Stephen G Sligar

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Research Output

2020

Antibody Targeted PET Imaging of 64Cu-DOTA-Anti-CEA PEGylated Lipid Nanodiscs in CEA Positive Tumors

Wong, P., Li, L., Chea, J., Hu, W., Poku, E., Ebner, T., Bowles, N., Wong, J. Y. C., Yazaki, P. J., Sligar, S. & Shively, J. E., Mar 18 2020, In : Bioconjugate Chemistry. 31, 3, p. 743-753 11 p.

Research output: Contribution to journalArticle

Membrane-Bound Ras as a Conformational Clock

Gorfe, A. A. & Sligar, S. G., Jan 1 2020, (Accepted/In press) In : Biophysical journal.

Research output: Contribution to journalArticle

Molecular Orientation Determination in Nanodiscs at the Single-Molecule Level

Camp, T., Mehta, K., Sligar, S. G. & Zhang, K., Jan 21 2020, In : Analytical chemistry. 92, 2, p. 2229-2236 8 p.

Research output: Contribution to journalArticle

Nanodisc self-assembly is thermodynamically reversible and controllable

Camp, T., Sligar, S. G., Sligar, S. G. & Sligar, S. G., Jun 28 2020, In : Soft Matter. 16, 24, p. 5615-5623 9 p.

Research output: Contribution to journalArticle

2019

Allosteric Interactions in Human Cytochrome P450 CYP3A4: The Role of Phenylalanine 213

Denisov, I. G., Grinkova, Y. V., Nandigrami, P., Shekhar, M., Tajkhorshid, E. & Sligar, S. G., Mar 12 2019, In : Biochemistry. 58, 10, p. 1411-1422 12 p.

Research output: Contribution to journalArticle

Biotransformation of the mycotoxin enniatin b1 by CYP P450 3A4 and potential for drug-drug interactions

Ivanova, L., Denisov, I. G., Grinkova, Y. V., Sligar, S. G. & Fæste, C. K., Aug 2019, In : Metabolites. 9, 8, 158.

Research output: Contribution to journalArticle

Open Access

Influence of Transmembrane Helix Mutations on Cytochrome P450-Membrane Interactions and Function

Mustafa, G., Nandekar, P. P., Camp, T. J., Bruce, N. J., Gregory, M. C., Sligar, S. G. & Wade, R. C., Feb 5 2019, In : Biophysical journal. 116, 3, p. 419-432 14 p.

Research output: Contribution to journalArticle

Nanodiscs as a New Tool to Examine Lipid–Protein Interactions

Denisov, I. G., Schuler, M. A. & Sligar, S. G., Jan 1 2019, Methods in Molecular Biology. Humana Press Inc., p. 645-671 27 p. (Methods in Molecular Biology; vol. 2003).

Research output: Chapter in Book/Report/Conference proceedingChapter

NMR analysis of free and lipid nanodisc anchored CEACAM1 membrane proximal peptides with Ca 2+ /CaM

Ghazarian, H., Hu, W., Mao, A., Nguyen, T., Vaidehi, N., Sligar, S. & Shively, J. E., Apr 1 2019, In : Biochimica et Biophysica Acta - Biomembranes. 1861, 4, p. 787-797 11 p.

Research output: Contribution to journalArticle

PIP2 Influences the Conformational Dynamics of Membrane-Bound KRAS4b

McLean, M. A., Stephen, A. G. & Sligar, S. G., Aug 20 2019, In : Biochemistry. 58, 33, p. 3537-3545 9 p.

Research output: Contribution to journalArticle

The hydrodynamic motion of Nanodiscs

Camp, T., McLean, M., Kato, M., Cheruzel, L. & Sligar, S., May 2019, In : Chemistry and Physics of Lipids. 220, p. 28-35 8 p.

Research output: Contribution to journalArticle

2018

Cytochrome b5 enhances androgen synthesis by rapidly reducing the CYP17A1 oxy-complex in the lyase step

Duggal, R., Denisov, I. G. & Sligar, S. G., Jul 2018, In : FEBS Letters. 592, 13, p. 2282-2288 7 p.

Research output: Contribution to journalLetter

Drug-Drug Interactions between Atorvastatin and Dronedarone Mediated by Monomeric CYP3A4

Denisov, I. G., Baylon, J. L., Grinkova, Y. V., Tajkhorshid, E. & Sligar, S. G., Feb 6 2018, In : Biochemistry. 57, 5, p. 805-816 12 p.

Research output: Contribution to journalArticle

Human Cytochrome CYP17A1: The Structural Basis for Compromised Lyase Activity with 17-Hydroxyprogesterone

Mak, P. J., Duggal, R., Denisov, I. G., Gregory, M. C., Sligar, S. G. & Kincaid, J. R., Jun 13 2018, In : Journal of the American Chemical Society. 140, 23, p. 7324-7331 8 p.

Research output: Contribution to journalArticle

Human P450 CYP17A1: Control of Substrate Preference by Asparagine 202

Gregory, M. C., Mak, P. J., Khatri, Y., Kincaid, J. R. & Sligar, S. G., Feb 6 2018, In : Biochemistry. 57, 5, p. 764-771 8 p.

Research output: Contribution to journalArticle

Nanodiscs: A Controlled Bilayer Surface for the Study of Membrane Proteins

McLean, M. A., Gregory, M. C. & Sligar, S. G., May 20 2018, In : Annual Review of Biophysics. 47, p. 107-124 18 p.

Research output: Contribution to journalReview article

SMPL synaptic membranes: Nanodisc-mediated synaptic membrane mimetics expand the toolkit for drug discovery and the molecular cell biology of synapses

Lo Bu, R., Clark, J., DiChiara, T., Sligar, S. G. & Klein, W. L., Jan 1 2018, Neuromethods. Humana Press Inc., p. 227-250 24 p. (Neuromethods; vol. 141).

Research output: Chapter in Book/Report/Conference proceedingChapter

2017

Alzheimer’s toxic amyloid beta oligomers: Unwelcome visitors to the Na/K ATPase alpha3 docking station

di Chiara, T., DiNunno, N., Clark, J., Bu, R. L., Cline, E. N., Rollins, M. G., Gong, Y., Brody, D. L., Sligar, S. G., Velasco, P. T., Viola, K. L. & Klein, W. L., Mar 2017, In : Yale Journal of Biology and Medicine. 90, 1, p. 45-61 17 p.

Research output: Contribution to journalArticle

Conformational equilibria of light-activated rhodopsin in nanodiscs

Van Eps, N., Caro, L. N., Morizumi, T., Kusnetzow, A. K., Szczepek, M., Hofmann, K. P., Bayburt, T. H., Sligar, S. G., Ernst, O. P. & Hubbell, W. L., Apr 18 2017, In : Proceedings of the National Academy of Sciences of the United States of America. 114, 16, p. E3268-E3275

Research output: Contribution to journalArticle

Heme Binding Biguanides Target Cytochrome P450-Dependent Cancer Cell Mitochondria

Guo, Z., Sevrioukova, I. F., Denisov, I. G., Zhang, X., Chiu, T. L., Thomas, D. G., Hanse, E. A., Cuellar, R. A. D., Grinkova, Y. V., Langenfeld, V. W., Swedien, D. S., Stamschror, J. D., Alvarez, J., Luna, F., Galván, A., Bae, Y. K., Wulfkuhle, J. D., Gallagher, R. I., Petricoin, E. F., Norris, B. & 17 others, Flory, C. M., Schumacher, R. J., O'Sullivan, M. G., Cao, Q., Chu, H., Lipscomb, J. D., Atkins, W. M., Gupta, K., Kelekar, A., Blair, I. A., Capdevila, J. H., Falck, J. R., Sligar, S. G., Poulos, T. L., Georg, G. I., Ambrose, E. & Potter, D. A., Oct 19 2017, In : Cell chemical biology. 24, 10, p. 1259-1275.e6

Research output: Contribution to journalArticle

Interaction of KRas4b with anionic membranes: A special role for PIP2

Gregory, M. C., McLean, M. A. & Sligar, S. G., May 27 2017, In : Biochemical and Biophysical Research Communications. 487, 2, p. 351-355 5 p.

Research output: Contribution to journalArticle

Microfluidic platform for efficient Nanodisc assembly, membrane protein incorporation, and purification

Wade, J. H., Jones, J. D., Lenov, I. L., Riordan, C. M., Sligar, S. G. & Bailey, R. C., Sep 7 2017, In : Lab on a chip. 17, 17, p. 2951-2959 9 p.

Research output: Contribution to journalArticle

Nanodiscs in Membrane Biochemistry and Biophysics

Denisov, I. G. & Sligar, S. G., Mar 22 2017, In : Chemical reviews. 117, 6, p. 4669-4713 45 p.

Research output: Contribution to journalReview article

2016

Conformational equilibrium of talin is regulated by anionic lipids

Ye, X., McLean, M. A. & Sligar, S. G., Aug 1 2016, In : Biochimica et Biophysica Acta - Biomembranes. 1858, 8, p. 1833-1840 8 p.

Research output: Contribution to journalArticle

Evidence that cytochrome b5 acts as a redox donor in CYP17A1 mediated androgen synthesis

Duggal, R., Liu, Y., Gregory, M. C., Denisov, I. G., Kincaid, J. R. & Sligar, S. G., Aug 19 2016, In : Biochemical and Biophysical Research Communications. 477, 2, p. 202-208 7 p.

Research output: Contribution to journalArticle

Nanodiscs for structural and functional studies of membrane proteins

Denisov, I. G. & Sligar, S. G., Jun 7 2016, In : Nature Structural and Molecular Biology. 23, 6, p. 481-486 6 p.

Research output: Contribution to journalReview article

Phosphatidylinositol 4,5-Bisphosphate Modulates the Affinity of Talin-1 for Phospholipid Bilayers and Activates Its Autoinhibited Form

Ye, X., McLean, M. A. & Sligar, S. G., Sep 13 2016, In : Biochemistry. 55, 36, p. 5038-5048 11 p.

Research output: Contribution to journalArticle

The Charge Properties of Phospholipid Nanodiscs

Her, C., Filoti, D. I., McLean, M. A., Sligar, S. G., Alexander Ross, J. B., Steele, H. & Laue, T. M., Sep 6 2016, In : Biophysical journal. 111, 5, p. 989-998 10 p.

Research output: Contribution to journalArticle

The use of isomeric testosterone dimers to explore allosteric effects in substrate binding to cytochrome P450 CYP3A4

Denisov, I. G., Mak, P. J., Grinkova, Y. V., Bastien, D., Bérubé, G., Sligar, S. G. & Kincaid, J. R., May 1 2016, In : Journal of Inorganic Biochemistry. 158, p. 77-85 9 p.

Research output: Contribution to journalArticle

Trimerization of the HIV Transmembrane Domain in Lipid Bilayers Modulates Broadly Neutralizing Antibody Binding

Reichart, T. M., Baksh, M. M., Rhee, J. K., Fiedler, J. D., Sligar, S. G., Finn, M. G., Zwick, M. B. & Dawson, P. E., Feb 18 2016, In : Angewandte Chemie - International Edition. 55, 8, p. 2688-2692 5 p.

Research output: Contribution to journalArticle

2015

Activation of molecular oxygen in cytochromes P450

Denisov, I. G. & Sligar, S. G., Jan 1 2015, Cytochrome P450: Structure, Mechanism, and Biochemistry, Fourth Edition. Springer International Publishing, p. 69-109 41 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Mechanism of Drug-Drug Interactions Mediated by Human Cytochrome P450 CYP3A4 Monomer

Denisov, I. G., Grinkova, Y. V., Baylon, J. L., Tajkhorshid, E. & Sligar, S. G., Apr 7 2015, In : Biochemistry. 54, 13, p. 2227-2239 13 p.

Research output: Contribution to journalArticle

Metalloproteins: The long and the short of it

Denisov, I. G. & Sligar, S. G., Aug 20 2015, In : Nature Chemistry. 7, 9, p. 687-688 2 p.

Research output: Contribution to journalShort survey

Nanodiscs as a modular platform for multimodal MR-optical imaging

Carney, C. E., Lenov, I. L., Baker, C. J., Macrenaris, K. W., Eckermann, A. L., Sligar, S. G. & Meade, T. J., May 20 2015, In : Bioconjugate Chemistry. 26, 5, p. 899-905 7 p.

Research output: Contribution to journalArticle

Nanoscale synaptic membrane mimetic allows unbiased high throughput screen that targets binding sites for Alzheimer's-associated Aβ oligomers

Wilcox, K. C., Marunde, M. R., Das, A., Velasco, P. T., Kuhns, B. D., Marty, M. T., Jiang, H., Luan, C. H., Sligar, S. G. & Klein, W. L., Apr 1 2015, In : PloS one. 10, 4, e0125263.

Research output: Contribution to journalArticle

Small-angle scattering determination of the shape and localization of human cytochrome P450 embedded in a phospholipid nanodisc environment

Skar-Gislinge, N., Kynde, S. A. R., Denisov, I. G., Ye, X., Lenov, I., Sligar, S. G. & Arleth, L., Jan 1 2015, In : Acta Crystallographica Section D: Biological Crystallography. 71, p. 2412-2421 10 p.

Research output: Contribution to journalArticle

Unveiling the crucial intermediates in androgen production

Mak, P. J., Gregory, M. C., Denisov, I. G., Sligar, S. G. & Kincaid, J. R., Dec 29 2015, In : Proceedings of the National Academy of Sciences of the United States of America. 112, 52, p. 15856-15861 6 p.

Research output: Contribution to journalArticle

2014

Active site proton delivery and the lyase activity of human CYP17A1

Khatri, Y., Gregory, M. C., Grinkova, Y. V., Denisov, I. G. & Sligar, S. G., Jan 3 2014, In : Biochemical and Biophysical Research Communications. 443, 1, p. 179-184 6 p.

Research output: Contribution to journalArticle

Assembly of an activated rhodopsin-transducin complex in nanoscale lipid bilayers

D'Antona, A. M., Xie, G., Sligar, S. G. & Oprian, D. D., Jan 14 2014, In : Biochemistry. 53, 1, p. 127-134 8 p.

Research output: Contribution to journalArticle

Interpretation and deconvolution of nanodisc native mass spectra

Marty, M. T., Zhang, H., Cui, W., Gross, M. L. & Sligar, S. G., Feb 1 2014, In : Journal of the American Society for Mass Spectrometry. 25, 2, p. 269-277 9 p.

Research output: Contribution to journalArticle

Kinetic solvent isotope effect in steady-state turnover by CYP19A1 suggests involvement of Compound 1 for both hydroxylation and aromatization steps

Khatri, Y., Luthra, A., Duggal, R. & Sligar, S. G., Aug 25 2014, In : FEBS Letters. 588, 17, p. 3117-3122 6 p.

Research output: Contribution to journalArticle

Resonance raman spectroscopy of the oxygenated intermediates of human CYP19A1 implicates a compound i intermediate in the final lyase step

Mak, P. J., Luthra, A., Sligar, S. G. & Kincaid, J. R., Apr 2 2014, In : Journal of the American Chemical Society. 136, 13, p. 4825-4828 4 p.

Research output: Contribution to journalArticle

Resonance raman spectroscopy reveals that substrate structure selectively impacts the heme-bound diatomic Ligands of CYP17

Mak, P. J., Gregory, M. C., Sligar, S. G. & Kincaid, J. R., Jan 14 2014, In : Biochemistry. 53, 1, p. 90-100 11 p.

Research output: Contribution to journalArticle

2013

Characterizing the membrane-bound state of cytochrome P450 3A4: Structure, depth of insertion, and orientation

Baylon, J. L., Lenov, I. L., Sligar, S. G. & Tajkhorshid, E., Jun 12 2013, In : Journal of the American Chemical Society. 135, 23, p. 8542-8551 10 p.

Research output: Contribution to journalArticle

Constitutively active rhodopsin mutants causing night blindness are effectively phosphorylated by GRKs but differ in arrestin-1 binding

Vishnivetskiy, S. A., Ostermaier, M. K., Singhal, A., Panneels, V., Homan, K. T., Glukhova, A., Sligar, S. G., Tesmer, J. J. G., Schertler, G. F. X., Standfuss, J. & Gurevich, V. V., Nov 1 2013, In : Cellular Signalling. 25, 11, p. 2155-2162 8 p.

Research output: Contribution to journalArticle

Differential hydrogen bonding in human CYP17 dictates hydroxylation versus lyase chemistry

Gregory, M., Mak, P. J., Sligar, S. G. & Kincaid, J. R., May 10 2013, In : Angewandte Chemie - International Edition. 52, 20, p. 5342-5345 4 p.

Research output: Contribution to journalArticle

Interfacing lipid bilayer nanodiscs and silicon photonic sensor arrays for multiplexed protein-lipid and protein-membrane protein interaction screening

Sloan, C. D. K., Marty, M. T., Sligar, S. G. & Bailey, R. C., Mar 5 2013, In : Analytical chemistry. 85, 5, p. 2970-2976 7 p.

Research output: Contribution to journalArticle

Investigations of heme ligation and ligand switching in cytochromes P450 and P420

Sun, Y., Zeng, W., Benabbas, A., Ye, X., Denisov, I., Sligar, S. G., Du, J., Dawson, J. H. & Champion, P. M., Aug 27 2013, In : Biochemistry. 52, 34, p. 5941-5951 11 p.

Research output: Contribution to journalArticle

Kinetic solvent isotope effect in human P450 CYP17A1-mediated androgen formation: Evidence for a reactive peroxoanion intermediate

Gregory, M. C., Denisov, I. G., Grinkova, Y. V., Khatri, Y. & Sligar, S. G., Nov 6 2013, In : Journal of the American Chemical Society. 135, 44, p. 16245-16247 3 p.

Research output: Contribution to journalArticle

Nanodiscs as a new tool to examine lipid-protein interactions

Schuler, M. A., Denisov, I. G. & Sligar, S. G., 2013, Lipid-Protein Interactions: Methods and Protocols. Humana Press Inc., p. 415-433 19 p. (Methods in Molecular Biology; vol. 974).

Research output: Chapter in Book/Report/Conference proceedingChapter