John Alan Gerlt

If you made any changes in Pure these will be visible here soon.

Research Output

Filter
Article
2000

Evolution of enzymatic activities in the enolase superfamily: Crystallographic and mutagenesis studies of the reaction catalyzed by D- glucarate dehydratase from Escherichia coli

Gulick, A. M., Hubbard, B. K., Gerlt, J. A. & Rayment, I., Apr 25 2000, In : Biochemistry. 39, 16, p. 4590-4602 13 p.

Research output: Contribution to journalArticle

Evolution of enzymatic activity in the enolase superfamily: Structure of o-succinylbenzoate synthase from Escherichia coli in complex with Mg2+ and o-succinylbenzoate

Thompson, T. B., Garrett, J. B., Taylor, E. A., Meganathan, R., Gerlt, J. A. & Rayment, I., Sep 5 2000, In : Biochemistry. 39, 35, p. 10662-10676 15 p.

Research output: Contribution to journalArticle

New functions from old scaffolds: How nature reengineers enzymes for new functions

Babbit, P. C. & Gerlt, J. A., 2000, In : Advances in Protein Chemistry. 55, p. 1-28 28 p.

Research output: Contribution to journalArticle

New reactions in the crotonase superfamily: Structure of methylmalonyl CoA decarboxylase from Escherichia coli

Benning, M. M., Haller, T., Gerlt, J. A. & Holden, H. M., Apr 25 2000, In : Biochemistry. 39, 16, p. 4630-4639 10 p.

Research output: Contribution to journalArticle

1999

Evolution of enzymatic activities in the enolase superfamily: Identification of a 'new' general acid catalyst in the active site of D- galactonate dehydratase from Escherichia coli

Wieczorek, S. J., Kalivoda, K. A., Clifton, J. G., Ringe, D., Petsko, G. A. & Gerlt, J. A., May 12 1999, In : Journal of the American Chemical Society. 121, 18, p. 4540-4541 2 p.

Research output: Contribution to journalArticle

Unexpected divergence of enzyme function and sequence: 'N-acylamino acid racemase' is o-succinylbenzoate synthase

Palmer, D. R. J., Garrett, J. B., Sharma, V., Meganathan, R., Babbitt, P. C. & Gerlt, J. A., Apr 6 1999, In : Biochemistry. 38, 14, p. 4252-4258 7 p.

Research output: Contribution to journalArticle

1998

Evolution of an enzyme active site: The structure of a new crystal form of muconate lactonizing enzyme compared with mandelate racemase and enolase

Hasson, M. S., Schlichting, I., Moulai, J., Taylor, K., Barrett, W., Kenyon, G. L., Babbitt, P. C., Gerlt, J. A., Petsko, G. A. & Ringe, D., Sep 1 1998, In : Proceedings of the National Academy of Sciences of the United States of America. 95, 18, p. 10396-10401 6 p.

Research output: Contribution to journalArticle

Evolution of enzymatic activities in the enolase superfamily: Crystal structure of (D)-glucarate dehydratase from Pseudomonas putida

Gulick, A. M., Palmer, D. R. J., Babbitt, P. C., Gerlt, J. A. & Rayment, I., Oct 13 1998, In : Biochemistry. 37, 41, p. 14358-14368 11 p.

Research output: Contribution to journalArticle

Evolution of enzymatic activities in the enolase superfamily: Partitioning of reactive intermediates by (D)-glucarate dehydratase from Pseudomonas putida

Palmer, D. R. J., Hubbard, B. K. & Gerlt, J. A., Oct 13 1998, In : Biochemistry. 37, 41, p. 14350-14357 8 p.

Research output: Contribution to journalArticle

Evolution of enzymatic activities in the enolase superfamily: Characterization of the (D)-glucarate/galactarate catabolic pathway in Escherichia coli

Hubbard, B. K., Koch, M., Palmer, D. R. J., Babbitt, P. C. & Gerlt, J. A., Oct 13 1998, In : Biochemistry. 37, 41, p. 14369-14375 7 p.

Research output: Contribution to journalArticle

Mechanistically diverse enzyme superfamilies: The importance of chemistry in the evolution of catalysis

Gerlt, J. A. & Babbitt, P. C., Jan 1 1998, In : Current Opinion in Chemical Biology. 2, 5, p. 607-612 6 p.

Research output: Contribution to journalArticle

Structure and mechanism of benzoylformate decarboxylase, a thiamin dtthosphate-dependent enzyme

Kenvon, Q. L., Hasson, M. S., Muscate, A., McLeish, M. J., Harris, T. K., Babbitt, P. Ç., Gerlt, J. A., Petsko, G. A. & Rinye, D., Dec 1 1998, In : FASEB Journal. 12, 8, p. A1332

Research output: Contribution to journalArticle

The crystal structure of benzoylformate decarboxylase at 1.6 Å Resolution: Diversity of catalytic residues in thiamin diphosphate-dependent enzymes

Hasson, M. S., Muscate, A., McLeish, M. J., Polovnikova, L. S., Gerlt, J. A., Kenyon, G. L., Petsko, G. A. & Ringe, D., Jul 14 1998, In : Biochemistry. 37, 28, p. 9918-9930 13 p.

Research output: Contribution to journalArticle

Stereochemistry of the reaction catalyzed by galactonate dehydratase

Wieczorek, S. J., Mrachko, G. T. & Gerlt, J. A., Dec 1 1997, In : FASEB Journal. 11, 9, p. A1017

Research output: Contribution to journalArticle

The enolase superfamily: Different reactions catalyzed by similar active sites

Gerlt, J. A. & Babbitt, P. C., 1997, In : FASEB Journal. 11, 9

Research output: Contribution to journalArticle

1996

Enzyme-catalyzed enolization reactions: A theoretical study on the energetics of concerted and stepwise pathways

Sargent, A. L., Rollog, M. E., Almlöf, J. E., Gassman, P. G. & Gerlt, J. A., Dec 11 1996, In : Journal of Molecular Structure: THEOCHEM. 388, 1-3, p. 145-159 15 p.

Research output: Contribution to journalArticle

Mechanism of the reaction catalyzed by acetoacetate decarboxylase. Importance of lysine 116 in determining the pKa of active-site lysine 115

Highbarger, L. A., Gerlt, J. A. & Kenyon, G. L., Jan 9 1996, In : Biochemistry. 35, 1, p. 41-46 6 p.

Research output: Contribution to journalArticle

Mechanism of the reaction catalyzed by mandelate racemase: Structure and mechanistic properties of the D270N mutant

Schafer, S. L., Barrett, W. C., Kallarakal, A. T., Mitra, B., Kozarich, J. W., Gerlt, J. A., Clifton, J. G., Petsko, G. A. & Kenyon, G. L., May 7 1996, In : Biochemistry. 35, 18, p. 5662-5669 8 p.

Research output: Contribution to journalArticle

The enolase superfamily: A general strategy for enzyme-catalyzed abstraction of the α-protons of carboxylic acids

Babbitt, P. C., Hasson, M. S., Wedekind, J. E., Palmer, D. R. J., Barrett, W. C., Reed, G. H., Rayment, I., Ringe, D., Kenyon, G. L. & Gerlt, J. A., Dec 24 1996, In : Biochemistry. 35, 51, p. 16489-16501 13 p.

Research output: Contribution to journalArticle

1995

A functionally diverse enzyme superfamily than abstracts the α protons of carboxylic acids

Babbitt, P. C., Mrachko, G. T., Hasson, M. S., Huisman, G. W., Kolter, R., Ringe, D., Petsko, G. A., Kenyon, G. L. & Gerlt, J. A., 1995, In : Science. 267, 5201, p. 1159-1161 3 p.

Research output: Contribution to journalArticle

Mandelate Racemase: Structure-Function Studies of a Pseudosymmetric Enzyme

Kenyon, G. L., Gerlt, J. A., Petsko, G. A. & Kozarich, J. W., Jan 1 1995, In : Accounts of chemical research. 28, 4, p. 178-186 9 p.

Research output: Contribution to journalArticle

Mechanism of the Reaction Catalyzed by Mandelate Racemase: Importance of Electrophilic Catalysis by Glutamic Acid 317

Mitra, B., Kallarakal, A. T., Kozarich, J. W., Gerlt, J. A., Clifton, J. G., Petsko, G. A. & Kenyon, G. L., Mar 1995, In : Biochemistry. 34, 9, p. 2777-2787 11 p.

Research output: Contribution to journalArticle

Mechanism of the Reaction Catalyzed by Mandelate Racemase: Structure and Mechanistic Properties of the K166R Mutant

Kallarakal, A. T., Mitra, B., Kozarich, J. W., Gerlt, J. A., Clifton, J. G., Petsko, G. A. & Kenyon, G. L., Mar 1995, In : Biochemistry. 34, 9, p. 2788-2797 10 p.

Research output: Contribution to journalArticle

1994

Protein engineering to study enzyme catalytic mechanisms

Gerlt, J. A., 1994, In : Current Opinion in Structural Biology. 4, 4, p. 593-600 8 p.

Research output: Contribution to journalArticle

The Role of Lysine 166 in the Mechanism of Mandelate Racemase from Pseudomonas putida: Mechanistic and Crystallographic Evidence for Stereospecific Alkylation by (R)-α-Phenylglycidate

Landro, J. A., Gerlt, J. A., Kozarich, J. W., Koo, C. W., Shah, V. J., Kenyon, G. L., Neidhart, D. J., Fujita, S. & Petsko, G. A., Jan 1 1994, In : Biochemistry. 33, 3, p. 635-643 9 p.

Research output: Contribution to journalArticle

1993

A Novel Structural Basis for Membrane Association of a Protein: Construction of a Chimeric Soluble Mutant of (S)-Mandelate Dehydrogenase from Pseudomonas putida

Mitra, B., Gerlt, J. A., Babbitt, P. C., Koo, C. W., Kenyon, G. L., Joseph, D. & Petsko, G. A., 1993, In : Biochemistry. 32, 48, p. 12959-12967 9 p.

Research output: Contribution to journalArticle

Mechanism of the Reaction Catalyzed by Staphylococcal Nuclease: Identification of the Rate-Determining Step

Hale, S. P., Poole, L. B. & Gerlt, J. A., 1993, In : Biochemistry. 32, 29, p. 7479-7487 9 p.

Research output: Contribution to journalArticle

On the origin of enzymatic species

Petsko, G. A., Kenyon, G. L., Gerlt, J. A., Ringe, D. & Kozarich, J. W., Oct 1993, In : Trends in Biochemical Sciences. 18, 10, p. 372-376 5 p.

Research output: Contribution to journalArticle

The structural enzymology of proton-transfer reactions

Petsko, G., Ringe, D., Allen, K., Lavie, A., Gerhart-Mueller, E., Clifton, J., Hasson, M., Fujita, S., Sugio, S., Xhang, X., Davenport, R., Lolis, E., Neidhart, D., Kenyon, G., Gerlt, J., Bash, P., Knowles, J. & Karplus, M., Jan 1 1993, In : Protein Engineering, Design and Selection. 6, 1 p.

Research output: Contribution to journalArticle

1992

Mandelate racemase and class-related enzymes

Gerlt, J. A., Kenyon, G. L., Kozarich, J. W., Neidhart, D. J., Petsko, G. A. & Powers, V. M., Oct 1992, In : Current Opinion in Structural Biology. 2, 5, p. 736-742 7 p.

Research output: Contribution to journalArticle

Phosphate Ester Hydrolysis

Gerlt, J. A., Jan 1 1992, In : Enzymes. 20, C, p. 95-139 45 p.

Research output: Contribution to journalArticle

1991

Characterization of Conformational Features of DNA Heteroduplexes Containing Aldehydic Abasic Sites

Withka, J. M., Wilde, J. A., Bolton, P. H., Mazumder, A. & Gerlt, J. A., Oct 1 1991, In : Biochemistry. 30, 41, p. 9931-9940 10 p.

Research output: Contribution to journalArticle

Deletion of the Ω-Loop in the Active Site of Staphylococcal Nuclease. 1. Effect on Catalysis and Stability

Poole, L. B., Deborah Loveys, A., Hale, S. P., Gerlt, J. A., Stanczyk, S. M. & Bolton, P. H., Apr 1 1991, In : Biochemistry. 30, 15, p. 3621-3627 7 p.

Research output: Contribution to journalArticle

Deletion of the Ω-Loop in the Active Site of Staphylococcal Nuclease. 2. Effects on Protein Structure and Dynamics

Baldisseri, D. M., Torchia, D. A., Poole, L. B. & Gerlt, J. A., Apr 1 1991, In : Biochemistry. 30, 15, p. 3628-3633 6 p.

Research output: Contribution to journalArticle

Mechanism of the Reaction Catalyzed by Mandelate Racemase. 1. Chemical and Kinetic Evidence for a Two-Base Mechanism

Powers, V. M., Koo, C. W., Kenyon, G. L., Gerlt, J. A. & Kozarich, J. W., Sep 1 1991, In : Biochemistry. 30, 38, p. 9255-9263 9 p.

Research output: Contribution to journalArticle

Mechanism of the Reaction Catalyzed by Mandelate Racemase. 2. Crystal Structure of Mandelate Racemase at 2.5-Å Resolution: Identification of the Active Site and Possible Catalytic Residues

Neidhart, D. J., Howell, P. L., Petsko, G. A., Powers, V. M., Li, R., Kenyon, G. L. & Gerlt, J. A., Sep 1 1991, In : Biochemistry. 30, 38, p. 9264-9273 10 p.

Research output: Contribution to journalArticle

Mechanism of the Reaction Catalyzed by Mandelate Racemase. 3. Asymmetry in Reactions Catalyzed by the H297N Mutant

Landro, J. A., Kallarakal, A. T., Ransom, S. C., Gerlt, J. A., Kozarich, J. W., Neidhart, D. J. & Kenyon, G. L., Sep 1 1991, In : Biochemistry. 30, 38, p. 9274-9281 8 p.

Research output: Contribution to journalArticle

Stereochemical Studies of the β-Elimination Reactions at Aldehydic Abasic Sites in DNA: Endonuclease III from Escherichia coli, Sodium Hydroxide, and Lys-Trp-Lys

Mazumder, A., Gerlt, J. A., Absalon, M. J., Stubbe, J. A., Cunningham, R. P., Withka, J. & Bolton, P. H., Jan 1 1991, In : Biochemistry. 30, 4, p. 1119-1126 8 p.

Research output: Contribution to journalArticle

Structure of thialysine hydrochloride.

Ammon, H. L., Prasad, S. M. & Gerlt, J. A., Jul 15 1991, In : Acta crystallographica. Section C, Crystal structure communications. 47 ( Pt 7), p. 1476-1478 3 p.

Research output: Contribution to journalArticle

1990

Kinetic and Conformational Effects of Lysine Substitutions for Arginines 35 and 87 in the Active Site of Staphylococcal Nuclease

Pourmotabbed, T., Dell’Acqua, M., Gerlt, J. A., Stanczyk, S. M. & Bolton, P. H., Apr 1 1990, In : Biochemistry. 29, 15, p. 3677-3683 7 p.

Research output: Contribution to journalArticle

Mandelate Pathway of Pseudomonas putida: Sequence Relationships Involving Mandelate Racemase, (S)-Mandelate Dehydrogenase, and Benzoylformate Decarboxylase and Expression of Benzoylformate Decarboxylase in Escherichia coli

Tsou, A. Y., Ransom, S. C., Gerlt, J. A., Buechter, D. D., Babbitt, P. C. & Kenyon, G. L., Oct 1 1990, In : Biochemistry. 29, 42, p. 9856-9862 7 p.

Research output: Contribution to journalArticle

Mandelate racemase and muconate lactonizing enzyme are mechanistically distinct and structurally homologous

Neidhart, D. J., Kenyon, G. L., Gerlt, J. A. & Petsko, G. A., Jan 1 1990, In : Nature. 347, 6294, p. 692-694 3 p.

Research output: Contribution to journalArticle

Restructuring catalysis in the mandelate pathway

Neidhart, D. C., Howell, P. L., Petsko, G. A., Gerlt, J. A., Kozarich, J. W., Powers, V. M. & Kenyon, G. L., 1990, In : Biochemical Society Symposia. 57, p. 135-141 7 p.

Research output: Contribution to journalArticle

1989

Characterization of the Equilibrating Forms of the Aldehydic Abasic Site in Duplex DNA by 17O NMR

Wilde, J. A., Bolton, P. H., Mazumder, A., Manoharan, M. & Gerlt, J. A., Mar 1 1989, In : Journal of the American Chemical Society. 111, 5, p. 1894-1896 3 p.

Research output: Contribution to journalArticle

Isotopic labeling with hydrogen-2 and carbon-13 to compare conformations of proteins and mutants generated by site-directed mutagenesis, I

Hibler, D. W., Harpold, L., Dell'Acqua, M., Pourmotabbed, T., Gerlt, J. A., Wilde, J. A. & Bolton, P. H., Jan 1 1989, In : Methods in enzymology. 177, C, p. 74-86 13 p.

Research output: Contribution to journalArticle