John Alan Gerlt

1973 …2019
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Research Output 1973 2019

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1989

Observation and Sequence Assignment of a Cis Prolyl Peptide Bond in Unliganded Staphylococcal Nuclease

Stanczyk, S. M., Bolton, P. H., Dell'Acqua, M. & Gerlt, J. A., Oct 1 1989, In : Journal of the American Chemical Society. 111, 21, p. 8317-8318 2 p.

Research output: Contribution to journalArticle

Micrococcal Nuclease
Peptides

Selection and Characterization of a Mutant of the Cloned Gene for Mandelate Racemase That Confers Resistance to an Affinity Label by Greatly Enhanced Production of Enzyme

Tsou, A. Y., Ransom, S. C., Gerlt, J. A., Powers, V. M. & Kenyon, G. L., Jan 1 1989, In : Biochemistry. 28, 3, p. 969-975 7 p.

Research output: Contribution to journalArticle

mandelate racemase
Affinity Labels
Genes
Pseudomonas aeruginosa
Pseudomonas putida

Site-specific 13C labeling of DNA to deduce DNA repair mechanisms of uracil-DNA glycosylase and UV endonuclease V

Manoharan, M., Mazumder, A. & Gerlt, J. A., Jan 1 1989, In : Nucleosides and Nucleotides. 8, 5-6, p. 879-883 5 p.

Research output: Contribution to journalArticle

Deoxyribonuclease (Pyrimidine Dimer)
Uracil-DNA Glycosidase
Uracil
Oligodeoxyribonucleotides
Endonucleases

UV Endonuclease V from Bacteriophage T4 Catalyzes DNA Strand Cleavage at Aldehydic Abasic Sites by a Syn β-Elimination Reaction

Mazumder, A., Gerlt, J. A., Rabow, L., Absalon, M. J., Stubbe, J. A. & Bolton, P. H., Sep 1 1989, In : Journal of the American Chemical Society. 111, 20, p. 8029-8030 2 p.

Research output: Contribution to journalArticle

Deoxyribonuclease (Pyrimidine Dimer)
Bacteriophage T4
DNA Cleavage
Bacteriophages
DNA
1988

Cloning, DNA Sequence Analysis, and Expression in Escherichia coli of the Gene for Mandelate Racemase from Pseudomonas putida

Ransom, S. C., Gerlt, J. A., Powers, V. M. & Kenyon, G. L., Jan 1 1988, In : Biochemistry. 27, 2, p. 540-545 6 p.

Research output: Contribution to journalArticle

mandelate racemase
Pseudomonas putida
Cloning
DNA sequences
DNA Sequence Analysis

Direct observation of multiple environments for the Hδ but not the Hε proton of a histidine residue in staphylococcal nuclease

Stanczyk, S. M., Bolton, P. H., Dell'Acqua, M., Pourmotabbed, T. & Gerlt, J. A., Jan 1 1988, In : Journal of the American Chemical Society. 110, 23, p. 7908-7910 3 p.

Research output: Contribution to journalArticle

Micrococcal Nuclease
Histidine
Protons
Observation

Isolation and characterization of a small catalytic domain released from the adenylate cyclase from Escherichia coli by digestion with trypsin

Holland, M. M., Leib, T. K. & Gerlt, J. A., 1988, In : Journal of Biological Chemistry. 263, 29, p. 14661-14668 8 p.

Research output: Contribution to journalArticle

Adenylyl Cyclases
Trypsin
Escherichia coli
Digestion
Catalytic Domain

Mechanism of UV endonuclease V cleavage of abasic sites in DNA determined by 13C labeling

Manoharan, M., Mazumder, A., Ransom, S. C., Gerlt, J. A. & Bolton, P. H., Jan 1 1988, In : Journal of the American Chemical Society. 110, 8, p. 2690-2691 2 p.

Research output: Contribution to journalArticle

Deoxyribonuclease (Pyrimidine Dimer)
Labeling
DNA
UV endonuclease
Endonucleases

Preliminary x-ray data on crystals of mandelate racemase.

Neidhart, D. J., Powers, V. M., Kenyon, G. L., Tsou, A. Y., Ransom, S. C., Gerlt, J. A. & Petsko, G. A., Jan 1 1988, In : The Journal of biological chemistry. 263, 19, p. 9268-9270 3 p.

Research output: Contribution to journalArticle

mandelate racemase
X-Rays
X rays
Crystals
Magnesium Chloride

The Characterization of Abasic Sites in DNA Heteroduplexes by Site Specific Labeling with 13C

Manoharan, M., Ransom, S. C., Mazumder, A., Gerlt, J. A., Wilde, J. A., Withka, J. A. & Bolton, P. H., Jan 1 1988, In : Journal of the American Chemical Society. 110, 5, p. 1620-1622 3 p.

Research output: Contribution to journalArticle

Nucleic Acid Heteroduplexes
Labeling
1987

Coexistence of Conformations in a DNA Heteroduplex Revealed by Site Specific Labeling with 13C-Labeled Nucleotides

Manoharan, M., Gerlt, J. A., Wilde, J. A., Withka, J. M. & Bolton, P. H., Nov 1 1987, In : Journal of the American Chemical Society. 109, 23, p. 7217-7219 3 p.

Research output: Contribution to journalArticle

Nucleic Acid Heteroduplexes
Nucleotides
Labeling
Conformations
DNA
Mutagenesis
Catalysis
Mutant Proteins
Enzymes
Substitution reactions
Mutagenesis
Catalysis
Mutant Proteins
Enzymes
Substitution reactions
Mutagenesis
Catalysis
Mutant Proteins
Enzymes
Substitution reactions

Site-Directed Mutants of Staphylococcal Nuclease. Detection and Localization by 1H NMR Spectroscopy of Conformational Changes Accompanying Substitutions for Glutamic Acid-43

Gerlt, J. A., Bolton, P. H., Hibler, D. W., Stolowich, N. J., Reynolds, M. A. & Wilde, J. A., Jan 1 1987, In : Biochemistry. 26, 19, p. 6278-6286 9 p.

Research output: Contribution to journalArticle

Micrococcal Nuclease
Nuclear magnetic resonance spectroscopy
Glutamic Acid
Substitution reactions
Magnetic Resonance Spectroscopy
1986

A method for the observation of selected proton NMR resonances of proteins

Wilde, J. A., Bolton, P. H., Stolowich, N. J. & Gerlt, J. A., Jun 1 1986, In : Journal of Magnetic Resonance (1969). 68, 1, p. 168-171 4 p.

Research output: Contribution to journalArticle

Identification of the Alkaline-Labile Product Accompanying Cytosine Release during Bleomycin-Mediated Degradation of d(CGCGCG)

Rabow, L., Stubbe, J., Kozarich, J. W. & Gerlt, J. A., Jan 1 1986, In : Journal of the American Chemical Society. 108, 22, p. 7130-7131 2 p.

Research output: Contribution to journalArticle

Cytosine
Bleomycin
Degradation
tri(deoxycytidylic acid-deoxyguanylic acid)
1985

The ompA signal peptide directed secretion of staphylococcal nuclease A by Escherichia coli

Takahara, M., Hibler, D. W., Barr, P. J., Gerlt, J. A. & Inouye, M., May 23 1985, In : Journal of Biological Chemistry. 260, 5, p. 2670-2674 5 p.

Research output: Contribution to journalArticle

Micrococcal Nuclease
Protein Sorting Signals
Escherichia coli
Genes
Periplasm

Thermal Denaturation of Staphylococcal Nuclease

Calderon, R. O., Stolowich, N. J., Gerlt, J. A. & Sturtevant, J. M., Oct 1 1985, In : Biochemistry. 24, 22, p. 6044-6049 6 p.

Research output: Contribution to journalArticle

Micrococcal Nuclease
Denaturation
Hot Temperature
Ligands
Enthalpy
1984

A General Procedure for Assigning the 31P Spectra of Nucleic Acids

Petersheim, M., Mehdi, S. & Gerlt, J. A., Jan 1 1984, In : Journal of the American Chemical Society. 106, 2, p. 439-440 2 p.

Research output: Contribution to journalArticle

Nucleic acids
Nucleic Acids
Deoxyribonuclease I
Thymidine
Phosphates
Phosphorus
Atoms
1983

15N and 17O NMR Studies of the Proton Binding Sites in Imidodiphosphate, Tetraethyl Imidodiphosphate, and Adenylyl Imidodiphosphate

Reynolds, M. A., Gerlt, J. A., Demou, P. C., Oppenheimer, N. J. & Kenyon, G. L., Jan 1 1983, In : Journal of the American Chemical Society. 105, 21, p. 6475-6481 7 p.

Research output: Contribution to journalArticle

Adenylyl Imidodiphosphate
Binding sites
Protonation
nuclear magnetic resonance
Protons

17O NMR as a Probe of Nucleic Acid Dynamics

Petersheim, M., Miner, V. W., Gerlt, J. A. & Prestegard, J. H., Jan 1 1983, In : Journal of the American Chemical Society. 105, 20, p. 6357-6358 2 p.

Research output: Contribution to journalArticle

Nucleic Acid Probes
Nucleic acids
Magnetic resonance
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance

17O NMR Spectral Properties of Pyrophosphate, Simple Phosphonates, and Thiophosphate and Phosphonate Analogues of ATP

Gerlt, J. A., Reynolds, M. A., Demou, P. C. & Kenyon, G. L., Jan 1 1983, In : Journal of the American Chemical Society. 105, 21, p. 6469-6475 7 p.

Research output: Contribution to journalArticle

Organophosphonates
Adenosinetriphosphate
Chemical shift
nuclear magnetic resonance
Adenosine Triphosphate

Evidence for a small catalytic domain in the adenylate cyclase from Salmonella typhimurium.

Leib, T. K. & Gerlt, J. A., Nov 10 1983, In : Journal of Biological Chemistry. 258, 21, p. 12982-12987 6 p.

Research output: Contribution to journalArticle

Salmonella
Salmonella typhimurium
Adenylyl Cyclases
Catalytic Domain
Peptides

Oxygen chiral phosphodiesters-8. Stereochemical course of the base-catalyzed hydrolysis of cyclic 2'-deoxyadenosine 3',5'-[17O, 18O]monophosphate

Mehdi, S., Coderre, J. A. & Gerlt, J. A., Jan 1 1983, In : Tetrahedron. 39, 21, p. 3483-3492 10 p.

Research output: Contribution to journalArticle

Hydrolysis
Oxygen
Reaction intermediates
Phosphorus
2'-deoxyadenosine
1982

17O NMR Spectral Properties of Simple Phosphate Esters and Adenine Nucleotides

Gerlt, J. A., Demou, P. C. & Mehdi, S., Jan 1 1982, In : Journal of the American Chemical Society. 104, 10, p. 2848-2856 9 p.

Research output: Contribution to journalArticle

Adenine Nucleotides
Chemical shift
Nucleotides
ester
nuclear magnetic resonance

Oxygen Chiral Phosphodiesters. 7. Stereochemical Course of a Reaction Catalyzed by Staphylococcal Nuclease

Mehdi, S. & Gerlt, J. A., Jan 1 1982, In : Journal of the American Chemical Society. 104, 11, p. 3223-3225 3 p.

Research output: Contribution to journalArticle

Micrococcal Nuclease
Oxygen
1981

Oxygen Chiral Phosphodiesters. 3. Use of 170 NMR Spectroscopy To Demonstrate Configurational Differences in the Diastereomers of Cyclic 2'-Deoxyadenosine 3’,5'-[17O,18O]Monophosphate

Coderre, J. A., Mehdi, S., Demou, P. C., Weber, R., Traficante, D. D. & Gerlt, J. A., Jan 1 1981, In : Journal of the American Chemical Society. 103, 7, p. 1870-1872 3 p.

Research output: Contribution to journalArticle

Nuclear magnetic resonance spectroscopy
Oxygen
2'-deoxyadenosine
Cyclic Nucleotides
Phosphoric Diester Hydrolases
Hydrolysis
Oxygen
cordycepin
Exonucleases
Thymidine
Hydrolysis
Phosphates
Oxygen
Thymidine
Hydrolysis
Phosphates
Biochemistry
Enzymes
1980

Conformational Properties of 5-Alkoxy and 5-Alkyl Substituted Trimethylene Phosphates in Solution

Gerlt, J. A., Gutterson, N. I., Drews, R. E. & Sokolow, J. A., Jan 1 1980, In : Journal of the American Chemical Society. 102, 5, p. 1665-1670 6 p.

Research output: Contribution to journalArticle

Phosphates
Cyclic AMP
phosphate
enthalpy
hydrolysis

Crystal and Molecular Structure of 5-Ethoxytrimethylenephosphoric Acid

Gerlt, J. A., Chodosh, D. F., Drews, R. E. & Adams, R. D., Jan 1 1980, In : Journal of Organic Chemistry. 45, 7, p. 1282-1286 5 p.

Research output: Contribution to journalArticle

Glycosides
Molecular structure
Crystal structure
Phosphates
Acids

Mechanism of the adenylate cyclase reaction. Stereochemistry of the reaction catalyzed by the enzyme from Brevibacterium liquefaciens

Gerlt, J. A., Coderre, J. A. & Wolin, M. S., Jan 1 1980, In : Journal of Biological Chemistry. 255, 2, p. 331-334 4 p.

Research output: Contribution to journalArticle

Thionucleotides
Brevibacterium
Magnetic resonance spectroscopy
Stereochemistry
Stereoisomerism
Fourier Analysis
Oxygen
2'-deoxy-5'-adenosine monophosphate
2'-deoxyadenosine
Thymidine
Esters
Phosphorus
phosphoramidic acid
4-nitrophenyl

Theoretical Calculations on the Geometric Destabilization of 3’,5'- and 2’,3'-Cyclic Nucleotides

Marsh, F. J., Weiner, P., KoIlman, P. A., Kenyon, G. L., Douglas, J. E. & Gerlt, J. A., Jan 1 1980, In : Journal of the American Chemical Society. 102, 5, p. 1660-1665 6 p.

Research output: Contribution to journalArticle

Cyclic Nucleotides
Cyclic AMP
hydrolysis
Hydrolysis
Phosphates

Thermochemical Identification of the Structural Factors Responsible for the Thermodynamic Instability of 3’,5'-Cyclic Nucleotides

Gerlt, J. A., Gutterson, N. I., Datta, P., Belleau, B. & Penney, C. L., Jan 1 1980, In : Journal of the American Chemical Society. 102, 5, p. 1655-1660 6 p.

Research output: Contribution to journalArticle

Cyclic Nucleotides
Thermodynamics
Phosphates
thermodynamics
phosphate

The Solution Conformational Preferences of the Sugar and Sugar Phosphate Constituents of RNA and DNA

Gerlt, J. A. & Virginia Youngblood, A., Jan 1 1980, In : Journal of the American Chemical Society. 102, 25, p. 7433-7438 6 p.

Research output: Contribution to journalArticle

Sugar Phosphates
Nucleotides
RNA
Nucleosides
Sugars
1979
Enterobacter aerogenes
Stereochemistry
Uridine
Cadmium
Phosphoric Monoester Hydrolases
1975

Purification and properties of a phosphohydrolase from Enterobacter aerogenes

Gerlt, J. A. & Whitman, G. J. R., 1975, In : Journal of Biological Chemistry. 250, 13, p. 5053-5058 6 p.

Research output: Contribution to journalArticle

4-Nitrophenylphosphatase
Magnetic resonance spectroscopy
Organophosphorus Compounds
Enterobacter aerogenes
Nitrophenols

The enthalpies of hydrolysis of acyclic, monocyclic, and glycoside cyclic phosphate diesters

Gerlt, J. A., Westheimer, F. H. & Sturtevant, J. M., 1975, In : Journal of Biological Chemistry. 250, 13, p. 5059-5067 9 p.

Research output: Contribution to journalArticle

Organophosphorus Compounds
Molecular Conformation
Ribonucleotides
Enterobacter
Cyclic Nucleotides
1973

The mechanism of action of 5' adenylic acid activated threonine dehydrase. IV. Characterization of kinetic effect of adenosine monophosphate

Dunne, C. P., Gerlt, J. A., Rabinowitz, K. W. & Wood, W. A., 1973, In : Journal of Biological Chemistry. 248, 23, p. 8189-8199 11 p.

Research output: Contribution to journalArticle

Threonine Dehydratase
Ultraviolet Spectrophotometry
Hydro-Lyases
Enzyme Activation
Adenine Nucleotides
Threonine Dehydratase
Ultraviolet Spectrophotometry
Allosteric Regulation
Hydro-Lyases
Enzyme Activation