxifolin (dihydroquercetin) and pyrimethamine against Toxoplasma gondii

Abugri, D. A., Witola, W. H., Russell, A. E. & Troy, R. M. Jan 1 2018 In : Chemical Biology and Drug Design. 91, 1, p. 194-201 8 p.

Research output: Contribution to journalArticle

Pyrimethamine
Toxoplasma
Toxoplasmosis
Parasitic Diseases
Foodborne Diseases

Morpholino-mediated in vivo silencing of Cryptosporidium parvum lactate dehydrogenase decreases oocyst shedding and infectivity

Zhang, X., Kim, C. Y., Worthen, T. & Witola, W. H. Jul 1 2018 In : International Journal for Parasitology. 48, 8, p. 649-656 8 p.

Research output: Contribution to journalArticle

Cryptosporidium parvum
Morpholinos
Oocysts
L-Lactate Dehydrogenase
Gene Knockdown Techniques

Small GTPase immunity-associated proteins mediate resistance to Toxoplasma gondii infection in Lewis rat

Kim, C. Y., Zhang, X. & Witola, W. H. Apr 1 2018 In : Infection and Immunity. 86, 4, e00582-17

Research output: Contribution to journalArticle

Monomeric GTP-Binding Proteins
Toxoplasmosis
Immunity
Toxoplasma
GTP Phosphohydrolases
2017

Inherent oxidative stress in the Lewis rat is associated with resistance to toxoplasmosis

Witola, W. H., Kim, C. Y. & Zhang, X. Jan 1 2017 In : Infection and Immunity. 85, 10, e00289-17

Research output: Contribution to journalArticle

Toxoplasmosis
Oxidative Stress
Toxoplasma
Reactive Oxygen Species
Glutathione Transferase

In vitro antagonistic and indifferent activity of combination of 3-deoxyanthocyanidins against Toxoplasma gondii

Abugri, D. A., Witola, W. H. & Jaynes, J. M. Dec 1 2017 In : Parasitology Research. 116, 12, p. 3387-3400 14 p.

Research output: Contribution to journalArticle

Toxoplasma
Toxoplasma gondii
Parasites
Foreskin
parasites

Targeted gene knockdown validates the essential role of lactate dehydrogenase in Cryptosporidium parvum

Witola, W. H., Zhang, X. & Kim, C. Y. Nov 1 2017 In : International Journal for Parasitology. 47, 13, p. 867-874 8 p.

Research output: Contribution to journalArticle

Gene Knockdown Techniques
Cryptosporidium parvum
L-Lactate Dehydrogenase
Morpholinos
nitazoxanide
2016

In vitro activity of Sorghum bicolor extracts, 3-deoxyanthocyanidins, against Toxoplasma gondii

Abugri, D. A., Witola, W. H., Jaynes, J. M. & Toufic, N. May 1 2016 In : Experimental Parasitology. 164, p. 12-19 8 p.

Research output: Contribution to journalArticle

Sorghum
Toxoplasma
Antiparasitic Agents
Chloroform
Ethanol
Haemonchus
Anthelmintics
Larva
Antiparasitic Agents
Phosphorylcholine

Knockdown of phosphoethanolamine transmethylation enzymes decreases viability of Haemonchus contortus

Witola, W. H., Cooks-Fagbodun, S., Ordonez, A. R., Matthews, K., Abugri, D. A. & McHugh, M. Jun 15 2016 In : Veterinary parasitology. 223, p. 1-6 6 p.

Research output: Contribution to journalArticle

Haemonchus
Haemonchus contortus
Reverse Genetics
Morpholinos
Caenorhabditis elegans
2014

ALOX12 in human toxoplasmosis

Witola, W. H., Liu, S. R., Montpetit, A., Welti, R., Hypolite, M., Roth, M., Zhou, Y., Mui, E., Cesbron-Delauw, M. F., Fournie, G. J., Cavailles, P., Bisanz, C., Boyer, K., Withers, S., Gwendolyn Noble, A., Swisher, C. N., Heydemann, P. T., Rabiah, P., Muench, S. P. & McLeod, R. Jan 1 2014 In : Infection and Immunity. 82, 7, p. 2670-2679 10 p.

Research output: Contribution to journalArticle

Toxoplasmosis
Hydroxyeicosatetraenoic Acids
Arachidonic Acid
Genes
Parasites

Silencing of GRA10 protein expression inhibits Toxoplasma gondii intracellular growth and development

Witola, W. H., Bauman, B., McHugh, M. & Matthews, K. Jan 1 2014 In : Parasitology International. 63, 5, p. 651-658 8 p.

Research output: Contribution to journalArticle

Toxoplasma
Growth and Development
Vacuoles
Proteins
Parasites
2013

Isolation, cloning, and pathologic analysis of Trypanosoma evansi field isolates

Mekata, H., Konnai, S., Mingala, C. N., Abes, N. S., Gutierrez, C. A., Dargantes, A. P., Witola, W. H., Inoue, N., Onuma, M., Murata, S. & Ohashi, K. Apr 1 2013 In : Parasitology Research. 112, 4, p. 1513-1521 9 p.

Research output: Contribution to journalArticle

Trypanosoma
Trypanosoma evansi
Virulence
Organism Cloning
Philippines

Plasmodium falciparum phosphoethanolamine methyltransferase is essential for malaria transmission

Bobenchik, A. M., Witola, W. H., Augagneur, Y., Lochlainn, L. N., Garg, A., Pachikara, N., Choi, J. Y., Zhao, Y. O., Usmani-Brown, S., Lee, A., Adjalley, S. H., Samanta, S., Fidock, D. A., Voelker, D. R., Fikrig, E. & Mamoun, C. B. Nov 5 2013 In : Proceedings of the National Academy of Sciences of the United States of America. 110, 45, p. 18262-18267 6 p.

Research output: Contribution to journalArticle

Plasmodium falciparum
Malaria
Parasites
Culicidae
Plasmodium malariae
2012

Kinetics of regulatory dendritic cells in inflammatory responses during Trypanosoma evansi infection

Mekata, H., Konnai, S., Mingala, C. N., Abes, N. S., Gutierrez, C. A., Dargantes, A. P., Witola, W. H., Inoue, N., Onuma, M., Murata, S. & Ohashi, K. Jun 2012 In : Parasite Immunology. 34, 6, p. 318-329 12 p.

Research output: Contribution to journalArticle

Trypanosoma
Dendritic Cells
Infection
Wasting Syndrome
Chemokines

Molecular target validation, antimicrobial delivery, and potential treatment of Toxoplasma gondii infections

Lai, B. S., Witola, W. H., El Bissati, K., Zhou, Y., Mui, E., Fomovska, A. & McLeod, R. Aug 28 2012 In : Proceedings of the National Academy of Sciences of the United States of America. 109, 35, p. 14182-14187 6 p.

Research output: Contribution to journalArticle

Toxoplasmosis
Peptides
Toxoplasma
Tetrahydrofolate Dehydrogenase
Transcription Factor 3

Novel N-benzoyl-2-hydroxybenzamide disrupts unique parasite secretory pathway

Fomovska, A. , Huang, Q. , El Bissati, K. , Mui, E. J. , Witola, W. H. , Cheng, G. , Zhou, Y. , Sommerville, C. , Roberts, C. W. , Bettis, S. , Prigge, S. T. , Afanador, G. A. , Hickman, M. R. , Lee, P. J. , Leed, S. E. , Auschwitz, J. M. , Pieroni, M. , Stec, J. , Muench, S. P. , Rice, D. W. & 2 others Kozikowski, A. P. & McLeod, R. May 1 2012 In : Antimicrobial Agents and Chemotherapy. 56, 5, p. 2666-2682 17 p.

Research output: Contribution to journalArticle

Secretory Pathway
Toxoplasma
Parasites
Vacuoles
Proteins

Synthesis, biological evaluation, and structure-activity relationships of N -benzoyl-2-hydroxybenzamides as agents active against P. falciparum (K1 strain), trypanosomes, and leishmania

Stec, J., Huang, Q., Pieroni, M., Kaiser, M., Fomovska, A., Mui, E., Witola, W. H., Bettis, S., McLeod, R., Brun, R. & Kozikowski, A. P. Apr 12 2012 In : Journal of Medicinal Chemistry. 55, 7, p. 3088-3100 13 p.

Research output: Contribution to journalArticle

Trypanosomiasis
Leishmania
Toxoplasma
Structure-Activity Relationship
Plasmodium falciparum

Toxoplasma gondii HLA-B*0702-restricted GRA7 20-28 peptide with adjuvants and a universal helper T cell epitope elicits CD8 + T cells producing interferon-γ and reduces parasite burden in HLA-B*0702 mice

Cong, H., Mui, E. J., Witola, W. H., Sidney, J., Alexander, J., Sette, A., Maewal, A., El Bissati, K., Zhou, Y., Suzuki, Y., Lee, D., Woods, S., Sommerville, C., Henriquez, F. L., Roberts, C. W. & McLeod, R. Jan 1 2012 In : Human Immunology. 73, 1, p. 1-10 10 p.

Research output: Contribution to journalArticle

T-Lymphocyte Epitopes
Toxoplasma
Helper-Inducer T-Lymphocytes
Interferon-gamma
Parasites
2011

NALP1 influences susceptibility to human congenital toxoplasmosis, proinflammatory cytokine response, and fate of Toxoplasma gondii-infected monocytic cells

Witola, W. H., Mui, E., Hargrave, A., Liu, S., Hypolite, M., Montpetit, A., Cavailles, P., Bisanz, C., Cesbron-Delauw, M. F., Fournié, G. J. & McLeod, R. Feb 1 2011 In : Infection and Immunity. 79, 2, p. 756-766 11 p.

Research output: Contribution to journalArticle

Congenital Toxoplasmosis
Inflammasomes
Toxoplasma
Toxoplasmosis
Cytokines

Towards an immunosense vaccine to prevent toxoplasmosis: Protective Toxoplasma gondii epitopes restricted by HLA-A*0201

Cong, H., Mui, E. J., Witola, W. H., Sidney, J., Alexander, J., Sette, A., Maewal, A. & McLeod, R. Jan 17 2011 In : Vaccine. 29, 4, p. 754-762 9 p.

Research output: Contribution to journalArticle

toxoplasmosis
Toxoplasmosis
Toxoplasma
Toxoplasma gondii
epitopes
2010
Transgenic Mice
Immunization
Epitopes
T-Lymphocyte Epitopes
HLA-A Antigens

Identification of T. gondii epitopes, adjuvants, and host genetic factors that influence protection of mice and humans

Tan, T. G., Mui, E., Cong, H., Witola, W. H., Montpetit, A., Muench, S. P., Sidney, J., Alexander, J., Sette, A., Grigg, M. E., Maewal, A. & McLeod, R. May 21 2010 In : Vaccine. 28, 23, p. 3977-3989 13 p.

Research output: Contribution to journalArticle

epitopes
adjuvants
Epitopes
Aminopeptidases
aminopeptidases

P2X7 receptor-mediated killing of an intracellular parasite, Toxoplasma gondii, by human and murine macrophages

Lees, M. P., Fuller, S. J., McLeod, R., Boulter, N. R., Miller, C. M., Zakrzewski, A. M., Mui, E. J., Witola, W. H., Coyne, J. J., Hargrave, A. C., Jamieson, S. E., Blackwell, J. M., Wiley, J. S. & Smith, N. C. Jun 15 2010 In : Journal of Immunology. 184, 12, p. 7040-7046 7 p.

Research output: Contribution to journalArticle

Toxoplasma
Parasites
Macrophages
Single Nucleotide Polymorphism
Adenosine Triphosphate

T. gondii RP promoters & knockdown reveal molecular pathways associated with proliferation and cell-cycle arrest

Hutson, S. L., Mui, E., Kinsley, K., Witola, W. H., Behnke, M. S., El Bissati, K., Muench, S. P., Rohrman, B., Liu, S. R., Wollmann, R., Ogata, Y., Sarkeshik, A., Yates, J. R. & McLeod, R. Dec 3 2010 In : PLoS ONE. 5, 11, e14057

Research output: Contribution to journalArticle

Ribosomal Proteins
ribosomal proteins
Cell Cycle Checkpoints
Parasites
Cells

The malarial CDK Pfmrk and its effector PfMAT1 phosphorylate DNA replication proteins and co-localize in the nucleus

Jirage, D., Chen, Y., Caridha, D., O'Neil, M. T., Eyase, F., Witola, W. H., Mamoun, C. B. & Waters, N. C. Jul 1 2010 In : Molecular and Biochemical Parasitology. 172, 1, p. 9-18 10 p.

Research output: Contribution to journalArticle

Cyclin-Dependent Kinases
DNA Replication
Parasites
Proteins
Falciparum Malaria
2009

Development and application of a quantitative real-time PCR for the diagnosis of Surra in water buffaloes

Konnai, S., Mekata, H., Mingala, C. N., Abes, N. S., Gutierrez, C. A., Herrera, J. R. V., Dargantes, A. P., Witola, W. H., Cruz, L. C., Inoue, N., Onuma, M. & Ohashi, K. Jul 1 2009 In : Infection, Genetics and Evolution. 9, 4, p. 449-452 4 p.

Research output: Contribution to journalArticle

surra
Trypanosoma
Trypanosoma evansi
Buffaloes
Parasitemia

Expression and activity of glycogen synthase kinase during vitellogenesis and embryogenesis of Rhipicephalus (Boophilus) microplus

Logullo, C., Witola, W. H., Andrade, C., Abreu, L., Gomes, J., da Silva Vaz, I., Imamura, S., Konnai, S., Ohashi, K. & Onuma, M. May 12 2009 In : Veterinary parasitology. 161, 3-4, p. 261-269 9 p.

Research output: Contribution to journalArticle

Rhipicephalus
Vitellogenesis
Glycogen Synthase Kinases
Rhipicephalus microplus
vitellogenesis

Molecular detection of trypanosomes in cattle in South America and genetic diversity of Trypanosoma evansi based on expression-site-associated gene 6

Mekata, H., Konnai, S., Witola, W. H., Inoue, N., Onuma, M. & Ohashi, K. Dec 1 2009 In : Infection, Genetics and Evolution. 9, 6, p. 1301-1305 5 p.

Research output: Contribution to journalArticle

Trypanosoma
Trypanosoma evansi
Trypanosoma vivax
Trypanosomiasis
South America
2008

A systematic approach to understand the mechanism of action of the bisthiazolium compound T4 on the human malaria parasite, Plasmodium falciparum

Le Roch, K. G., Johnson, J. R., Ahiboh, H., Chung, D. W. D., Prudhomme, J., Plouffe, D., Henson, K., Zhou, Y., Witola, W., Yates, J. R., Mamoun, C. B., Winzeler, E. A. & Vial, H. Oct 30 2008 In : BMC Genomics. 9, 513

Research output: Contribution to journalArticle

Falciparum Malaria
Choline
Ethanolamine
Parasites
Plasmodium falciparum

Biochemical and genetic analysis of the phosphoethanolamine methyltransferase of the human malaria parasite Plasmodium falciparum

Reynolds, J. M., Takebe, S., Choi, J. Y., El Bissati, K., Witola, W. H., Bobenchik, A. M., Hoch, J. C., Voelker, D. R. & Mamoun, C. B. Mar 21 2008 In : Journal of Biological Chemistry. 283, 12, p. 7894-7900 7 p.

Research output: Contribution to journalArticle

Falciparum Malaria
Molecular Biology
Parasites
Methylation
Plasmodium falciparum

Detection of Trypanosoma brucei in field-captured tsetse flies and identification of host species fed on by the infected flies

Konnai, S., Mekata, H., Odbileg, R., Simuunza, M., Chembensof, M., Witola, W. H., Tembo, M. E., Chitambo, H., Inoue, N., Onuma, M. & Ohashi, K. Aug 1 2008 In : Vector-Borne and Zoonotic Diseases. 8, 4, p. 565-573 9 p.

Research output: Contribution to journalArticle

Tsetse Flies
Trypanosoma brucei brucei
Goats
Diptera
Trypanosomiasis
Carboxy-Lyases
Biosynthesis
Plasmodium falciparum
Phosphatidylcholines
Serine

Prevalence and source of trypanosome infections in field-captured vector flies (Glossina pallidipes) in southeastern Zambia

Mekata, H., Konnai, S., Simuunza, M., Chembensofu, M., Kano, R., Witola, W. H., Tembo, M. E., Chitambo, H., Inoue, N., Onuma, M. & Ohashi, K. Sep 2008 In : Journal of Veterinary Medical Science. 70, 9, p. 923-928 6 p.

Research output: Contribution to journalArticle

Glossina pallidipes
Tsetse Flies
Zambia
Trypanosomiasis
Trypanosoma congolense
2007

Choline induces transcriptional repression and proteasomal degradation of the malarial phosphoethanolamine methyltransferase

Witola, W. H. & Mamoun, C. B. Sep 2007 In : Eukaryotic Cell. 6, 9, p. 1618-1624 7 p.

Research output: Contribution to journalArticle

methyltransferases
choline
Choline
Serine
Carboxy-Lyases

Purification of components of the translation elongation factor complex of Plasmodium falciparum by tandem affinity purification

Takebe, S., Witola, W. H., Schimanski, B., Günzl, A. & Ben Mamoun, C. Apr 2007 In : Eukaryotic Cell. 6, 4, p. 584-591 8 p.

Research output: Contribution to journalArticle

Peptide Elongation Factors
Plasmodium falciparum
translation (genetics)
Proteins
proteins
2006

Acquisition and transmission of Theileria parva by vector tick, Rhipicephalus appendiculatus

Konnai, S., Imamura, S., Nakajima, C., Witola, W. H., Yamada, S., Simuunza, M., Nambota, A., Yasuda, J., Ohashi, K. & Onuma, M. Aug 1 2006 In : Acta Tropica. 99, 1, p. 34-41 8 p.

Research output: Contribution to journalArticle

Theileria parva
Rhipicephalus
Ticks
Theileriasis
Real-Time Polymerase Chain Reaction

Localization of the phosphoethanolamine methyltransferase of the human malaria parasite Plasmodium falciparum to the Golgi apparatus

Witola, W. H., Pessi, G., El Bissati, K., Reynolds, J. M. & Mamoun, C. B. Jul 28 2006 In : Journal of Biological Chemistry. 281, 30, p. 21305-21311 7 p.

Research output: Contribution to journalArticle

Falciparum Malaria
Golgi Apparatus
Carboxy-Lyases
Serine
Parasites

The effect of TAO expression on PCD-like phenomenon development and drug resistance in Trypanosoma brucei

Tsuda, A., Witola, W. H., Konnai, S., Ohashi, K. & Onuma, M. Jun 1 2006 In : Parasitology International. 55, 2, p. 135-142 8 p.

Research output: Contribution to journalArticle

Troleandomycin
Trypanosoma brucei brucei
Drug Resistance
Cell Death
Reactive Oxygen Species

The plasma membrane permese PfNT1 is essential for purine salvage in the human malaria parasite Plasmodium falciparum

El Bissati, K., Zufferey, R., Witola, W. H., Carter, N. S., Ullman, B. & Ben Mamoun, C. Jun 13 2006 In : Proceedings of the National Academy of Sciences of the United States of America. 103, 24, p. 9286-9291 6 p.

Research output: Contribution to journalArticle

Nucleoside Transport Proteins
Falciparum Malaria
Plasmodium falciparum
Parasites
Cell Membrane
2005

Acquired resistance to berenil in a cloned isolate of Trypanosoma evansi is associated with upregulation of a novel gene, TeDR40

Witola, W. H., Tsuda, A., Inoue, N., Ohashi, K. & Onuma, M. Nov 2005 In : Parasitology. 131, 5, p. 635-646 12 p.

Research output: Contribution to journalArticle

Trypanosoma
Trypanosoma evansi
Up-Regulation
drug resistance
Trypanosomiasis

Expression of a gene encoding Trypanosoma congolense putative Abc1 family protein is developmentally regulated

Baticados, W. N., Witola, W. H., Inoue, N., Kim, J. Y., Kuboki, N., Xuan, X., Yokoyama, N. & Sugimoto, C. Feb 2005 In : Journal of Veterinary Medical Science. 67, 2, p. 157-164 8 p.

Research output: Contribution to journalArticle

Trypanosoma congolense
bloodstream forms
Gene Expression
gene expression
Proteins

Expression of alternative oxidase inhibits programmed cell death-like phenomenon in bloodstream form of Trypanosoma brucei rhodesiense

Tsuda, A., Witola, W. H., Ohashi, K. & Onuma, M. Dec 1 2005 In : Parasitology International. 54, 4, p. 243-251 9 p.

Research output: Contribution to journalArticle

Trypanosoma brucei rhodesiense
Cell Death
Trypanosomiasis
Axenic Culture
African Trypanosomiasis

Genetic variability in ESAG6 genes among Trypanosoma evansi isolates and in comparison to other Trypanozoon members

Witola, W. H., Sarataphan, N., Inoue, N., Ohashi, K. & Onuma, M. Jan 1 2005 In : Acta Tropica. 93, 1, p. 63-73 11 p.

Research output: Contribution to journalArticle

Trypanosoma
Trypanosoma brucei brucei
Transferrin Receptors
Genes
Transferrins
2004

RNA-interference silencing of the adenosine transporter-1 gene in Trypanosoma evansi confers resistance to diminazene aceturate

Witola, W. H., Inoue, N., Ohashi, K. & Onuma, M. May 1 2004 In : Experimental Parasitology. 107, 1-2, p. 47-57 11 p.

Research output: Contribution to journalArticle

Trypanosoma
RNA Interference
Pentamidine
Genes
Untranslated Regions
2001

Demonstration of erythrophagocytosis in Trypanosoma congolense-infected goats

Witola, W. H. & Lovelace, C. E. A. Mar 20 2001 In : Veterinary parasitology. 96, 2, p. 115-126 12 p.

Research output: Contribution to journalArticle

Trypanosoma congolense
Goats
goats
anemia
Anemia
1997

Serum proteins changes in indigenous zambian goats with trypanosomosis

Witola, W. H. & Lovelace, C. E. A. 1997 In : FASEB Journal. 11, 9

Research output: Contribution to journalArticle

trypanosomiasis
Goats
Liver
blood proteins
Blood Proteins