Nicholas Ching Hai Wu

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Research Output

2020

A high-affinity antibody against the CSP N-terminal domain lacks Plasmodium falciparum inhibitory activity

Thai, E., Costa, G., Weyrich, A., Murugan, R., Oyen, D., Flores-Garcia, Y., Prieto, K., Bosch, A., Valleriani, A., Wu, N. C., Pholcharee, T., Scally, S. W., Wilson, I. A., Wardemann, H., Julien, J. P. & Levashina, E. A., Nov 2 2020, In : The Journal of experimental medicine. 217, 11

Research output: Contribution to journalArticle

Open Access

A highly conserved cryptic epitope in the receptor binding domains of SARS-CoV-2 and SARS-CoV

Yuan, M., Wu, N. C., Zhu, X., Lee, C. C. D., So, R. T. Y., Lv, H., Mok, C. K. P. & Wilson, I. A., May 8 2020, In : Science. 368, 6491, p. 630-633 4 p.

Research output: Contribution to journalArticle

Open Access

An Alternative Binding Mode of IGHV3-53 Antibodies to the SARS-CoV-2 Receptor Binding Domain

Wu, N. C., Yuan, M., Liu, H., Lee, C. C. D., Zhu, X., Bangaru, S., Torres, J. L., Caniels, T. G., Brouwer, P. J. M., van Gils, M. J., Sanders, R. W., Ward, A. B. & Wilson, I. A., Sep 28 2020, (Accepted/In press) In : Cell Reports. 108274.

Research output: Contribution to journalArticle

Open Access

A natural mutation between SARS-CoV-2 and SARS-CoV determines neutralization by a cross-reactive antibody

Wu, N. C., Yuan, M., Bangaru, S., Huang, D., Zhu, X., Lee, C-C. D., Turner, H. L., Peng, L., Yang, L., Nemazee, D., Ward, A. B. & Wilson, I. A., Sep 21 2020, (In preparation) Cold Spring Harbor Laboratory Press, 37 p. (bioRxiv).

Research output: Working paper

An influenza A hemagglutinin small-molecule fusion inhibitor identified by a new high-throughput fluorescence polarization screen

Yao, Y., Kadam, R. U., Lee, C. C. D., Woehl, J. L., Wu, N. C., Zhu, X., Kitamura, S., Wilson, I. A. & Wolan, D. W., Aug 4 2020, In : Proceedings of the National Academy of Sciences of the United States of America. 117, 31, p. 18431-18438 8 p.

Research output: Contribution to journalArticle

Open Access

Benchmarking of computational error-correction methods for next-generation sequencing data

Mitchell, K., Brito, J. J., Mandric, I., Wu, Q., Knyazev, S., Chang, S., Martin, L. S., Karlsberg, A., Gerasimov, E., Littman, R., Hill, B. L., Wu, N. C., Yang, H. T., Hsieh, K., Chen, L., Littman, E., Shabani, T., Enik, G., Yao, D., Sun, R. & 6 others, Schroeder, J., Eskin, E., Zelikovsky, A., Skums, P., Pop, M. & Mangul, S., Mar 17 2020, In : Genome biology. 21, 1, 71.

Research output: Contribution to journalArticle

Open Access

Convergent Evolution in Breadth of Two VH6-1-Encoded Influenza Antibody Clonotypes from a Single Donor

Wu, N. C., Andrews, S. F., Raab, J. E., O'Connell, S., Schramm, C. A., Ding, X., Chambers, M. J., Leung, K., Wang, L., Zhang, Y., Mascola, J. R., Douek, D. C., Ledgerwood, J. E., McDermott, A. B. & Wilson, I. A., 2020, (Accepted/In press) In : Cell Host and Microbe. 28, 3, p. 434-444.e4

Research output: Contribution to journalArticle

Open Access

COVID-19 vaccines: Knowing the unknown

Lv, H., Wu, N. C. & Mok, C. K. P., Jul 1 2020, In : European Journal of Immunology. 50, 7, p. 939-943 5 p.

Research output: Contribution to journalComment/debate

Open Access

Cross-reactive Antibody Response between SARS-CoV-2 and SARS-CoV Infections

Lv, H., Wu, N. C., Tsang, O. T. Y., Yuan, M., Perera, R. A. P. M., Leung, W. S., So, R. T. Y., Chan, J. M. C., Yip, G. K., Chik, T. S. H., Wang, Y., Choi, C. Y. C., Lin, Y., Ng, W. W., Zhao, J., Poon, L. L. M., Peiris, J. S. M., Wilson, I. A. & Mok, C. K. P., Jun 2 2020, In : Cell Reports. 31, 9, 107725.

Research output: Contribution to journalArticle

Open Access

Different genetic barriers for resistance to HA stem antibodies in influenza H3 and H1 viruses

Wu, N. C., Thompson, A. J., Lee, J. M., Su, W., Arlian, B. M., Xie, J., Lerner, R. A., Yen, H. L., Bloom, J. D. & Wilson, I. A., Jun 19 2020, In : Science. 368, 6497, p. 1335-1340 6 p.

Research output: Contribution to journalArticle

Evidence of antigenic imprinting in sequential Sarbecovirus immunization

Lv, H., So, R. T. Y., Yuan, M., Liu, H., Lee, C-C. D., Yip, G. K., Ng, W. W., Wilson, I. A., Peiris, J. S. M., Wu, N. C. & Mok, C. K. P., Oct 15 2020, (In preparation) Cold Spring Harbor Laboratory Press, 15 p. (bioRxiv).

Research output: Working paper

Immunity against cancer cells may promote their proliferation and metastasis

Lin, C. W., Xie, J., Zhang, D., Han, K. H., Grande, G., Wu, N. C., Yang, Z., Yea, K. & Lerner, R. A., Jan 7 2020, In : Proceedings of the National Academy of Sciences of the United States of America. 117, 1, p. 426-431 6 p.

Research output: Contribution to journalArticle

Open Access

Influenza hemagglutinin structures and antibody recognition

Wu, N. C. & Wilson, I. A., Aug 2020, In : Cold Spring Harbor perspectives in medicine. 10, 8, p. 1-20 20 p., a038778.

Research output: Contribution to journalArticle

Open Access

Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model

Rogers, T. F., Zhao, F., Huang, D., Beutler, N., Burns, A., He, W. T., Limbo, O., Smith, C., Song, G., Woehl, J., Yang, L., Abbott, R. K., Callaghan, S., Garcia, E., Hurtado, J., Parren, M., Peng, L., Ramirez, S., Ricketts, J., Ricciardi, M. J. & 14 others, Rawlings, S. A., Wu, N. C., Yuan, M., Smith, D. M., Nemazee, D., Teijaro, J. R., Voss, J. E., Wilson, I. A., Andrabi, R., Briney, B., Landais, E., Sok, D., Jardine, J. G. & Burton, D. R., Aug 21 2020, In : Science (New York, N.Y.). 369, 6506, p. 956-963 8 p.

Research output: Contribution to journalArticle

Open Access

Major antigenic site B of human influenza H3N2 viruses has an evolving local fitness landscape

Wu, N. C., Otwinowski, J., Thompson, A. J., Nycholat, C. M., Nourmohammad, A. & Wilson, I. A., Mar 6 2020, In : Nature communications. 11, 1, 1233.

Research output: Contribution to journalArticle

Open Access

Recognition of the SARS-CoV-2 receptor binding domain by neutralizing antibodies

Yuan, M., Liu, H., Wu, N. C. & Wilson, I. A., Oct 10 2020, (Accepted/In press) In : Biochemical and Biophysical Research Communications.

Research output: Contribution to journalArticle

Open Access

Serological assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), March 2020

Perera, R. A. P. M., Mok, C. K. P., Tsang, O. T. Y., Lv, H., Ko, R. L. W., Wu, N. C., Yuan, M., Leung, W. S., Chan, J. M. C., Chik, T. S. H., Choi, C. Y. C., Leung, K., Chan, K. H., Chan, K. C. K., Li, K. C., Wu, J. T., Wilson, I. A., Monto, A. S., Poon, L. L. M. & Peiris, M., 2020, In : Eurosurveillance. 25, 16, p. 14-22 2000421.

Research output: Contribution to journalArticle

Open Access

Structural basis of a shared antibody response to SARS-CoV-2

Yuan, M., Liu, H., Wu, N. C., Lee, C. C. D., Zhu, X., Zhao, F., Huang, D., Yu, W., Hua, Y., Tien, H., Rogers, T. F., Landais, E., Sok, D., Jardine, J. G., Burton, D. R. & Wilson, I. A., Aug 28 2020, In : Science (New York, N.Y.). 369, 6507, p. 1119-1123 5 p.

Research output: Contribution to journalArticle

Open Access

Structural Biology of Influenza Hemagglutinin: An Amaranthine Adventure

Wu, N. C. & Wilson, I. A., Sep 22 2020, In : Viruses. 12, 9, 1053.

Research output: Contribution to journalReview article

Open Access
2019

Application of deep mutational scanning in hepatitis C virus

Wu, N. C. & Qi, H., 2019, Methods in Molecular Biology. Humana Press Inc., p. 183-190 8 p. (Methods in Molecular Biology; vol. 1911).

Research output: Chapter in Book/Report/Conference proceedingChapter

Bacterial glycosyltransferase-mediated cell-surface chemoenzymatic glycan modification

Hong, S., Shi, Y., Wu, N. C., Grande, G., Douthit, L., Wang, H., Zhou, W., Sharpless, K. B., Wilson, I. A., Xie, J. & Wu, P., Dec 1 2019, In : Nature communications. 10, 1, 1799.

Research output: Contribution to journalArticle

Open Access

Conformational Plasticity in the HIV-1 Fusion Peptide Facilitates Recognition by Broadly Neutralizing Antibodies

Yuan, M., Cottrell, C. A., Ozorowski, G., van Gils, M. J., Kumar, S., Wu, N. C., Sarkar, A., Torres, J. L., de Val, N., Copps, J., Moore, J. P., Sanders, R. W., Ward, A. B. & Wilson, I. A., Jun 12 2019, In : Cell Host and Microbe. 25, 6, p. 873-883.e5

Research output: Contribution to journalArticle

Open Access

Identification of Antibodies Targeting the H3N2 Hemagglutinin Receptor Binding Site following Vaccination of Humans

Zost, S. J., Lee, J., Gumina, M. E., Parkhouse, K., Henry, C., Wu, N. C., Lee, C. C. D., Wilson, I. A., Wilson, P. C., Bloom, J. D. & Hensley, S. E., Dec 24 2019, In : Cell Reports. 29, 13, p. 4460-4470.e8

Research output: Contribution to journalArticle

Open Access

Immunodominance and Antigenic Variation of Influenza Virus Hemagglutinin: Implications for Design of Universal Vaccine Immunogens

Zost, S. J., Wu, N. C., Hensley, S. E. & Wilson, I. A., Apr 8 2019, In : Journal of Infectious Diseases. 219, p. S38-S45

Research output: Contribution to journalArticle

MiCoP: Microbial community profiling method for detecting viral and fungal organisms in metagenomic samples

Lapierre, N., Mangul, S., Alser, M., Mandric, I., Wu, N. C., Koslicki, D. & Eskin, E., Jun 6 2019, In : BMC genomics. 20, 423.

Research output: Contribution to journalArticle

Open Access

Multistate design of influenza antibodies improves affinity and breadth against seasonal viruses

Sevy, A. M., Wu, N. C., Gilchuk, I. M., Parrish, E. H., Burger, S., Yousif, D., Nagel, M. B. M., Schey, K. L., Wilson, I. A., Crowe, J. E. & Meiler, J., Jan 29 2019, In : Proceedings of the National Academy of Sciences of the United States of America. 116, 5, p. 1597-1602 6 p.

Research output: Contribution to journalArticle

Open Access

Preventing an Antigenically Disruptive Mutation in Egg-Based H3N2 Seasonal Influenza Vaccines by Mutational Incompatibility

Wu, N. C., Lv, H., Thompson, A. J., Wu, D. C., Ng, W. W. S., Kadam, R. U., Lin, C. W., Nycholat, C. M., McBride, R., Liang, W., Paulson, J. C., Mok, C. K. P. & Wilson, I. A., Jun 12 2019, In : Cell Host and Microbe. 25, 6, p. 836-844.e5

Research output: Contribution to journalArticle

Open Access

The in vivo ISGylome links ISG15 to metabolic pathways and autophagy upon Listeria monocytogenes infection

Zhang, Y., Thery, F., Wu, N. C., Luhmann, E. K., Dussurget, O., Foecke, M., Bredow, C., Jiménez-Fernández, D., Leandro, K., Beling, A., Knobeloch, K. P., Impens, F., Cossart, P. & Radoshevich, L., Dec 1 2019, In : Nature communications. 10, 1, 5383.

Research output: Contribution to journalArticle

Open Access
2018

A complex epistatic network limits the mutational reversibility in the influenza hemagglutinin receptor-binding site

Wu, N. C., Thompson, A. J., Xie, J., Lin, C. W., Nycholat, C. M., Zhu, X., Lerner, R. A., Paulson, J. C. & Wilson, I. A., Dec 1 2018, In : Nature communications. 9, 1, 1264.

Research output: Contribution to journalArticle

Open Access

Clinical correlations of transcriptional profile in patients infected with avian influenza H7N9 virus

Guan, W., Yang, Z., Wu, N. C., Lee, H. H. Y., Li, Y., Jiang, W., Shen, L., Wu, D. C., Chen, R., Zhong, N., Wilson, I. A., Peiris, M. & Mok, C. K. P., Sep 8 2018, In : Journal of Infectious Diseases. 218, 8, p. 1238-1248 11 p.

Research output: Contribution to journalArticle

Open Access

Deception through Mimicry: A Cellular Antiviral Strategy

Wu, N. C. & Ward, A. B., Dec 13 2018, In : Cell. 175, 7, p. 1728-1729 2 p.

Research output: Contribution to journalShort survey

Open Access

Deep mutational scanning of hemagglutinin helps predict evolutionary fates of human H3N2 influenza variants

Lee, J. M., Huddleston, J., Doud, M. B., Hooper, K. A., Wu, N. C., Bedford, T. & Bloom, J. D., Aug 28 2018, In : Proceedings of the National Academy of Sciences of the United States of America. 115, 35, p. E8276-E8285

Research output: Contribution to journalArticle

Open Access

Fully human agonist antibodies to TrkB using autocrine cell-based selection from a combinatorial antibody library

Merkouris, S., Barde, Y. A., Binley, K. E., Allen, N. D., Stepanov, A. V., Wu, N. C., Grande, G., Lin, C. W., Li, M., Nan, X., Chacon-Fernandez, P., DiStefano, P. S., Lindsay, R. M., Lerner, R. A. & Xie, J., Jul 24 2018, In : Proceedings of the National Academy of Sciences of the United States of America. 115, 30, p. E7023-E7032

Research output: Contribution to journalArticle

Open Access

Genome-wide identification of interferon-sensitive mutations enables influenza vaccine design

Du, Y., Xin, L., Shi, Y., Zhang, T. H., Wu, N. C., Dai, L., Gong, D., Brar, G., Shu, S., Luo, J., Reiley, W., Tseng, Y. W., Bai, H., Wu, T. T., Wang, J., Shu, Y. & Sun, R., Jan 19 2018, In : Science. 359, 6373, p. 290-296 7 p.

Research output: Contribution to journalArticle

Open Access

Recurring and Adaptable Binding Motifs in Broadly Neutralizing Antibodies to Influenza Virus Are Encoded on the D3-9 Segment of the Ig Gene

Wu, N. C., Yamayoshi, S., Ito, M., Uraki, R., Kawaoka, Y. & Wilson, I. A., Oct 10 2018, In : Cell Host and Microbe. 24, 4, p. 569-578.e4

Research output: Contribution to journalArticle

Open Access

Structural insights into the design of novel anti-influenza therapies

Wu, N. C. & Wilson, I. A., Feb 1 2018, In : Nature Structural and Molecular Biology. 25, 2, p. 115-121 7 p.

Research output: Contribution to journalReview article

Structure-Activity Relationships in Metal-Binding Pharmacophores for Influenza Endonuclease

Credille, C. V., Dick, B. L., Morrison, C. N., Stokes, R. W., Adamek, R. N., Wu, N. C., Wilson, I. A. & Cohen, S. M., Nov 21 2018, In : Journal of Medicinal Chemistry. 61, 22, p. 10206-10217 12 p.

Research output: Contribution to journalArticle

2017

A Broadly Neutralizing Antibody Targets the Dynamic HIV Envelope Trimer Apex via a Long, Rigidified, and Anionic β-Hairpin Structure

Lee, J. H., Andrabi, R., Su, C. Y., Yasmeen, A., Julien, J. P., Kong, L., Wu, N. C., McBride, R., Sok, D., Pauthner, M., Cottrell, C. A., Nieusma, T., Blattner, C., Paulson, J. C., Klasse, P. J., Wilson, I. A., Burton, D. R. & Ward, A. B., Apr 18 2017, In : Immunity. 46, 4, p. 690-702 13 p.

Research output: Contribution to journalArticle

Open Access

Antibody 27F3 Broadly Targets Influenza A Group 1 and 2 Hemagglutinins through a Further Variation in VH1-69 Antibody Orientation on the HA Stem

Lang, S., Xie, J., Zhu, X., Wu, N. C., Lerner, R. A. & Wilson, I. A., Sep 19 2017, In : Cell Reports. 20, 12, p. 2935-2943 9 p.

Research output: Contribution to journalArticle

Open Access

A Perspective on the Structural and Functional Constraints for Immune Evasion: Insights from Influenza Virus

Wu, N. C. & Wilson, I. A., Aug 18 2017, In : Journal of Molecular Biology. 429, 17, p. 2694-2709 16 p.

Research output: Contribution to journalReview article

A structural explanation for the low effectiveness of the seasonal influenza H3N2 vaccine

Wu, N. C., Zost, S. J., Thompson, A. J., Oyen, D., Nycholat, C. M., McBride, R., Paulson, J. C., Hensley, S. E. & Wilson, I. A., Oct 2017, In : PLoS pathogens. 13, 10, e1006682.

Research output: Contribution to journalArticle

Open Access

Diversity of Functionally Permissive Sequences in the Receptor-Binding Site of Influenza Hemagglutinin

Wu, N. C., Xie, J., Zheng, T., Nycholat, C. M., Grande, G., Paulson, J. C., Lerner, R. A. & Wilson, I. A., Jun 14 2017, In : Cell Host and Microbe. 21, 6, p. 742-753.e8

Research output: Contribution to journalArticle

Open Access

Immunochemical engineering of cell surfaces to generate virus resistance

Xie, J., Sok, D., Wu, N. C., Zheng, T., Zhang, W., Burton, D. R. & Lerner, R. A., May 2 2017, In : Proceedings of the National Academy of Sciences of the United States of America. 114, 18, p. 4655-4660 6 p.

Research output: Contribution to journalArticle

Open Access

In vitro evolution of an influenza broadly neutralizing antibody is modulated by hemagglutinin receptor specificity

Wu, N. C., Grande, G., Turner, H. L., Ward, A. B., Xie, J., Lerner, R. A. & Wilson, I. A., May 15 2017, In : Nature communications. 8, 15371.

Research output: Contribution to journalArticle

Open Access

Long Single-Molecule Reads Can Resolve the Complexity of the Influenza Virus Composed of Rare, Closely Related Mutant Variants

Artyomenko, A., Wu, N. C., Mangul, S., Eskin, E., Sun, R. & Zelikovsky, A., Jun 2017, In : Journal of Computational Biology. 24, 6, p. 558-570 13 p.

Research output: Contribution to journalArticle

Quantifying perinatal transmission of Hepatitis B viral quasispecies by tag linkage deep sequencing

Du, Y., Chi, X., Wang, C., Jiang, J., Kong, F., Yan, H., Wang, X., Li, J., Wu, N. C., Dai, L., Zhang, T. H., Shu, S., Zhou, J., Yoshizawa, J. M., Li, X., Bhattacharya, D., Wu, T. T., Niu, J. & Sun, R., Dec 1 2017, In : Scientific reports. 7, 1, 10168.

Research output: Contribution to journalArticle

Open Access

Structure-based optimization and synthesis of antiviral drug Arbidol analogues with significantly improved affinity to influenza hemagglutinin

Wright, Z. V. F., Wu, N. C., Kadam, R. U., Wilson, I. A. & Wolan, D. W., 2017, In : Bioorganic and Medicinal Chemistry Letters. 27, 16, p. 3744-3748 5 p.

Research output: Contribution to journalArticle

Systematic identification of anti-interferon function on hepatitis C virus genome reveals p7 as an immune evasion protein

Qi, H., Chu, V., Wu, N. C., Chen, Z., Truong, S., Brar, G., Su, S. Y., Du, Y., Arumugaswami, V., Olson, C. A., Chen, S. H., Lin, C. Y., Wu, T. T. & Sun, R., Feb 21 2017, In : Proceedings of the National Academy of Sciences of the United States of America. 114, 8, p. 2018-2023 6 p.

Research output: Contribution to journalArticle

Open Access
2016

A benchmark study on error-correction by read-pairing and tag-clustering in amplicon-based deep sequencing

Zhang, T. H., Wu, N. C. & Sun, R., Feb 12 2016, In : BMC genomics. 17, 1, 108.

Research output: Contribution to journalArticle

Open Access

Adaptation in protein fitness landscapes is facilitated by indirect paths

Wu, N. C., Dai, L., Olson, C. A., Lloyd-Smith, J. O. & Sun, R., Jul 8 2016, In : eLife. 5, JULY, e16965.

Research output: Contribution to journalArticle

Open Access