Stephen G Sligar

2024

An effective vaccine against influenza A virus based on the matrix protein 2 (M2)

Zuckermann, F. A., Grinkova, Y. V., Husmann, R. J., Pires-Alves, M., Storms, S., Chen, W. Y. & Sligar, S. G., Nov 2024, In: Veterinary Microbiology. 298, 110245.

Research output: Contribution to journal › Article › peer-review

Open Access

Cryoradiolysis of oxygenated cytochrome P450 17A1 with lyase substrates generates expected products

Usai, R., Denisov, I. G., Sligar, S. G. & Kincaid, J. R., Aug 2024, In: Journal of Inorganic Biochemistry. 257, 112582.

Research output: Contribution to journal › Article › peer-review

Optical annealing of peroxo-ferric intermediates in CYP17A1 and product formation.

Denisov, I. G., Grinkova, Y. V. & Sligar, S. G., Nov 2024, In: Journal of Inorganic Biochemistry. 260, 112701.

Research output: Contribution to journal › Article › peer-review

Selective α-Hydroxyketone Formation and Subsequent C-C Bond Cleavage by Cytochrome P450 Monooxygenase Enzymes

Lee, J. H. Z., Coleman, T., Mclean, M. A., Podgorski, M. N., Hayball, E. F., Stone, I. S. J., Bruning, J. B., Whelan, F., De Voss, J. J., Sligar, S. G. & Bell, S. G., Jun 7 2024, In: ACS Catalysis. 14, 11, p. 8958-8971 14 p.

Research output: Contribution to journal › Article › peer-review

2023

Engineering C-C Bond Cleavage Activity into a P450 Monooxygenase Enzyme

Miller, J. C., Lee, J. H. Z., Mclean, M. A., Chao, R. R., Stone, I. S. J., Pukala, T. L., Bruning, J. B., De Voss, J. J., Schuler, M. A., Sligar, S. G. & Bell, S. G., Apr 26 2023, In: Journal of the American Chemical Society. 145, 16, p. 9207-9222 16 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Revealing KRas4b topology on the membrane surface

Shree, S., McLean, M. A., Stephen, A. G. & Sligar, S. G., Oct 20 2023, In: Biochemical and Biophysical Research Communications. 678, p. 122-127 6 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Solvent isotope effects in the catalytic cycle of P450 CYP17A1: Computational modeling of the hydroxylation and lyase reactions

Denisov, I. G. & Sligar, S. G., Jun 2023, In: Journal of Inorganic Biochemistry. 243, 112202.

Research output: Contribution to journal › Article › peer-review

Open Access

2022

Importance of Asparagine 202 in Manipulating Active Site Structure and Substrate Preference for Human CYP17A1

Liu, Y., Denisov, I., Gregory, M., Sligar, S. G. & Kincaid, J. R., Apr 5 2022, In: Biochemistry. 61, 7, p. 583-594 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Midazolam as a Probe for Heterotropic Drug-Drug Interactions Mediated by CYP3A4

Denisov, I. G., Grinkova, Y. V., McLean, M. A., Camp, T. & Sligar, S. G., Jun 2022, In: Biomolecules. 12, 6, 853.

Research output: Contribution to journal › Article › peer-review

Open Access

2021

Mechanism of the Clinically Relevant E305G Mutation in Human P450 CYP17A1

Liu, Y., Grinkova, Y., Gregory, M. C., Denisov, I. G., Kincaid, J. R. & Sligar, S. G., Nov 2 2021, In: Biochemistry. 60, 43, p. 3262-3271 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Midazolam as a Probe for Drug-Drug Interactions Mediated by CYP3A4: Homotropic Allosteric Mechanism of Site-Specific Hydroxylation

Denisov, I. G., Grinkova, Y. V., Camp, T., McLean, M. A. & Sligar, S. G., Jun 1 2021, In: Biochemistry. 60, 21, p. 1670-1681 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Substrate-Specific Allosteric Effects on the Enhancement of CYP17A1 Lyase Efficiency by Cytochrome b₅

Liu, Y., Denisov, I. G., Sligar, S. G. & Kincaid, J. R., Mar 17 2021, In: Journal of the American Chemical Society. 143, 10, p. 3729-3733 5 p.

Research output: Contribution to journal › Article › peer-review

Open Access

2020

Antibody Targeted PET Imaging of ⁶⁴Cu-DOTA-Anti-CEA PEGylated Lipid Nanodiscs in CEA Positive Tumors

Wong, P., Li, L., Chea, J., Hu, W., Poku, E., Ebner, T., Bowles, N., Wong, J. Y. C., Yazaki, P. J., Sligar, S. & Shively, J. E., Mar 18 2020, In: Bioconjugate Chemistry. 31, 3, p. 743-753 11 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Dark, Ultra-Dark and Ultra-Bright Nanodiscs for membrane protein investigations

McLean, M. A., Denisov, I. G., Grinkova, Y. V. & Sligar, S. G., Oct 15 2020, In: Analytical Biochemistry. 607, 113860.

Research output: Contribution to journal › Article › peer-review

Open Access

Molecular Orientation Determination in Nanodiscs at the Single-Molecule Level

Camp, T., Mehta, K., Sligar, S. G. & Zhang, K., Jan 21 2020, In: Analytical Chemistry. 92, 2, p. 2229-2236 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Nanodisc self-assembly is thermodynamically reversible and controllable

Camp, T., Sligar, S. G., Sligar, S. G. & Sligar, S. G., Jun 28 2020, In: Soft Matter. 16, 24, p. 5615-5623 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

P450 CYP17A1 Variant with a Disordered Proton Shuttle Assembly Retains Peroxo-Mediated Lyase Efficiency

Liu, Y., Denisov, I. G., Grinkova, Y. V., Sligar, S. G. & Kincaid, J. R., Dec 15 2020, In: Chemistry - A European Journal. 26, 70, p. 16846-16852 7 p.

Research output: Contribution to journal › Article › peer-review

Open Access

2019

Allosteric Interactions in Human Cytochrome P450 CYP3A4: The Role of Phenylalanine 213

Denisov, I. G., Grinkova, Y. V., Nandigrami, P., Shekhar, M., Tajkhorshid, E. & Sligar, S. G., Mar 12 2019, In: Biochemistry. 58, 10, p. 1411-1422 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Biotransformation of the mycotoxin enniatin b1 by CYP P450 3A4 and potential for drug-drug interactions

Ivanova, L., Denisov, I. G., Grinkova, Y. V., Sligar, S. G. & Fæste, C. K., Aug 2019, In: Metabolites. 9, 8, 158.

Research output: Contribution to journal › Article › peer-review

Open Access

Influence of Transmembrane Helix Mutations on Cytochrome P450-Membrane Interactions and Function

Mustafa, G., Nandekar, P. P., Camp, T. J., Bruce, N. J., Gregory, M. C., Sligar, S. G. & Wade, R. C., Feb 5 2019, In: Biophysical journal. 116, 3, p. 419-432 14 p.

Research output: Contribution to journal › Article › peer-review

Open Access

NMR analysis of free and lipid nanodisc anchored CEACAM1 membrane proximal peptides with Ca²⁺/CaM

Ghazarian, H., Hu, W., Mao, A., Nguyen, T., Vaidehi, N., Sligar, S. & Shively, J. E., Apr 1 2019, In: Biochimica et Biophysica Acta - Biomembranes. 1861, 4, p. 787-797 11 p.

Research output: Contribution to journal › Article › peer-review

Open Access

PIP2 Influences the Conformational Dynamics of Membrane-Bound KRAS4b

McLean, M. A., Stephen, A. G. & Sligar, S. G., Aug 20 2019, In: Biochemistry. 58, 33, p. 3537-3545 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

The hydrodynamic motion of Nanodiscs

Camp, T., McLean, M., Kato, M., Cheruzel, L. & Sligar, S., May 2019, In: Chemistry and Physics of Lipids. 220, p. 28-35 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

2018

Cytochrome b₅ enhances androgen synthesis by rapidly reducing the CYP17A1 oxy-complex in the lyase step

Duggal, R., Denisov, I. G. & Sligar, S. G., Jul 2018, In: FEBS Letters. 592, 13, p. 2282-2288 7 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Drug-Drug Interactions between Atorvastatin and Dronedarone Mediated by Monomeric CYP3A4

Denisov, I. G., Baylon, J. L., Grinkova, Y. V., Tajkhorshid, E. & Sligar, S. G., Feb 6 2018, In: Biochemistry. 57, 5, p. 805-816 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Human Cytochrome CYP17A1: The Structural Basis for Compromised Lyase Activity with 17-Hydroxyprogesterone

Mak, P. J., Duggal, R., Denisov, I. G., Gregory, M. C., Sligar, S. G. & Kincaid, J. R., Jun 13 2018, In: Journal of the American Chemical Society. 140, 23, p. 7324-7331 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Human P450 CYP17A1: Control of Substrate Preference by Asparagine 202

Gregory, M. C., Mak, P. J., Khatri, Y., Kincaid, J. R. & Sligar, S. G., Feb 6 2018, In: Biochemistry. 57, 5, p. 764-771 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

2017

Alzheimer’s toxic amyloid beta oligomers: Unwelcome visitors to the Na/K ATPase alpha3 docking station

di Chiara, T., DiNunno, N., Clark, J., Bu, R. L., Cline, E. N., Rollins, M. G., Gong, Y., Brody, D. L., Sligar, S. G., Velasco, P. T., Viola, K. L. & Klein, W. L., Mar 2017, In: Yale Journal of Biology and Medicine. 90, 1, p. 45-61 17 p.

Research output: Contribution to journal › Article › peer-review

Conformational equilibria of light-activated rhodopsin in nanodiscs

Van Eps, N., Caro, L. N., Morizumi, T., Kusnetzow, A. K., Szczepek, M., Hofmann, K. P., Bayburt, T. H., Sligar, S. G., Ernst, O. P. & Hubbell, W. L., Apr 18 2017, In: Proceedings of the National Academy of Sciences of the United States of America. 114, 16, p. E3268-E3275

Research output: Contribution to journal › Article › peer-review

Open Access

Heme Binding Biguanides Target Cytochrome P450-Dependent Cancer Cell Mitochondria

Guo, Z., Sevrioukova, I. F., Denisov, I. G., Zhang, X., Chiu, T. L., Thomas, D. G., Hanse, E. A., Cuellar, R. A. D., Grinkova, Y. V., Langenfeld, V. W., Swedien, D. S., Stamschror, J. D., Alvarez, J., Luna, F., Galván, A., Bae, Y. K., Wulfkuhle, J. D., Gallagher, R. I., Petricoin, E. F. & Norris, B. & 17 others, Flory, C. M., Schumacher, R. J., O'Sullivan, M. G., Cao, Q., Chu, H., Lipscomb, J. D., Atkins, W. M., Gupta, K., Kelekar, A., Blair, I. A., Capdevila, J. H., Falck, J. R., Sligar, S. G., Poulos, T. L., Georg, G. I., Ambrose, E. & Potter, D. A., Oct 19 2017, In: Cell chemical biology. 24, 10, p. 1259-1275.e6

Research output: Contribution to journal › Article › peer-review

Open Access

Interaction of KRas4b with anionic membranes: A special role for PIP₂

Gregory, M. C., McLean, M. A. & Sligar, S. G., May 27 2017, In: Biochemical and Biophysical Research Communications. 487, 2, p. 351-355 5 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Microfluidic platform for efficient Nanodisc assembly, membrane protein incorporation, and purification

Wade, J. H., Jones, J. D., Lenov, I. L., Riordan, C. M., Sligar, S. G. & Bailey, R. C., Sep 7 2017, In: Lab on a chip. 17, 17, p. 2951-2959 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

2016

Conformational equilibrium of talin is regulated by anionic lipids

Ye, X., McLean, M. A. & Sligar, S. G., Aug 1 2016, In: Biochimica et Biophysica Acta - Biomembranes. 1858, 8, p. 1833-1840 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Evidence that cytochrome b₅ acts as a redox donor in CYP17A1 mediated androgen synthesis

Duggal, R., Liu, Y., Gregory, M. C., Denisov, I. G., Kincaid, J. R. & Sligar, S. G., Aug 19 2016, In: Biochemical and Biophysical Research Communications. 477, 2, p. 202-208 7 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Phosphatidylinositol 4,5-Bisphosphate Modulates the Affinity of Talin-1 for Phospholipid Bilayers and Activates Its Autoinhibited Form

Ye, X., McLean, M. A. & Sligar, S. G., Sep 13 2016, In: Biochemistry. 55, 36, p. 5038-5048 11 p.

Research output: Contribution to journal › Article › peer-review

Open Access

The Charge Properties of Phospholipid Nanodiscs

Her, C., Filoti, D. I., McLean, M. A., Sligar, S. G., Alexander Ross, J. B., Steele, H. & Laue, T. M., Sep 6 2016, In: Biophysical journal. 111, 5, p. 989-998 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

The use of isomeric testosterone dimers to explore allosteric effects in substrate binding to cytochrome P450 CYP3A4

Denisov, I. G., Mak, P. J., Grinkova, Y. V., Bastien, D., Bérubé, G., Sligar, S. G. & Kincaid, J. R., May 1 2016, In: Journal of Inorganic Biochemistry. 158, p. 77-85 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Trimerization of the HIV Transmembrane Domain in Lipid Bilayers Modulates Broadly Neutralizing Antibody Binding

Reichart, T. M., Baksh, M. M., Rhee, J. K., Fiedler, J. D., Sligar, S. G., Finn, M. G., Zwick, M. B. & Dawson, P. E., Feb 18 2016, In: Angewandte Chemie - International Edition. 55, 8, p. 2688-2692 5 p.

Research output: Contribution to journal › Article › peer-review

Open Access

2015

Mechanism of Drug-Drug Interactions Mediated by Human Cytochrome P450 CYP3A4 Monomer

Denisov, I. G., Grinkova, Y. V., Baylon, J. L., Tajkhorshid, E. & Sligar, S. G., Apr 7 2015, In: Biochemistry. 54, 13, p. 2227-2239 13 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Nanodiscs as a modular platform for multimodal MR-optical imaging

Carney, C. E., Lenov, I. L., Baker, C. J., Macrenaris, K. W., Eckermann, A. L., Sligar, S. G. & Meade, T. J., May 20 2015, In: Bioconjugate Chemistry. 26, 5, p. 899-905 7 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Nanoscale synaptic membrane mimetic allows unbiased high throughput screen that targets binding sites for Alzheimer's-associated Aβ oligomers

Wilcox, K. C., Marunde, M. R., Das, A., Velasco, P. T., Kuhns, B. D., Marty, M. T., Jiang, H., Luan, C. H., Sligar, S. G. & Klein, W. L., Apr 1 2015, In: PloS one. 10, 4, e0125263.

Research output: Contribution to journal › Article › peer-review

Open Access

Small-angle scattering determination of the shape and localization of human cytochrome P450 embedded in a phospholipid nanodisc environment

Skar-Gislinge, N., Kynde, S. A. R., Denisov, I. G., Ye, X., Lenov, I., Sligar, S. G. & Arleth, L., 2015, In: Acta Crystallographica Section D: Biological Crystallography. 71, p. 2412-2421 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Unveiling the crucial intermediates in androgen production

Mak, P. J., Gregory, M. C., Denisov, I. G., Sligar, S. G. & Kincaid, J. R., Dec 29 2015, In: Proceedings of the National Academy of Sciences of the United States of America. 112, 52, p. 15856-15861 6 p.

Research output: Contribution to journal › Article › peer-review

Open Access

2014

Active site proton delivery and the lyase activity of human CYP17A1

Khatri, Y., Gregory, M. C., Grinkova, Y. V., Denisov, I. G. & Sligar, S. G., Jan 3 2014, In: Biochemical and Biophysical Research Communications. 443, 1, p. 179-184 6 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Assembly of an activated rhodopsin-transducin complex in nanoscale lipid bilayers

D'Antona, A. M., Xie, G., Sligar, S. G. & Oprian, D. D., Jan 14 2014, In: Biochemistry. 53, 1, p. 127-134 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Interpretation and deconvolution of nanodisc native mass spectra

Marty, M. T., Zhang, H., Cui, W., Gross, M. L. & Sligar, S. G., Feb 2014, In: Journal of the American Society for Mass Spectrometry. 25, 2, p. 269-277 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Kinetic solvent isotope effect in steady-state turnover by CYP19A1 suggests involvement of Compound 1 for both hydroxylation and aromatization steps

Khatri, Y., Luthra, A., Duggal, R. & Sligar, S. G., Aug 25 2014, In: FEBS Letters. 588, 17, p. 3117-3122 6 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Resonance raman spectroscopy of the oxygenated intermediates of human CYP19A1 implicates a compound i intermediate in the final lyase step

Mak, P. J., Luthra, A., Sligar, S. G. & Kincaid, J. R., Apr 2 2014, In: Journal of the American Chemical Society. 136, 13, p. 4825-4828 4 p.

Research output: Contribution to journal › Article › peer-review

Resonance raman spectroscopy reveals that substrate structure selectively impacts the heme-bound diatomic Ligands of CYP17

Mak, P. J., Gregory, M. C., Sligar, S. G. & Kincaid, J. R., Jan 14 2014, In: Biochemistry. 53, 1, p. 90-100 11 p.

Research output: Contribution to journal › Article › peer-review

Open Access

2013

Characterizing the membrane-bound state of cytochrome P450 3A4: Structure, depth of insertion, and orientation

Baylon, J. L., Lenov, I. L., Sligar, S. G. & Tajkhorshid, E., Jun 12 2013, In: Journal of the American Chemical Society. 135, 23, p. 8542-8551 10 p.

Research output: Contribution to journal › Article › peer-review

Stephen G Sligar

Research output

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