Robert Bennett Gennis

2024

Diversity and evolution of nitric oxide reduction in bacteria and archaea

Murali, R., Pace, L. A., Sanford, R. A., Ward, L. M., Lynes, M. M., Hatzenpichler, R., Lingappa, U. F., Fischer, W. W., Gennis, R. B. & Hemp, J., Jun 1 2024, In: Proceedings of the National Academy of Sciences of the United States of America. 121, 26, e2316422121.

Research output: Contribution to journal › Article › peer-review

Open Access

2023

Structures of the intermediates in the catalytic cycle of mitochondrial cytochrome c oxidase

Wikström, M., Gennis, R. B. & Rich, P. R., Apr 1 2023, In: Biochimica et Biophysica Acta - Bioenergetics. 1864, 2, 148933.

Research output: Contribution to journal › Review article › peer-review

Open Access

2022

Evolution of quinol oxidation within the heme‑copper oxidoreductase superfamily

Murali, R., Hemp, J. & Gennis, R. B., Nov 1 2022, In: Biochimica et Biophysica Acta - Bioenergetics. 1863, 8, 148907.

Research output: Contribution to journal › Article › peer-review

Open Access

Ligand binding and conformational dynamics of the E. coli nicotinamide nucleotide transhydrogenase revealed by hydrogen/deuterium exchange mass spectrometry

Zöller, J., Hong, S., Eisinger, M. L., Anderson, M., Radloff, M., Desch, K., Gennis, R. & Langer, J. D., Jan 2022, In: Computational and Structural Biotechnology Journal. 20, p. 5430-5439 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

2021

Cryo-EM structures of Escherichia coli cytochrome bo₃ reveal bound phospholipids and ubiquinone-8 in a dynamic substrate binding site

Li, J., Han, L., Vallese, F., Ding, Z., Choi, S. K., Hong, S., Luo, Y., Liu, B., Chan, C. K., Tajkhorshid, E., Zhu, J., Clarke, O., Zhang, K. & Gennis, R., Aug 24 2021, In: Proceedings of the National Academy of Sciences of the United States of America. 118, 34, e2106750118.

Research output: Contribution to journal › Article › peer-review

Energy transfer between the nicotinamide nucleotide transhydrogenase and ATP synthase of Escherichia coli

Graf, S. S., Hong, S., Müller, P., Gennis, R. & von Ballmoos, C., Dec 2021, In: Scientific reports. 11, 1, 21234.

Research output: Contribution to journal › Article › peer-review

Open Access

Escherichia coli amino acid auxotrophic expression host strains for investigating protein structure-function relationships

Iwasaki, T., Miyajima-Nakano, Y., Fukazawa, R., Lin, M. T., Matsushita, S.-I., Hagiuda, E., Taguchi, A. T., Dikanov, S. A., Oishi, Y. & Gennis, R. B., Apr 1 2021, In: Journal of Biochemistry. 169, 4, p. 387-394 8 p.

Research output: Contribution to journal › Review article › peer-review

Evolution of the cytochrome bd oxygen reductase superfamily and the function of CydAA’ in Archaea

Murali, R., Gennis, R. B. & Hemp, J., Dec 2021, In: ISME Journal. 15, 12, p. 3534-3548 15 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Identification of a cytochrome bc₁-aa₃ supercomplex in Rhodobacter sphaeroides

Fedotovskaya, O., Albertsson, I., Nordlund, G., Hong, S., Gennis, R. B., Brzezinski, P. & Ädelroth, P., Aug 1 2021, In: Biochimica et Biophysica Acta - Bioenergetics. 1862, 8, 148433.

Research output: Contribution to journal › Article › peer-review

Open Access

Specific inhibition of proton pumping by the T315V mutation in the K channel of cytochrome ba₃ from Thermus thermophilus

Siletsky, S. A., Soulimane, T., Belevich, I., Gennis, R. B. & Wikström, M., Sep 1 2021, In: Biochimica et Biophysica Acta - Bioenergetics. 1862, 9, 148450.

Research output: Contribution to journal › Article › peer-review

Open Access

The Monoheme c Subunit of Respiratory Alternative Complex III Is Not Essential for Electron Transfer to Cytochrome aa₃ in Flavobacterium johnsoniae

Lorencik, K., Ekiert, R., Zhu, Y., McBride, M. J., Gennis, R. B., Sarewicz, M. & Osyczka, A., Sep 2021, In: Microbiology Spectrum. 9, 1, p. 1-12 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

The three-spin intermediate at the O–O cleavage and proton-pumping junction in heme–Cu oxidases

Jose, A., Schaefer, A. W., Roveda, A. C., Transue, W. J., Choi, S. K., Ding, Z., Gennis, R. B. & Solomon, E. I., Sep 10 2021, In: Science. 373, 6560, p. 1225-1229 5 p.

Research output: Contribution to journal › Article › peer-review

Time-Resolved Electrometric Study of the F→O Transition in Cytochrome c Oxidase. The Effect of Zn²⁺ Ions on the Positive Side of the Membrane

Siletsky, S. A. & Gennis, R. B., Jan 2021, In: Biochemistry (Moscow). 86, 1, p. 105-122 18 p.

Research output: Contribution to journal › Article › peer-review

2020

Discovery of Prenyltransferase Inhibitors with in Vitro and in Vivo Antibacterial Activity

Song, J., Malwal, S. R., Baig, N., Schurig-Briccio, L. A., Gao, Z., Vaidya, G. S., Yang, K., Abutaleb, N. S., Seleem, M. N., Gennis, R. B., Pogorelov, T. V., Oldfield, E. & Feng, X., Nov 13 2020, In: ACS Infectious Diseases. 6, 11, p. 2979-2993 15 p.

Research output: Contribution to journal › Article › peer-review

Role of respiratory NADH oxidation in the regulation of Staphylococcus aureus virulence

Schurig-Briccio, L. A., Parraga Solorzano, P. K., Lencina, A. M., Radin, J. N., Chen, G. Y., Sauer, J. D., Kehl-Fie, T. E. & Gennis, R. B., May 6 2020, In: EMBO Reports. 21, 5, e45832.

Research output: Contribution to journal › Article › peer-review

Open Access

Structure of the cytochrome aa₃-600 heme-copper menaquinol oxidase bound to inhibitor HQNO shows TM0 is part of the quinol binding site

Xu, J., Ding, Z., Liu, B., Yi, S. M., Li, J., Zhang, Z., Liu, Y., Li, J., Liu, L., Zhou, A., Gennis, R. B. & Zhu, J., Jan 14 2020, In: Proceedings of the National Academy of Sciences of the United States of America. 117, 2, p. 872-876 5 p.

Research output: Contribution to journal › Article › peer-review

Open Access

The carboxy-terminal insert in the Q-loop is needed for functionality of Escherichia coli cytochrome bd-I

Goojani, H. G., Konings, J., Hakvoort, H., Hong, S., Gennis, R. B., Sakamoto, J., Lill, H. & Bald, D., Jun 1 2020, In: Biochimica et Biophysica Acta - Bioenergetics. 1861, 5-6, 148175.

Research output: Contribution to journal › Article › peer-review

Open Access

The oligomeric state of the Caldivirga maquilingensis type III sulfide:Quinone Oxidoreductase is required for membrane binding

Lencina, A. M., Gennis, R. B. & Schurig-Briccio, L. A., Feb 1 2020, In: Biochimica et Biophysica Acta - Bioenergetics. 1861, 2, 148132.

Research output: Contribution to journal › Article › peer-review

Open Access

2019

Active site rearrangement and structural divergence in prokaryotic respiratory oxidases

Safarian, S., Hahn, A., Mills, D. J., Radloff, M., Eisinger, M. L., Nikolaev, A., Meier-Credo, J., Melin, F., Miyoshi, H., Gennis, R. B., Sakamoto, J., Langer, J. D., Hellwig, P., Kühlbrandt, W. & Michel, H., Oct 4 2019, In: Science. 366, 6461, p. 100-104 5 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Characterization and X-ray structure of the NADH-dependent coenzyme A disulfide reductase from Thermus thermophilus

Lencina, A. M., Koepke, J., Preu, J., Muenke, C., Gennis, R. B., Michel, H. & Schurig-Briccio, L. A., Nov 1 2019, In: Biochimica et Biophysica Acta - Bioenergetics. 1860, 11, 148080.

Research output: Contribution to journal › Article › peer-review

Open Access

Microcin J25 inhibits ubiquinol oxidase activity of purified cytochrome bd-I from Escherichia coli

Galván, A. E., Chalón, M. C., Ríos Colombo, N. S., Schurig-Briccio, L. A., Sosa-Padilla, B., Gennis, R. B. & Bellomio, A., May 2019, In: Biochimie. 160, p. 141-147 7 p.

Research output: Contribution to journal › Article › peer-review

Single-particle cryo-EM studies of transmembrane proteins in SMA copolymer nanodiscs

Sun, C. & Gennis, R. B., Jul 2019, In: Chemistry and Physics of Lipids. 221, p. 114-119 6 p.

Research output: Contribution to journal › Review article › peer-review

Open Access

The Ubiquinol Binding Site of Cytochrome bo₃ from Escherichia coli Accommodates Menaquinone and Stabilizes a Functional Menasemiquinone

Ding, Z., Sun, C., Yi, S. M., Gennis, R. B. & Dikanov, S. A., 2019, In: Biochemistry. p. 4559-4569 11 p.

Research output: Contribution to journal › Article › peer-review

2018

Bacterial denitrifying nitric oxide reductases and aerobic respiratory terminal oxidases use similar delivery pathways for their molecular substrates

Mahinthichaichan, P., Gennis, R. B. & Tajkhorshid, E., Sep 2018, In: Biochimica et Biophysica Acta - Bioenergetics. 1859, 9, p. 712-724 13 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Cytochrome aa ₃ Oxygen Reductase Utilizes the Tunnel Observed in the Crystal Structures to Deliver O₂ for Catalysis

Mahinthichaichan, P., Gennis, R. B. & Tajkhorshid, E., Apr 10 2018, In: Biochemistry. 57, 14, p. 2150-2161 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Cytochromes bd-I and bo₃ are essential for the bactericidal effect of microcin J25 on Escherichia coli cells

Galván, A. E., Chalón, M. C., Schurig-Briccio, L. A., Salomón, R. A., Minahk, C. J., Gennis, R. B. & Bellomio, A., Feb 2018, In: Biochimica et Biophysica Acta - Bioenergetics. 1859, 2, p. 110-118 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Functional importance of Glutamate-445 and Glutamate-99 in proton-coupled electron transfer during oxygen reduction by cytochrome bd from Escherichia coli

Murali, R. & Gennis, R. B., Aug 2018, In: Biochimica et Biophysica Acta - Bioenergetics. 1859, 8, p. 577-590 14 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Ionophoric effects of the antitubercular drug bedaquiline

Hards, K., McMillan, D. G. G., Schurig-Briccio, L. A., Gennis, R. B., Lill, H., Bald, D. & Cook, G. M., Jul 10 2018, In: Proceedings of the National Academy of Sciences of the United States of America. 115, 28, p. 7326-7331 6 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Mechanism of proton transfer through the K^C proton pathway in the Vibrio cholerae cbb₃ terminal oxidase

Ahn, Y. O., Albertsson, I., Gennis, R. B. & Ädelroth, P., Nov 2018, In: Biochimica et Biophysica Acta - Bioenergetics. 1859, 11, p. 1191-1198 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Role of the tightly bound quinone for the oxygen reaction of cytochrome bo₃ oxidase from Escherichia coli

Melin, F., Sabuncu, S., Choi, S. K., Leprince, A., Gennis, R. B. & Hellwig, P., Oct 2018, In: FEBS Letters. 592, 20, p. 3380-3387 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Structure of the alternative complex III in a supercomplex with cytochrome oxidase

Sun, C., Benlekbir, S., Venkatakrishnan, P., Wang, Y., Hong, S., Hosler, J., Tajkhorshid, E., Rubinstein, J. L. & Gennis, R. B., May 3 2018, In: Nature. 557, 7703, p. 123-126 4 p.

Research output: Contribution to journal › Article › peer-review

Open Access

The electron distribution in the "activated" state of cytochrome c oxidase

Vilhjálmsdóttir, J., Gennis, R. B. & Brzezinski, P., Dec 1 2018, In: Scientific reports. 8, 1, 7502.

Research output: Contribution to journal › Article › peer-review

Open Access

Type 2 NADH dehydrogenase is the only point of entry for electrons into the Streptococcus agalactiae respiratory chain and is a potential drug target

Lencina, A. M., Franza, T., Sullivan, M. J., Ulett, G. C., Ipe, D. S., Gaudu, P., Gennis, R. B. & Schurig-Briccio, L. A., Jul 1 2018, In: mBio. 9, 4, e01034-18.

Research output: Contribution to journal › Article › peer-review

Open Access

Unpaired Electron Spin Density Distribution across Reduced [2Fe-2S] Cluster Ligands by ¹³C_β-Cysteine Labeling

Taguchi, A. T., Miyajima-Nakano, Y., Fukazawa, R., Lin, M. T., Baldansuren, A., Gennis, R. B., Hasegawa, K., Kumasaka, T., Dikanov, S. A. & Iwasaki, T., Jan 16 2018, In: Inorganic Chemistry. 57, 2, p. 741-746 6 p.

Research output: Contribution to journal › Article › peer-review

X-ray transparent microfluidic platforms for membrane protein crystallization with microseeds

Schieferstein, J. M., Pawate, A. S., Varel, M. J., Guha, S., Astrauskaite, I., Gennis, R. B. & Kenis, P. J. A., Mar 21 2018, In: Lab on a chip. 18, 6, p. 944-954 11 p.

Research output: Contribution to journal › Article › peer-review

Open Access

2017

Critical Role of Water Molecules in Proton Translocation by the Membrane-Bound Transhydrogenase

Padayatti, P. S., Leung, J. H., Mahinthichaichan, P., Tajkhorshid, E., Ishchenko, A., Cherezov, V., Soltis, S. M., Jackson, J. B., Stout, C. D., Gennis, R. B. & Zhang, Q., Jul 5 2017, In: Structure. 25, 7, p. 1111-1119.e3

Research output: Contribution to journal › Article › peer-review

Open Access

Dynamics of the K^B Proton Pathway in Cytochrome ba₃ from Thermus thermophilus

von Ballmoos, C., Smirnova, I., Poiana, F., Gonska, N., Chang, H. Y., Gennis, R. B., Brzezinski, P. & Ädelroth, P., May 1 2017, In: Israel Journal of Chemistry. 57, 5, p. 424-436 13 p.

Research output: Contribution to journal › Article › peer-review

Location of the Substrate Binding Site of the Cytochrome bo₃ Ubiquinol Oxidase from Escherichia coli

Choi, S. K., Schurig-Briccio, L., Ding, Z., Hong, S., Sun, C. & Gennis, R. B., Jun 21 2017, In: Journal of the American Chemical Society. 139, 24, p. 8346-8354 9 p.

Research output: Contribution to journal › Article › peer-review

Searching for the low affinity ubiquinone binding site in cytochrome bo₃ from Escherichia coli

Choi, S. K., Lin, M. T., Ouyang, H. & Gennis, R. B., May 1 2017, In: Biochimica et Biophysica Acta - Bioenergetics. 1858, 5, p. 366-370 5 p.

Research output: Contribution to journal › Article › peer-review

Open Access

X-ray transparent microfluidic chips for high-throughput screening and optimization of in meso membrane protein crystallization

Schieferstein, J. M., Pawate, A. S., Sun, C., Wan, F., Sheraden, P. N., Broecker, J., Ernst, O. P., Gennis, R. B. & Kenis, P. J. A., Mar 1 2017, In: Biomicrofluidics. 11, 2, 024118.

Research output: Contribution to journal › Article › peer-review

Open Access

2016

All the O₂ Consumed by Thermus thermophilus Cytochrome ba₃ Is Delivered to the Active Site through a Long, Open Hydrophobic Tunnel with Entrances within the Lipid Bilayer

Mahinthichaichan, P., Gennis, R. B. & Tajkhorshid, E., Mar 1 2016, In: Biochemistry. 55, 8, p. 1265-1278 14 p.

Research output: Contribution to journal › Article › peer-review

Open Access

A Purple Cupredoxin from Nitrosopumilus maritimus Containing a Mononuclear Type 1 Copper Center with an Open Binding Site

Hosseinzadeh, P., Tian, S., Marshall, N. M., Hemp, J., Mullen, T., Nilges, M. J., Gao, Y. G., Robinson, H., Stahl, D. A., Gennis, R. B. & Lu, Y., May 25 2016, In: Journal of the American Chemical Society. 138, 20, p. 6324-6327 4 p.

Research output: Contribution to journal › Article › peer-review

Open Access

CtaM is required for menaquinol oxidase aa₃ function in Staphylococcus aureus

Hammer, N. D., Schurig-Briccio, L. A., Gerdes, S. Y., Gennis, R. B. & Skaar, E. P., Jul 1 2016, In: mBio. 7, 4, e00823-16.

Research output: Contribution to journal › Article › peer-review

Open Access

Proton Dynamics at the Membrane Surface

Gennis, R. B., May 10 2016, In: Biophysical journal. 110, 9, p. 1909-1911 3 p.

Research output: Contribution to journal › Short survey › peer-review

Open Access

Q-Band Electron-Nuclear Double Resonance Reveals Out-of-Plane Hydrogen Bonds Stabilize an Anionic Ubisemiquinone in Cytochrome bo₃ from Escherichia coli

Sun, C., Taguchi, A. T., Vermaas, J. V., Beal, N. J., O'Malley, P. J., Tajkhorshid, E., Gennis, R. B. & Dikanov, S. A., Oct 11 2016, In: Biochemistry. 55, 40, p. 5714-5725 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

The CO Photodissociation and Recombination Dynamics of the W172Y/F282T Ligand Channel Mutant of Rhodobacter sphaeroides aa₃ Cytochrome c Oxidase

Cassano, J. A., Choi, S. K., McDonald, W., Szundi, I., Villa Gawboy, T. R., Gennis, R. B. & Einarsdóttir, Ó., May 1 2016, In: Photochemistry and Photobiology. 92, 3, p. 410-419 10 p.

Research output: Contribution to journal › Article › peer-review

The unusual redox properties of C-type oxidases

Melin, F., Xie, H., Meyer, T., Ahn, Y. O., Gennis, R. B., Michel, H. & Hellwig, P., Dec 1 2016, In: Biochimica et Biophysica Acta - Bioenergetics. 1857, 12, p. 1892-1899 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

2015

Antiinfectives targeting enzymes and the proton motive force

Feng, X., Zhu, W., Schurig-Briccio, L. A., Lindert, S., Shoen, C., Hitchings, R., Li, J., Wang, Y., Baig, N., Zhou, T., Kim, B. K., Crick, D. C., Cynamon, M., McCammon, J. A., Gennis, R. B. & Oldfield, E., Dec 22 2015, In: Proceedings of the National Academy of Sciences of the United States of America. 112, 51, p. E7073-E7082

Research output: Contribution to journal › Article › peer-review

Open Access

Division of labor in transhydrogenase by alternating proton translocation and hydride transfer

Leung, J. H., Schurig-Briccio, L. A., Yamaguchi, M., Moeller, A., Speir, J. A., Gennis, R. B. & Stout, C. D., Jan 9 2015, In: Science. 347, 6218, p. 178-181 4 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Escherichia coli Auxotroph Host Strains for Amino Acid-Selective Isotope Labeling of Recombinant Proteins

Lin, M. T., Fukazawa, R., Miyajima-Nakano, Y., Matsushita, S., Choi, S. K., Iwasaki, T. & Gennis, R. B., 2015, Methods in Enzymology. Academic Press Inc., p. 45-66 22 p. (Methods in Enzymology; vol. 565).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Robert Bennett Gennis

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