Research Output 1997 2017

2017

Inherent oxidative stress in the Lewis rat is associated with resistance to toxoplasmosis

Witola, W. H., Kim, C. Y. & Zhang, X. 2017 In : Infection and Immunity. 85, 10, e00289-17

Research output: Research - peer-reviewArticle

Toxoplasmosis
Oxidative Stress
Toxoplasma
Reactive Oxygen Species
Glutathione Transferase

In vitro antagonistic and indifferent activity of combination of 3-deoxyanthocyanidins against Toxoplasma gondii

Abugri, D. A., Witola, W. H. & Jaynes, J. M. Dec 1 2017 In : Parasitology Research. 116, 12, p. 3387-3400 14 p.

Research output: Research - peer-reviewArticle

Toxoplasma
In Vitro Techniques
Toxoplasma gondii
Parasites
parasites

Targeted gene knockdown validates the essential role of lactate dehydrogenase in Cryptosporidium parvum

Witola, W. H., Zhang, X. & Kim, C. Y. Nov 1 2017 In : International Journal for Parasitology. 47, 13, p. 867-874 8 p.

Research output: Research - peer-reviewArticle

Gene Knockdown Techniques
Cryptosporidium parvum
L-Lactate Dehydrogenase
Morpholinos
Genes
2016

In vitro activity of Sorghum bicolor extracts, 3-deoxyanthocyanidins, against Toxoplasma gondii

Abugri, D. A., Witola, W. H., Jaynes, J. M. & Toufic, N. May 1 2016 In : Experimental Parasitology. 164, p. 12-19 8 p.

Research output: Research - peer-reviewArticle

Sorghum
Toxoplasma
In Vitro Techniques
ethyl acetate
Antiparasitic Agents
Haemonchus
Anthelmintics
In Vitro Techniques
phosphoethanolamine methyltransferase
Larva

Knockdown of phosphoethanolamine transmethylation enzymes decreases viability of Haemonchus contortus

Witola, W. H., Cooks-Fagbodun, S., Ordonez, A. R., Matthews, K., Abugri, D. A. & McHugh, M. Jun 15 2016 In : Veterinary Parasitology. 223, p. 1-6 6 p.

Research output: Research - peer-reviewArticle

Haemonchus
Enzymes
phosphoethanolamine methyltransferase
phosphorylethanolamine
Haemonchus contortus
2014

ALOX12 in human toxoplasmosis

Witola, W. H., Liu, S. R., Montpetit, A., Welti, R., Hypolite, M., Roth, M., Zhou, Y., Mui, E., Cesbron-Delauw, M. F., Fournie, G. J., Cavailles, P., Bisanz, C., Boyer, K., Withers, S., Gwendolyn Noble, A., Swisher, C. N., Heydemann, P. T., Rabiah, P., Muench, S. P. & McLeod, R. 2014 In : Infection and Immunity. 82, 7, p. 2670-2679 10 p.

Research output: Research - peer-reviewArticle

Toxoplasmosis
Genes
Hydroxyeicosatetraenoic Acids
Arachidonic Acid
Parasites

Silencing of GRA10 protein expression inhibits Toxoplasma gondii intracellular growth and development

Witola, W. H., Bauman, B., McHugh, M. & Matthews, K. 2014 In : Parasitology International. 63, 5, p. 651-658 8 p.

Research output: Research - peer-reviewArticle

Toxoplasma
Growth and Development
Proteins
Vacuoles
Parasites
2013

Isolation, cloning, and pathologic analysis of Trypanosoma evansi field isolates

Mekata, H., Konnai, S., Mingala, C. N., Abes, N. S., Gutierrez, C. A., Dargantes, A. P., Witola, W. H., Inoue, N., Onuma, M., Murata, S. & Ohashi, K. Apr 2013 In : Parasitology Research. 112, 4, p. 1513-1521 9 p.

Research output: Research - peer-reviewArticle

Trypanosoma
Virulence
Organism Cloning
Trypanosoma evansi
cattle

Plasmodium falciparum phosphoethanolamine methyltransferase is essential for malaria transmission

Bobenchik, A. M., Witola, W. H., Augagneur, Y., Lochlainn, L. N., Garg, A., Pachikara, N., Choi, J. Y., Zhao, Y. O., Usmani-Brown, S., Lee, A., Adjalley, S. H., Samanta, S., Fidock, D. A., Voelker, D. R., Fikrig, E. & Mamoun, C. B. Nov 5 2013 In : Proceedings of the National Academy of Sciences of the United States of America. 110, 45, p. 18262-18267 6 p.

Research output: Research - peer-reviewArticle

Plasmodium falciparum
Malaria
Parasites
phosphoethanolamine methyltransferase
Culicidae
2012

Kinetics of regulatory dendritic cells in inflammatory responses during Trypanosoma evansi infection

Mekata, H., Konnai, S., Mingala, C. N., Abes, N. S., Gutierrez, C. A., Dargantes, A. P., Witola, W. H., Inoue, N., Onuma, M., Murata, S. & Ohashi, K. Jun 2012 In : Parasite Immunology. 34, 6, p. 318-329 12 p.

Research output: Research - peer-reviewArticle

Trypanosoma
Dendritic Cells
Infection
Wasting Syndrome
Chemokines

Molecular target validation, antimicrobial delivery, and potential treatment of Toxoplasma gondii infections

Lai, B. S., Witola, W. H., El Bissati, K., Zhou, Y., Mui, E., Fomovska, A. & McLeod, R. Aug 28 2012 In : Proceedings of the National Academy of Sciences of the United States of America. 109, 35, p. 14182-14187 6 p.

Research output: Research - peer-reviewArticle

Toxoplasmosis
Peptides
Toxoplasma
Tetrahydrofolate Dehydrogenase
Oxidoreductases

Novel N-benzoyl-2-hydroxybenzamide disrupts unique parasite secretory pathway

Fomovska, A. , Huang, Q. , El Bissati, K. , Mui, E. J. , Witola, W. H. , Cheng, G. , Zhou, Y. , Sommerville, C. , Roberts, C. W. , Bettis, S. , Prigge, S. T. , Afanador, G. A. , Hickman, M. R. , Lee, P. J. , Leed, S. E. , Auschwitz, J. M. , Pieroni, M. , Stec, J. , Muench, S. P. , Rice, D. W. & 2 others Kozikowski, A. P. & McLeod, R. May 2012 In : Antimicrobial Agents and Chemotherapy. 56, 5, p. 2666-2682 17 p.

Research output: Research - peer-reviewArticle

Secretory Pathway
Toxoplasma
Parasites
2-hydroxybenzamide
Proteins

Synthesis, biological evaluation, and structure-activity relationships of N -benzoyl-2-hydroxybenzamides as agents active against P. falciparum (K1 strain), trypanosomes, and leishmania

Stec, J., Huang, Q., Pieroni, M., Kaiser, M., Fomovska, A., Mui, E., Witola, W. H., Bettis, S., McLeod, R., Brun, R. & Kozikowski, A. P. Apr 12 2012 In : Journal of Medicinal Chemistry. 55, 7, p. 3088-3100 13 p.

Research output: Research - peer-reviewArticle

Trypanosomiasis
Leishmania
Toxoplasma
Structure-Activity Relationship
2-hydroxybenzamide

Toxoplasma gondii HLA-B*0702-restricted GRA7 20-28 peptide with adjuvants and a universal helper T cell epitope elicits CD8 + T cells producing interferon-γ and reduces parasite burden in HLA-B*0702 mice

Cong, H., Mui, E. J., Witola, W. H., Sidney, J., Alexander, J., Sette, A., Maewal, A., El Bissati, K., Zhou, Y., Suzuki, Y., Lee, D., Woods, S., Sommerville, C., Henriquez, F. L., Roberts, C. W. & McLeod, R. Jan 1 2012 In : Human Immunology. 73, 1, p. 1-10 10 p.

Research output: Research - peer-reviewArticle

T-Lymphocyte Epitopes
Toxoplasma
Helper-Inducer T-Lymphocytes
Interferon-gamma
Parasites
2011

NALP1 influences susceptibility to human congenital toxoplasmosis, proinflammatory cytokine response, and fate of Toxoplasma gondii-infected monocytic cells

Witola, W. H., Mui, E., Hargrave, A., Liu, S., Hypolite, M., Montpetit, A., Cavailles, P., Bisanz, C., Cesbron-Delauw, M. F., Fournié, G. J. & McLeod, R. Feb 2011 In : Infection and Immunity. 79, 2, p. 756-766 11 p.

Research output: Research - peer-reviewArticle

Congenital Toxoplasmosis
Inflammasomes
Toxoplasma
Cytokines
Toxoplasmosis

Towards an immunosense vaccine to prevent toxoplasmosis: Protective Toxoplasma gondii epitopes restricted by HLA-A*0201

Cong, H., Mui, E. J., Witola, W. H., Sidney, J., Alexander, J., Sette, A., Maewal, A. & McLeod, R. Jan 17 2011 In : Vaccine. 29, 4, p. 754-762 9 p.

Research output: Research - peer-reviewArticle

Toxoplasmosis
Toxoplasma
Epitopes
Vaccines
Peptides
2010
T-Lymphocyte Epitopes
HLA-A Antigens
Toxoplasma
Computational Biology
Transgenic Mice

Identification of T. gondii epitopes, adjuvants, and host genetic factors that influence protection of mice and humans

Tan, T. G., Mui, E., Cong, H., Witola, W. H., Montpetit, A., Muench, S. P., Sidney, J., Alexander, J., Sette, A., Grigg, M. E., Maewal, A. & McLeod, R. May 21 2010 In : Vaccine. 28, 23, p. 3977-3989 13 p.

Research output: Research - peer-reviewArticle

epitopes
adjuvants
mice
Epitopes
T-lymphocytes

P2X7 receptor-mediated killing of an intracellular parasite, Toxoplasma gondii, by human and murine macrophages

Lees, M. P., Fuller, S. J., McLeod, R., Boulter, N. R., Miller, C. M., Zakrzewski, A. M., Mui, E. J., Witola, W. H., Coyne, J. J., Hargrave, A. C., Jamieson, S. E., Blackwell, J. M., Wiley, J. S. & Smith, N. C. Jun 15 2010 In : Journal of Immunology. 184, 12, p. 7040-7046 7 p.

Research output: Research - peer-reviewArticle

Toxoplasma
Parasites
Macrophages
Single Nucleotide Polymorphism
Adenosine Triphosphate

T. gondii RP promoters & knockdown reveal molecular pathways associated with proliferation and cell-cycle arrest

Hutson, S. L., Mui, E., Kinsley, K., Witola, W. H., Behnke, M. S., El Bissati, K., Muench, S. P., Rohrman, B., Liu, S. R., Wollmann, R., Ogata, Y., Sarkeshik, A., Yates, J. R. & McLeod, R. 2010 In : PLoS ONE. 5, 11, e14057

Research output: Research - peer-reviewArticle

ribosomal proteins
promoter regions
parasites
cell cycle checkpoints
Ribosomal Proteins

The malarial CDK Pfmrk and its effector PfMAT1 phosphorylate DNA replication proteins and co-localize in the nucleus

Jirage, D., Chen, Y., Caridha, D., O'Neil, M. T., Eyase, F., Witola, W. H., Mamoun, C. B. & Waters, N. C. Jul 2010 In : Molecular and Biochemical Parasitology. 172, 1, p. 9-18 10 p.

Research output: Research - peer-reviewArticle

Cyclin-Dependent Kinases
DNA Replication
Parasites
Proteins
Falciparum Malaria
2009

Development and application of a quantitative real-time PCR for the diagnosis of Surra in water buffaloes

Konnai, S., Mekata, H., Mingala, C. N., Abes, N. S., Gutierrez, C. A., Herrera, J. R. V., Dargantes, A. P., Witola, W. H., Cruz, L. C., Inoue, N., Onuma, M. & Ohashi, K. Jul 2009 In : Infection, Genetics and Evolution. 9, 4, p. 449-452 4 p.

Research output: Research - peer-reviewArticle

surra
Trypanosoma evansi
buffaloes
quantitative polymerase chain reaction
blood

Expression and activity of glycogen synthase kinase during vitellogenesis and embryogenesis of Rhipicephalus (Boophilus) microplus

Logullo, C., Witola, W. H., Andrade, C., Abreu, L., Gomes, J., da Silva Vaz, I., Imamura, S., Konnai, S., Ohashi, K. & Onuma, M. May 12 2009 In : Veterinary Parasitology. 161, 3-4, p. 261-269 9 p.

Research output: Research - peer-reviewArticle

Rhipicephalus microplus
vitellogenesis
glycogen
embryogenesis
glycogen synthase kinases

Molecular detection of trypanosomes in cattle in South America and genetic diversity of Trypanosoma evansi based on expression-site-associated gene 6

Mekata, H., Konnai, S., Witola, W. H., Inoue, N., Onuma, M. & Ohashi, K. Dec 2009 In : Infection, Genetics and Evolution. 9, 6, p. 1301-1305 5 p.

Research output: Research - peer-reviewArticle

cattle
gene
infection
South America
detection
2008

A systematic approach to understand the mechanism of action of the bisthiazolium compound T4 on the human malaria parasite, Plasmodium falciparum

Le Roch, K. G., Johnson, J. R., Ahiboh, H., Chung, D. W. D., Prudhomme, J., Plouffe, D., Henson, K., Zhou, Y., Witola, W., Yates, J. R., Mamoun, C. B., Winzeler, E. A. & Vial, H. Oct 30 2008 In : BMC Genomics. 9, 513

Research output: Research - peer-reviewArticle

Falciparum Malaria
Choline
Parasites
1,12-bis(4-methyl-5-(2-methoxyethyl)thiazol-3-ium-3-yl)dodecane
Ethanolamine

Biochemical and genetic analysis of the phosphoethanolamine methyltransferase of the human malaria parasite Plasmodium falciparum

Reynolds, J. M., Takebe, S., Choi, J. Y., El Bissati, K., Witola, W. H., Bobenchik, A. M., Hoch, J. C., Voelker, D. R. & Mamoun, C. B. Mar 21 2008 In : Journal of Biological Chemistry. 283, 12, p. 7894-7900 7 p.

Research output: Research - peer-reviewArticle

Falciparum Malaria
Molecular Biology
Parasites
Enzymes
phosphoethanolamine methyltransferase

Detection of Trypanosoma brucei in field-captured tsetse flies and identification of host species fed on by the infected flies

Konnai, S., Mekata, H., Odbileg, R., Simuunza, M., Chembensof, M., Witola, W. H., Tembo, M. E., Chitambo, H., Inoue, N., Onuma, M. & Ohashi, K. Aug 1 2008 In : Vector-Borne and Zoonotic Diseases. 8, 4, p. 565-573 9 p.

Research output: Research - peer-reviewArticle

Tsetse Flies
Trypanosoma brucei brucei
Goats
Diptera
Trypanosomiasis
Carboxy-Lyases
Plasmodium falciparum
Phosphatidylcholines
Serine
Parasites

Prevalence and source of trypanosome infections in field-captured vector flies (Glossina pallidipes) in southeastern Zambia

Mekata, H., Konnai, S., Simuunza, M., Chembensofu, M., Kano, R., Witola, W. H., Tembo, M. E., Chitambo, H., Inoue, N., Onuma, M. & Ohashi, K. Sep 2008 In : Journal of Veterinary Medical Science. 70, 9, p. 923-928 6 p.

Research output: Research - peer-reviewArticle

Tsetse Flies
Zambia
Trypanosomiasis
Diptera
Infection
2007

Choline induces transcriptional repression and proteasomal degradation of the malarial phosphoethanolamine methyltransferase

Witola, W. H. & Mamoun, C. B. Sep 2007 In : Eukaryotic Cell. 6, 9, p. 1618-1624 7 p.

Research output: Research - peer-reviewArticle

Choline
phosphoethanolamine methyltransferase
methyltransferases
choline
degradation

Purification of components of the translation elongation factor complex of Plasmodium falciparum by tandem affinity purification

Takebe, S., Witola, W. H., Schimanski, B., Günzl, A. & Ben Mamoun, C. Apr 2007 In : Eukaryotic Cell. 6, 4, p. 584-591 8 p.

Research output: Research - peer-reviewArticle

Peptide Elongation Factors
Plasmodium falciparum
Proteins
translation (genetics)
proteins
2006

Acquisition and transmission of Theileria parva by vector tick, Rhipicephalus appendiculatus

Konnai, S., Imamura, S., Nakajima, C., Witola, W. H., Yamada, S., Simuunza, M., Nambota, A., Yasuda, J., Ohashi, K. & Onuma, M. Aug 2006 In : Acta Tropica. 99, 1, p. 34-41 8 p.

Research output: Research - peer-reviewArticle

Theileria parva
Rhipicephalus
Ticks
Real-Time Polymerase Chain Reaction
Infection

Localization of the phosphoethanolamine methyltransferase of the human malaria parasite Plasmodium falciparum to the Golgi apparatus

Witola, W. H., Pessi, G., El Bissati, K., Reynolds, J. M. & Mamoun, C. B. Jul 28 2006 In : Journal of Biological Chemistry. 281, 30, p. 21305-21311 7 p.

Research output: Research - peer-reviewArticle

Falciparum Malaria
Golgi Apparatus
Serine
Parasites
phosphoethanolamine methyltransferase

The effect of TAO expression on PCD-like phenomenon development and drug resistance in Trypanosoma brucei

Tsuda, A., Witola, W. H., Konnai, S., Ohashi, K. & Onuma, M. Jun 2006 In : Parasitology International. 55, 2, p. 135-142 8 p.

Research output: Research - peer-reviewArticle

Troleandomycin
Trypanosoma brucei brucei
Drug Resistance
Cell Death
quinapyramine

The plasma membrane permese PfNT1 is essential for purine salvage in the human malaria parasite Plasmodium falciparum

El Bissati, K., Zufferey, R., Witola, W. H., Carter, N. S., Ullman, B. & Ben Mamoun, C. Jun 13 2006 In : Proceedings of the National Academy of Sciences of the United States of America. 103, 24, p. 9286-9291 6 p.

Research output: Research - peer-reviewArticle

Nucleoside Transport Proteins
Falciparum Malaria
Plasmodium falciparum
Parasites
Cell Membrane
2005

Acquired resistance to berenil in a cloned isolate of Trypanosoma evansi is associated with upregulation of a novel gene, TeDR40

Witola, W. H., Tsuda, A., Inoue, N., Ohashi, K. & Onuma, M. Nov 2005 In : Parasitology. 131, 5, p. 635-646 12 p.

Research output: Research - peer-reviewArticle

Trypanosoma
Up-Regulation
Genes
diminazene aceturate
Trypanosoma evansi

Expression of a gene encoding Trypanosoma congolense putative Abc1 family protein is developmentally regulated

Baticados, W. N., Witola, W. H., Inoue, N., Kim, J. Y., Kuboki, N., Xuan, X., Yokoyama, N. & Sugimoto, C. Feb 2005 In : Journal of Veterinary Medical Science. 67, 2, p. 157-164 8 p.

Research output: Research - peer-reviewArticle

Trypanosoma congolense
gene expression
proteins
Gene Expression
Proteins

Expression of alternative oxidase inhibits programmed cell death-like phenomenon in bloodstream form of Trypanosoma brucei rhodesiense

Tsuda, A., Witola, W. H., Ohashi, K. & Onuma, M. Dec 2005 In : Parasitology International. 54, 4, p. 243-251 9 p.

Research output: Research - peer-reviewArticle

Trypanosoma brucei rhodesiense
Cell Death
alternative oxidase
Trypanosomiasis
Temperature

Genetic variability in ESAG6 genes among Trypanosoma evansi isolates and in comparison to other Trypanozoon members

Witola, W. H., Sarataphan, N., Inoue, N., Ohashi, K. & Onuma, M. Jan 2005 In : Acta Tropica. 93, 1, p. 63-73 11 p.

Research output: Research - peer-reviewArticle

Trypanosoma
Genes
Trypanosoma brucei brucei
Transferrin Receptors
Transferrins
2004

RNA-interference silencing of the adenosine transporter-1 gene in Trypanosoma evansi confers resistance to diminazene aceturate

Witola, W. H., Inoue, N., Ohashi, K. & Onuma, M. May 2004 In : Experimental Parasitology. 107, 1-2, p. 47-57 11 p.

Research output: Research - peer-reviewArticle

Trypanosoma
RNA Interference
Genes
diminazene aceturate
adenosine transporter
2001

Demonstration of erythrophagocytosis in Trypanosoma congolense-infected goats

Witola, W. H. & Lovelace, C. E. A. Mar 20 2001 In : Veterinary Parasitology. 96, 2, p. 115-126 12 p.

Research output: Research - peer-reviewArticle

Trypanosoma congolense
goats
Goats
infection
Infection
1997

Serum proteins changes in indigenous zambian goats with trypanosomosis

Witola, W. H. & Lovelace, C. E. A. 1997 In : FASEB Journal. 11, 9

Research output: Research - peer-reviewArticle

trypanosomiasis
blood proteins
goats
Liver
Blood Proteins