Wilfred Adrianus van der Donk

1990 …2019
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Research Output 1990 2019

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2019

Assessing the Flexibility of the Prochlorosin 2.8 Scaffold for Bioengineering Applications

Hegemann, J. D., Bobeica, S. C., Walker, M. C., Bothwell, I. R. & van der Donk, W. A., May 17 2019, In : ACS synthetic biology. 8, 5, p. 1204-1214 11 p.

Research output: Contribution to journalArticle

Bioengineering
Scaffolds
Peptides
Epitopes
Cyclization

CylA is a sequence-specific protease involved in toxin biosynthesis

Tang, W., Bobeica, S. C., Wang, L. & van der Donk, W. A., Mar 29 2019, In : Journal of Industrial Microbiology and Biotechnology. 46, 3-4, p. 537-549 13 p.

Research output: Contribution to journalArticle

Perforin
Biosynthesis
Peptide Hydrolases
Peptides
Subtilisin

Insights into AMS/PCAT transporters from biochemical and structural characterization of a double Glycine motif protease

Bobeica, S. C., Dong, S. H., Huo, L., Mazo, N., McLaughlin, M. I., Jiménez-Osés, G., Nair, S. K. & van der Donk, W. A., Jan 14 2019, In : eLife. 8

Research output: Contribution to journalArticle

Protein Sorting Signals
Glycine
Peptide Hydrolases
Bacteria
Peptides

Investigations into the Mechanism of Action of Sublancin

Wu, C., Biswas, S., Garcia De Gonzalo, C. V. & van der Donk, W. A., Mar 8 2019, In : ACS Infectious Diseases. 5, 3, p. 454-459 6 p.

Research output: Contribution to journalArticle

Glucose
Biological Products
DNA Replication
Cell Wall
Carbon

Use of the dehydrophos biosynthetic enzymes to prepare antimicrobial analogs of alaphosphin

Bougioukou, D. J., Ting, C. P., Peck, S. C., Mukherjee, S. & van der Donk, W. A., Jan 1 2019, In : Organic and Biomolecular Chemistry. 17, 4, p. 822-829 8 p.

Research output: Contribution to journalArticle

enzymes
analogs
Enzymes
RNA, Transfer, Leu
peptides
2018
Isotopes
isotope
kinetics
Kinetics
Hydrides

A lanthipeptide library used to identify a protein-protein interaction inhibitor article

Yang, X., Lennard, K. R., He, C., Walker, M. C., Ball, A. T., Doigneaux, C., Tavassoli, A. & van der Donk, W. A., Apr 1 2018, In : Nature chemical biology. 14, 4, p. 375-380 6 p.

Research output: Contribution to journalArticle

Libraries
Proteins
Human Immunodeficiency Virus Proteins
Ligases
Biological Products

Characterization of Leader Peptide Binding during Catalysis by the Nisin Dehydratase NisB

Repka, L. M., Hetrick, K. J., Chee, S. H. & van der Donk, W. A., Mar 28 2018, In : Journal of the American Chemical Society. 140, 12, p. 4200-4203 4 p.

Research output: Contribution to journalArticle

Hydro-Lyases
Nisin
catalysis
Protein Sorting Signals
Catalysis

Development and Application of Yeast and Phage Display of Diverse Lanthipeptides

Hetrick, K. J., Walker, M. C. & van der Donk, W. A., Apr 25 2018, In : ACS Central Science. 4, 4, p. 458-467 10 p.

Research output: Contribution to journalArticle

Bacteriophages
Yeast
Display devices
Peptides
Bioactivity

Elucidation of the roles of conserved residues in the biosynthesis of the lasso peptide paeninodin

Hegemann, J. D., Schwalen, C. J., Mitchell, D. A. & van der Donk, W. A., Jan 1 2018, In : Chemical Communications. 54, 65, p. 9007-9010 4 p.

Research output: Contribution to journalArticle

Biosynthesis
Peptides
Assays
Proteolysis
Substrates

Glutamic acid is a carrier for hydrazine during the biosyntheses of fosfazinomycin and kinamycin

Wang, K. K. A., Ng, T. L., Wang, P., Huang, Z., Balskus, E. P. & van der Donk, W. A., Dec 1 2018, In : Nature communications. 9, 1, 3687.

Research output: Contribution to journalArticle

hydrazine
glutamic acid
biosynthesis
Biosynthesis
hydrazines

Incorporation of Nonproteinogenic Amino Acids in Class i and II Lantibiotics

Kakkar, N., Perez, J. G., Liu, W. R., Jewett, M. C. & van der Donk, W. A., Apr 20 2018, In : ACS chemical biology. 13, 4, p. 951-957 7 p.

Research output: Contribution to journalArticle

Bacteriocins
Amino Acids
Nisin
Amber
Terminator Codon

Investigation of Amide Bond Formation during Dehydrophos Biosynthesis

Ulrich, E. C., Bougioukou, D. J. & van der Donk, W. A., Mar 16 2018, In : ACS chemical biology. 13, 3, p. 537-541 5 p.

Research output: Contribution to journalArticle

Biosynthesis
Transfer RNA
Amides
RNA, Transfer, Leu
Enzymes

Investigation of Substrate Recognition and Biosynthesis in Class IV Lanthipeptide Systems

Hegemann, J. D. & van der Donk, W. A., May 2 2018, In : Journal of the American Chemical Society. 140, 17, p. 5743-5754 12 p.

Research output: Contribution to journalArticle

Biosynthesis
peptide
Peptides
Enzymes
substrate

Lanthionine synthetase C-like protein 2 (LanCL2) is important for adipogenic differentiation

Dutta, D., Lai, K. Y., Reyes-Ordoñez, A., Chen, J. & van der Donk, W. A., Jan 1 2018, In : Journal of Lipid Research. 59, 8, p. 1433-1445 13 p.

Research output: Contribution to journalArticle

Peroxisome Proliferator-Activated Receptors
Ligases
Protein C
CCAAT-Enhancer-Binding Proteins
Proteins

Rapid Discovery of Glycocins through Pathway Refactoring in Escherichia coli

Ren, H., Biswas, S., Ho, S., van der Donk, W. A. & Zhao, H., Jan 1 2018, (Accepted/In press) In : ACS Chemical Biology.

Research output: Contribution to journalArticle

Bacteriocins
Escherichia coli
Genes
Anti-Bacterial Agents
Glycosylation

Rapid Screening of Lanthipeptide Analogs via In-Colony Removal of Leader Peptides in Escherichia coli

Si, T., Tian, Q., Min, Y., Zhang, L., Sweedler, J. V., van der Donk, W. A. & Zhao, H., Sep 26 2018, In : Journal of the American Chemical Society. 140, 38, p. 11884-11888 5 p.

Research output: Contribution to journalArticle

Protein Sorting Signals
peptide
Escherichia coli
Screening
Synthetic Biology

Stereospecific Radical-Mediated B12-Dependent Methyl Transfer by the Fosfomycin Biosynthesis Enzyme Fom3

McLaughlin, M. I. & van der Donk, W. A., Aug 21 2018, In : Biochemistry. 57, 33, p. 4967-4971 5 p.

Research output: Contribution to journalArticle

Fosfomycin
Cytidine Monophosphate
Biosynthesis
Vitamin B 12
Enzymes

Substrate-assisted enzymatic formation of lysinoalanine in duramycin

An, L., Cogan, D. P., Navo, C. D., Jiménez-Osés, G., Nair, S. K. & van der Donk, W. A., Oct 1 2018, In : Nature chemical biology. 14, 10, p. 928-933 6 p.

Research output: Contribution to journalArticle

Lysinoalanine
Viperidae
Catalysis
Cystic Fibrosis
duramycin
2017

Characterization of Two Late-Stage Enzymes Involved in Fosfomycin Biosynthesis in Pseudomonads

Olivares, P., Ulrich, E. C., Chekan, J. R., van der Donk, W. A. & Nair, S. K., Feb 17 2017, In : ACS chemical biology. 12, 2, p. 456-463 8 p.

Research output: Contribution to journalArticle

Fosfomycin
Biosynthesis
Enzymes
Anti-Bacterial Agents
Pseudomonas syringae

Chimeric Leader Peptides for the Generation of Non-Natural Hybrid RiPP Products

Burkhart, B. J., Kakkar, N., Hudson, G. A., van der Donk, W. A. & Mitchell, D. A., Jun 28 2017, In : ACS Central Science. 3, 6, p. 629-638 10 p.

Research output: Contribution to journalArticle

Protein Sorting Signals
Enzymes
Peptides
Biosynthesis
Substrates

Evolutionary radiation of lanthipeptides in marine cyanobacteria

Cubillos-Ruiz, A., Berta-Thompson, J. W., Becker, J. W., van der Donk, W. A. & Chisholm, S. W., Jul 3 2017, In : Proceedings of the National Academy of Sciences of the United States of America. 114, 27, p. E5424-E5433

Research output: Contribution to journalArticle

Cyanobacteria
Ligases
Radiation
Cyclic Peptides
Peptides

Insights into the biosynthesis of duramycin

Huo, L., Ökesli, A., Zhao, M. & van der Donk, W. A., Jan 1 2017, In : Applied and environmental microbiology. 83, 3, e02698-16.

Research output: Contribution to journalArticle

phosphatidylethanolamines
peptide
biosynthesis
aspartic acid
Streptomyces cinnamoneus

LanCL proteins are not Involved in Lanthionine Synthesis in Mammals

He, C., Zeng, M., Dutta, D., Koh, T. H., Chen, J. & Van Der Donk, W. A., Jan 20 2017, In : Scientific Reports. 7, 40980.

Research output: Contribution to journalArticle

mammals
synthesis
biosynthesis
brain
proteins

Mechanism of a Class C Radical S-Adenosyl- l -methionine Thiazole Methyl Transferase

Zhang, Z., Mahanta, N., Hudson, G. A., Mitchell, D. A. & van der Donk, W. A., Dec 27 2017, In : Journal of the American Chemical Society. 139, 51, p. 18623-18631 9 p.

Research output: Contribution to journalArticle

Thiazoles
Methyltransferases
Transferases
Methionine
Hydrogen

Mechanistic Understanding of Lanthipeptide Biosynthetic Enzymes

Repka, L. M., Chekan, J. R., Nair, S. K. & van der Donk, W. A., Apr 26 2017, In : Chemical reviews. 117, 8, p. 5457-5520 64 p.

Research output: Contribution to journalArticle

Sulfides
Bioactivity
Bacteriocins
Peptides
Cyclization

O-H activation by an unexpected ferryl intermediate during catalysis by 2-hydroxyethylphosphonate dioxygenase

Peck, S. C., Wang, C., Dassama, L. M. K., Zhang, B., Guo, Y., Rajakovich, L. J., Martin Bollinger, J., Krebs, C. & van der Donk, W. A., Feb 8 2017, In : Journal of the American Chemical Society. 139, 5, p. 2045-2052 8 p.

Research output: Contribution to journalArticle

Dioxygenases
catalysis
Catalysis
glufosinate
Chemical activation
Azurin
Electron transitions
Amides
Peptides
Electronic structure

Reconstitution and Substrate Specificity of the Radical S-Adenosyl-methionine Thiazole C-Methyltransferase in Thiomuracin Biosynthesis

Mahanta, N., Zhang, Z., Hudson, G. A., van der Donk, W. A. & Mitchell, D. A., Mar 29 2017, In : Journal of the American Chemical Society. 139, 12, p. 4310-4313 4 p.

Research output: Contribution to journalArticle

Thiazoles
methionine S-methyltransferase
Biosynthesis
Methyltransferases
Substrate Specificity

Ribosomal Natural Products, Tailored to Fit

Funk, M. A. & van der Donk, W. A., Jul 18 2017, In : Accounts of chemical research. 50, 7, p. 1577-1586 10 p.

Research output: Contribution to journalArticle

Biological Products
Bioactivity
Amino Acids
Peptides
Enzymes

Structural basis for methylphosphonate biosynthesis

Born, D. A., Ulrich, E. C., Ju, K. S., Peck, S. C., van der Donk, W. A. & Drennan, C. L., Dec 8 2017, In : Science. 358, 6368, p. 1336-1339 4 p.

Research output: Contribution to journalArticle

Methane
Glutamine
Oceans and Seas
Phosphorus
methylphosphonic acid
Peptide Biosynthesis
Cycloaddition Reaction
Anti-Bacterial Agents
Catalytic Domain
Organic Chemistry

Structure-Activity Relationships of the S-Linked Glycocin Sublancin

Biswas, S., Garcia De Gonzalo, C. V., Repka, L. M. & van der Donk, W. A., Dec 15 2017, In : ACS chemical biology. 12, 12, p. 2965-2969 5 p.

Research output: Contribution to journalArticle

Structure-Activity Relationship
Bioactivity
Biological Products
Biosynthetic Pathways
Disulfides

Two Flavoenzymes Catalyze the Post-Translational Generation of 5-Chlorotryptophan and 2-Aminovinyl-Cysteine during NAI-107 Biosynthesis

Ortega, M. A., Cogan, D. P., Mukherjee, S., Garg, N., Li, B., Thibodeaux, G. N., Maffioli, S. I., Donadio, S., Sosio, M., Escano, J., Smith, L., Nair, S. K. & van der Donk, W. A., Feb 17 2017, In : ACS chemical biology. 12, 2, p. 548-557 10 p.

Research output: Contribution to journalArticle

Biosynthesis
Cysteine
Bacteriocins
Substrates
Bioinformatics
2016

Biosynthetic investigation of phomopsins reveals a widespread pathway for ribosomal natural products in Ascomycetes

Ding, W., Liu, W. Q., Jia, Y., Li, Y., van der Donk, W. A. & Zhang, Q., Mar 29 2016, In : Proceedings of the National Academy of Sciences of the United States of America. 113, 13, p. 3521-3526 6 p.

Research output: Contribution to journalArticle

Ascomycota
Biological Products
Methyltransferases
Fungi
Antimitotic Agents

Biosynthetic Timing and Substrate Specificity for the Thiopeptide Thiomuracin

Zhang, Z., Hudson, G. A., Mahanta, N., Tietz, J. I., van der Donk, W. A. & Mitchell, D. A., Dec 7 2016, In : Journal of the American Chemical Society. 138, 48, p. 15511-15514 4 p.

Research output: Contribution to journalArticle

Alkenes
Protein Sorting Signals
Substrate Specificity
peptide
Peptides

Characterization of the stereochemical configuration of lanthionines formed by the lanthipeptide synthetase GeoM

Garg, N., Goto, Y., Chen, T. & van der Donk, W. A., Nov 1 2016, In : Biopolymers. 106, 6, p. 834-842 9 p.

Research output: Contribution to journalArticle

Ligases
Peptides
Peptide Synthases
Perforin
Bacteriocins
Bacteriocins
peptide
Peptides
Amino acids
amino acid

Leader Peptide Establishes Dehydration Order, Promotes Efficiency, and Ensures Fidelity during Lacticin 481 Biosynthesis

Thibodeaux, C. J., Wagoner, J., Yu, Y. & van der Donk, W. A., May 25 2016, In : Journal of the American Chemical Society. 138, 20, p. 6436-6444 9 p.

Research output: Contribution to journalArticle

Chemical bonds
Biosynthesis
Ligases
Protein Sorting Signals
Dehydration

New insights into the biosynthesis of fosfazinomycin

Huang, Z., Wang, K. K. A. & van der Donk, W. A., Jan 1 2016, In : Chemical Science. 7, 8, p. 5219-5223 5 p.

Research output: Contribution to journalArticle

Biosynthesis
Adenylosuccinate Lyase
RNA, Transfer, Val
Nitrous Acid
Acetylation
Bacteriocins
Ruminants
Bioactivity
Bacteria
Peptides

Structure and tRNA Specificity of MibB, a Lantibiotic Dehydratase from Actinobacteria Involved in NAI-107 Biosynthesis

Ortega, M. A., Hao, Y., Walker, M. C., Donadio, S., Sosio, M., Nair, S. K. & van der Donk, W., Mar 17 2016, In : Cell chemical biology. 23, 3, p. 370-380 11 p.

Research output: Contribution to journalArticle

RNA, Transfer, Glu
Hydro-Lyases
Bacteriocins
Actinobacteria
Biosynthesis

Synthesis and Bioactivity of Diastereomers of the Virulence Lanthipeptide Cytolysin

Mukherjee, S., Huo, L., Thibodeaux, G. N. & van der Donk, W. A., Dec 2 2016, In : Organic Letters. 18, 23, p. 6188-6191 4 p.

Research output: Contribution to journalArticle

virulence
Perforin
Stereochemistry
Bioactivity
Virulence

The Enterococcal Cytolysin Synthetase Coevolves with Substrate for Stereoselective Lanthionine Synthesis

Tang, W., Thibodeaux, G. N. & van der Donk, W. A., Sep 16 2016, In : ACS chemical biology. 11, 9, p. 2438-2446 9 p.

Research output: Contribution to journalArticle

Perforin
Ligases
Stereoselectivity
Substrates
Peptides
2015

A common late-stage intermediate in catalysis by 2-hydroxyethyl-phosphonate dioxygenase and methylphosphonate synthase

Peck, S. C., Chekan, J. R., Ulrich, E. C., Nair, S. K. & van der Donk, W. A., Jan 1 2015, In : Journal of the American Chemical Society. 137, 9, p. 3217-3220 4 p.

Research output: Contribution to journalArticle

Dioxygenases
catalysis
Catalysis
Isotopes
Iron

An unexpected role for ergothioneine

Huang, Z. & van der Donk, W. A., Dec 1 2015, In : National Science Review. 2, 4, p. 382-383 2 p.

Research output: Contribution to journalArticle

Applications of the class II lanthipeptide protease LicP for sequence-specific, traceless peptide bond cleavage

Tang, W., Dong, S. H., Repka, L. M., He, C., Nair, S. K. & van der Donk, W. A., Sep 2 2015, In : Chemical Science. 6, 11, p. 6270-6279 10 p.

Research output: Contribution to journalArticle

Peptide Hydrolases
Peptides
Biosynthesis
Subtilisin
Bacteriocins

Biosynthesis of fosfazinomycin is a convergent process

Huang, Z., Wang, K. K. A., Lee, J. & van der Donk, W. A., Feb 1 2015, In : Chemical Science. 6, 2, p. 1282-1287 6 p.

Research output: Contribution to journalArticle

Biosynthesis
Phosphonoacetic Acid
Methyltransferases
Acetates
Dioxygenases

Discovery of phosphonic acid natural products by mining the genomes of 10,000 actinomycetes

Ju, K. S., Gao, J., Doroghazi, J. R., Wang, K. K. A., Thibodeaux, C. J., Li, S., Metzger, E., Fudala, J., Su, J., Zhang, J. K., Lee, J., Cioni, J. P., Evans, B. S., Hirota, R., Labeda, D. P., van der Donk, W. A. & Metcalf, W. W., Sep 29 2015, In : Proceedings of the National Academy of Sciences of the United States of America. 112, 39, p. 12175-12180 6 p.

Research output: Contribution to journalArticle

Actinobacteria
Organophosphonates
Biological Products
Genome
Phosphorous Acids

Facile Removal of Leader Peptides from Lanthipeptides by Incorporation of a Hydroxy Acid

Bindman, N. A., Bobeica, S. C., Liu, W. R. & van der Donk, W. A., Jun 10 2015, In : Journal of the American Chemical Society. 137, 22, p. 6975-6978 4 p.

Research output: Contribution to journalArticle

Hydroxy Acids
Protein Sorting Signals
peptide
Peptides
Acids