Lester Perez Rodriguez

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Research Output

2020
Open Access
2019

Investigation of chronic and persistent classical swine fever infections under field conditions and their impact on vaccine efficacy

Coronado, L., Bohórquez, J. A., Muñoz-González, S., Perez, L. J., Rosell, R., Fonseca, O., Delgado, L., Perera, C. L., Frías, M. T. & Ganges, L., Jul 15 2019, In : BMC Veterinary Research. 15, 1, 247.

Research output: Contribution to journalArticle

Open Access

Positive selection pressure on E2 protein of classical swine fever virus drives variations in virulence, pathogenesis and antigenicity: Implication for epidemiological surveillance in endemic areas

Coronado, L., Rios, L., Frías, M. T., Amarán, L., Naranjo, P., Percedo, M. I., Perera, C. L., Prieto, F., Fonseca-Rodriguez, O. & Perez, L. J., Nov 1 2019, In : Transboundary and Emerging Diseases. 66, 6, p. 2362-2382 21 p.

Research output: Contribution to journalArticle

Impact of RNA degradation on viral diagnosis: An understated but essential step for the successful establishment of a diagnosis network

Relova, D., Rios, L., Acevedo, A. M., Coronado, L., Perera, C. L. & Pérez, L. J., Mar 1 2018, In : Veterinary Sciences. 5, 1, 19.

Research output: Contribution to journalArticle

Open Access

Multi-target strategy for pan/foot-and-mouth disease virus (FMDV) detection: A combination of sequences analysis, in silico predictions and laboratory diagnostic evaluation

Rios, L., Perera, C. L., Coronado, L., Relova, D., álvarez, A. M., Ganges, L., de Arce, H. D., Núñez, J. I. & Pérez, L. J., Jul 12 2018, In : Frontiers in Veterinary Science. 5, JUL, 160.

Research output: Contribution to journalArticle

Open Access

Revisiting the genetic diversity of classical swine fever virus: A proposal for new genotyping and subgenotyping schemes of classification

Rios, L., Núñez, J. I., Díaz de Arce, H., Ganges, L. & Pérez, L. J., Aug 2018, In : Transboundary and Emerging Diseases. 65, 4, p. 963-971 9 p.

Research output: Contribution to journalArticle

2017

Deciphering the emergence, genetic diversity and evolution of classical swine fever virus

Rios, L., Coronado, L., Naranjo-Feliciano, D., Martínez-Pérez, O., Perera, C. L., Hernandez-Alvarez, L., Díaz De Arce, H., Núñez, J. I., Ganges, L. & Pérez, L. J., Dec 1 2017, In : Scientific reports. 7, 1, 17887.

Research output: Contribution to journalArticle

Open Access

Differential expression of porcine microRNAs in African swine fever virus infected pigs: A proof-of-concept study

Núñez-Hernández, F., Pérez, L. J., Muñoz, M., Vera, G., Accensi, F., Sánchez, A., Rodríguez, F. & Núñez, J. I., Oct 17 2017, In : Virology Journal. 14, 1, 198.

Research output: Contribution to journalArticle

Open Access

Lysophosphatidic acid receptor mRNA levels in heart and white adipose tissue are associated with obesity in mice and humans

Brown, A., Hossain, I., Perez, L. J., Nzirorera, C., Tozer, K., D’Souza, K., Trivedi, P. C., Aguiar, C., Yip, A. M., Shea, J., Brunt, K. R., Legare, J. F., Hassan, A., Pulinilkunnil, T. & Kienesberger, P. C., Dec 2017, In : PloS one. 12, 12, e0189402.

Research output: Contribution to journalArticle

Open Access

Novel poly-uridine insertion in the 3′UTR and E2 amino acid substitutions in a low virulent classical swine fever virus

Coronado, L., Liniger, M., Muñoz-González, S., Postel, A., Pérez, L. J., Pérez-Simó, M., Perera, C. L., Frías- Lepoureau, M. T., Rosell, R., Grundhoff, A., Indenbirken, D., Alawi, M., Fischer, N., Becher, P., Ruggli, N. & Ganges, L., Mar 1 2017, In : Veterinary Microbiology. 201, p. 103-112 10 p.

Research output: Contribution to journalArticle

Open Access

Stability and integrity of classical swine fever virus RNA stored at room temperature

Relova, D., Pérez, L. J., Ríos, L., Coronado, L., Hinojosa, Y., Acevedo, A. M., Frías, M. T. & Perera, C. L., Jan 1 2017, In : Spanish Journal of Agricultural Research. 15, 3, e05SC03.

Research output: Contribution to journalArticle

Open Access

Tracking the Origin and Deciphering the Phylogenetic Relationship of Porcine Epidemic Diarrhea Virus in Ecuador

Barrera, M., Garrido-Haro, A., Vaca, M. S., Granda, D., Acosta-Batallas, A. & Pérez, L. J., Jan 1 2017, In : BioMed Research International. 2017, 2978718.

Research output: Contribution to journalArticle

Open Access

Validation of optimal reference genes for quantitative real time PCR in muscle and adipose tissue for obesity and diabetes research

Perez, L. J., Rios, L., Trivedi, P., D'Souza, K., Cowie, A., Nzirorera, C., Webster, D., Brunt, K., Legare, J. F., Hassan, A., Kienesberger, P. C. & Pulinilkunnil, T., Dec 1 2017, In : Scientific reports. 7, 1, 3612.

Research output: Contribution to journalArticle

Open Access
2016

Classical swine fever virus vs. Classical swine fever virus: The superinfection exclusion phenomenon in experimentally infected wild boar

Muñoz-González, S., Pérez-Simó, M., Colom-Cadena, A., Cabezón, O., Bohórquez, J. A., Rosell, R., Pérez, L. J., Marco, I., Lavín, S., Domingo, M. & Ganges, L., Feb 2016, In : PloS one. 11, 2, e0149469.

Research output: Contribution to journalArticle

Open Access

Glucolipotoxicity diminishes cardiomyocyte TFEB and inhibits lysosomal autophagy during obesity and diabetes

Trivedi, P. C., Bartlett, J. J., Perez, L. J., Brunt, K. R., Legare, J. F., Hassan, A., Kienesberger, P. C. & Pulinilkunnil, T., Dec 1 2016, In : Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. 1861, 12, p. 1893-1910 18 p.

Research output: Contribution to journalArticle

2015

Development of a new LAMP assay for the detection of CSFV strains from Cuba: a proof-of-concept study

Postel, A., Pérez, L. J., Perera, C. L., Schmeiser, S., Meyer, D., Meindl-Boehmer, A., Rios, L., Austermann-Busch, S., Frias-Lepoureau, M. T. & Becher, P., Jun 1 2015, In : Archives of Virology. 160, 6, p. 1435-1448 14 p.

Research output: Contribution to journalArticle

Evaluation of a phylogenetic marker based on genomic segment B of infectious bursal disease virus: Facilitating a feasible incorporation of this segment to the molecular epidemiology studies for this viral agent

Alfonso-Morales, A., Rios, L., Martínez-Pérez, O., Dolz, R., Valle, R., Perera, C. L., Bertran, K., Frías, M. T., Ganges, L., De Arce, H. D., Majó, N., Núñez, J. I. & Pérez, L. J., May 6 2015, In : PloS one. 10, 5, e0125853.

Research output: Contribution to journalArticle

Open Access

Evaluation of the capability of the PCV2 genome to encode miRNAs: Lack of viral miRNA expression in an experimental infection

Núñez-Hernández, F., Pérez, L. J., Vera, G., Córdoba, S., Segalés, J., Sánchez, A. & Núñez, J. I., May 1 2015, In : Veterinary research. 46, 1, 48.

Research output: Contribution to journalArticle

Open Access

Identification of microRNAs in PCV2 subclinically infected pigs by high throughput sequencing

Núñez-Hernández, F., Pérez, L. J., Muñoz, M., Vera, G., Tomás, A., Egea, R., Córdoba, S., Segalés, J., Sánchez, A. & Núñez, J. I., Jan 1 2015, In : Veterinary research. 46, 1, 18.

Research output: Contribution to journalArticle

Open Access

Molecular epidemiology study of swine influenza virus revealing a reassorted virus H1N1 in swine farms in Cuba

Pérez, L. J., Perera, C. L., Coronado, L., Rios, L., Vega, A., Frías, M. T., Ganges, L., Núñez, J. I. & Díaz de Arce, H., May 1 2015, In : Preventive Veterinary Medicine. 119, 3-4, p. 172-178 7 p.

Research output: Contribution to journalArticle

Postnatal persistent infection with classical swine fever virus and its immunological implications

Muñoz-González, S., Ruggli, N., Rosell, R., Pérez, L. J., Frías-Leuporeau, M. T., Fraile, L., Montoya, M., Cordoba, L., Domingo, M., Ehrensperger, F., Summerfield, A. & Ganges, L., May 4 2015, In : PloS one. 10, 5, e0125692.

Research output: Contribution to journalArticle

Open Access
2014

Genetic characterization of influenza A viruses circulating in pigs and isolated in north-east Spain during the period 2006-2007

Baratelli, M., Córdoba, L., Pérez, L. J., Maldonado, J., Fraile, L., Núñez, J. I. & Montoya, M., Apr 2014, In : Research in Veterinary Science. 96, 2, p. 380-388 9 p.

Research output: Contribution to journalArticle

The impact of CSFV on the immune response to control infection

Tarradas, J., de la Torre, M. E., Rosell, R., Perez, L. J., Pujols, J., Muñoz, M., Muñoz, I., Muñoz, S., Abad, X., Domingo, M., Fraile, L. & Ganges, L., Jun 24 2014, In : Virus Research. 185, p. 82-91 10 p.

Research output: Contribution to journalArticle

The role of viral and host microRNAs in the Aujeszky's disease virus during the infection process

Timoneda, O., Núñez-Hernández, F., Balcells, I., Muñoz, M., Castelló, A., Vera, G., Pérez, L. J., Egea, R., Mir, G., Córdoba, S., Rosell, R., Segalés, J., Tomàs, A., Sánchez, A. & Núñez, J. I., Jan 24 2014, In : PloS one. 9, 1, e86965.

Research output: Contribution to journalArticle

Open Access
2013

A duplex SYBR Green I-based real-time RT-PCR assay for the simultaneous detection and differentiation of Massachusetts and non-Massachusetts serotypes of infectious bronchitis virus

Acevedo, A. M., Perera, C. L., Vega, A., Ríos, L., Coronado, L., Relova, D., Frías, M. T., Ganges, L., Núñez, J. I. & Pérez, L. J., Oct 1 2013, In : Molecular and Cellular Probes. 27, 5-6, p. 184-192 9 p.

Research output: Contribution to journalArticle

Classical swine fever virus isolates from Cuba form a new subgenotype 1.4

Postel, A., Schmeiser, S., Perera, C. L., Rodríguez, L. J. P., Frias-Lepoureau, M. T. & Becher, P., Jan 25 2013, In : Veterinary Microbiology. 161, 3-4, p. 334-338 5 p.

Research output: Contribution to journalArticle

Isolation and complete genomic characterization of pandemic H1N1/2009 influenza viruses from Cuban swine herds

Pérez, L. J., Perera, C. L., Vega, A., Frías, M. T., Rouseaux, D., Ganges, L., Nuñez, J. I. & Díaz de Arce, H., Jun 1 2013, In : Research in Veterinary Science. 94, 3, p. 781-788 8 p.

Research output: Contribution to journalArticle

Phylogenetic analysis of human T cell lymphotropic virus type 1 isolated from cuban individuals

Machado, L. Y., Navea, L. M., Díaz, H. M., Blanco, M., Dubed, M., Romay, D. M., Silva, E. & Pérez, L. J., Aug 1 2013, In : AIDS Research and Human Retroviruses. 29, 8, p. 1168-1172 5 p.

Research output: Contribution to journalArticle

Phylogenetic and molecular characterization of coronavirus affecting species of bovine and birds in Cuba

Acevedo, A. M., Martínez, N., Brandão, P., Perera, C. L., Frías, M. T., Barrera, M. & Pérez, L. J., Jul 1 2013, In : Biotecnologia Aplicada. 30, 3, p. 228-231 4 p.

Research output: Contribution to journalArticle

Spatiotemporal Phylogenetic Analysis and Molecular Characterisation of Infectious Bursal Disease Viruses Based on the VP2 Hyper-Variable Region

Alfonso-Morales, A., Martínez-Pérez, O., Dolz, R., Valle, R., Perera, C. L., Bertran, K., Frías, M. T., Majó, N., Ganges, L. & Pérez, L. J., Jun 21 2013, In : PloS one. 8, 6, e65999.

Research output: Contribution to journalArticle

Open Access
2012

A multiple SYBR Green I-based real-time PCR system for the simultaneous detection of porcine circovirus type 2, porcine parvovirus, pseudorabies virus and Torque teno sus virus 1 and 2 in pigs

Pérez, L. J., Perera, C. L., Frías, M. T., Núñez, J. I., Ganges, L. & Arce, H. D. D., Jan 1 2012, In : Journal of Virological Methods. 179, 1, p. 233-241 9 p.

Research output: Contribution to journalArticle

An SYBR Green-based real-time RT-PCR assay for the detection of H5 hemagglutinin subtype avian influenza virus

Pérez, L. J., Díaz de Arce, H., Cilloni, F., Salviato, A., Marciano, S., Perera, C. L., Salomoni, A., Beato, M. S., Romero, A., Capua, I. & Cattoli, G., Jun 1 2012, In : Molecular and Cellular Probes. 26, 3, p. 137-145 9 p.

Research output: Contribution to journalArticle

A T-cell epitope on NS3 non-structural protein enhances the B and T cell responses elicited by dendrimeric constructions against CSFV in domestic pigs

Tarradas, J., Monsó, M., Fraile, L., de la Torre, B. G., Muñoz, M., Rosell, R., Riquelme, C., Pérez, L. J., Nofrarías, M., Domingo, M., Sobrino, F., Andreu, D. & Ganges, L., Nov 15 2012, In : Veterinary Immunology and Immunopathology. 150, 1-2, p. 36-46 11 p.

Research output: Contribution to journalArticle

First evidence of the emergence of novel putative infectious bronchitis virus genotypes in Cuba

Acevedo, A. M., Díaz de Arce, H., Brandão, P. E., Colas, M., Oliveira, S. & Pérez, L. J., Oct 1 2012, In : Research in Veterinary Science. 93, 2, p. 1046-1049 4 p.

Research output: Contribution to journalArticle

Molecular and phylogenetic analysis of bovine coronavirus based on the spike glycoprotein gene

Martínez, N., Brandão, P. E., de Souza, S. P., Barrera, M., Santana, N., de Arce, H. D. & Pérez, L. J., Dec 1 2012, In : Infection, Genetics and Evolution. 12, 8, p. 1870-1878 9 p.

Research output: Contribution to journalArticle

Positive selection pressure on the B/C domains of the E2-gene of classical swine fever virus in endemic areas under C-strain vaccination

Pérez, L. J., Díaz de Arce, H., Perera, C. L., Rosell, R., Frías, M. T., Percedo, M. I., Tarradas, J., Dominguez, P., Núñez, J. I. & Ganges, L., Oct 1 2012, In : Infection, Genetics and Evolution. 12, 7, p. 1405-1412 8 p.

Research output: Contribution to journalArticle

2011

Development and validation of a novel SYBR Green real-time RT-PCR assay for the detection of classical swine fever virus evaluated on different real-time PCR platforms

Pérez, L. J., Díaz de Arce, H., Tarradas, J., Rosell, R., Perera, C. L., Muñoz, M., Frías, M. T., Nuñez, J. I. & Ganges, L., Jun 1 2011, In : Journal of Virological Methods. 174, 1-2, p. 53-59 7 p.

Research output: Contribution to journalArticle

Molecular detection of Torque teno sus virus in lymphoid tissues in concomitant infections with other porcine viral pathogens

Pérez, L. J., Arce, H. D. D., Frias, M. T., Perera, C. L., Ganges, L. & Núñez, J. I., Dec 1 2011, In : Research in Veterinary Science. 91, 3, p. e154-e157

Research output: Contribution to journalArticle

Phylogenetic networks to study the origin and evolution of porcine circovirus type 2 (PCV2) in Cuba

Pérez, L. J., De Arce, H. D., Cortey, M., Domínguez, P., Percedo, M. I., Perera, C. L., Tarradas, J., Frías, M. T., Segalés, J., Ganges, L. & Núñez, J. I., Aug 5 2011, In : Veterinary Microbiology. 151, 3-4, p. 245-254 10 p.

Research output: Contribution to journalArticle

2010

First report of porcine circovirus type 2 infections in Cuba

Pérez, L. J., Díaz de Arce, H., Percedo, M. I., Domínguez, P. & Frías, M. T., Jun 1 2010, In : Research in Veterinary Science. 88, 3, p. 528-530 3 p.

Research output: Contribution to journalArticle

Genetic characterization and phylogenetic analysis of porcine circovirus type 2 strains present in Cuban swine herds

Pérez, L. J., De Arce, H. D. & Frías, M. T., Oct 1 2010, In : Research in Veterinary Science. 89, 2, p. 301-305 5 p.

Research output: Contribution to journalArticle

2009

A multiplex RT-PCR assay for the rapid and differential diagnosis of classical swine fever and other pestivirus infections

Arce, H. D. D., Pérez, L. J., Frías, M. T., Rosell, R., Tarradas, J., Núñez, J. I. & Ganges, L., Nov 18 2009, In : Veterinary Microbiology. 139, 3-4, p. 245-252 8 p.

Research output: Contribution to journalArticle

A RT-PCR assay for the detection of encephalomyocarditis virus infections in pigs

Pérez, L. J. & de Arce, H. D., Jan 1 2009, In : Brazilian Journal of Microbiology. 40, 4, p. 988-993 6 p.

Research output: Contribution to journalArticle

Open Access

Desenvolvimento de um ensaio de Reação de Polimerase em Cadeia para detecção do vírus da pseudo-raiva em amostras clínicas

Translated title of the contribution: Development of a polymerase chain reaction assay for the detection of pseudorabies virus in clinical samplesPérez, L. J. & de Arce, H. D., Jan 1 2009, In : Brazilian Journal of Microbiology. 40, 3, p. 433-438 6 p.

Research output: Contribution to journalArticle

Open Access