John Alan Gerlt

1973 …2019
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Research Output 1973 2019

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2019

Discovery of novel bacterial queuine salvage enzymes and pathways in human pathogens

Yuan, Y., Zallot, R., Grove, T. L., Payan, D. J., Martin-Verstraete, I., Šepić, S., Balamkundu, S., Neelakandan, R., Gadi, V. K., Liu, C. F., Swairjo, M. A., Dedon, P. C., Almo, S. C., Gerlt, J. A. & de Crécy-Lagard, V., Sep 17 2019, In : Proceedings of the National Academy of Sciences of the United States of America. 116, 38, p. 19126-19135 10 p.

Research output: Contribution to journalArticle

Open Access
Bacteria
queuine tRNA-ribosyltransferase
Enzymes
Eukaryota
Nucleoside Q

Novel metabolic pathways and regulons for hexuronate utilization in Proteobacteria

Bouvier, J. T., Sernova, N. V., Ghasempur, S., Rodionova, I. A., Vetting, M. W., Al-Obaidi, N. F., Almo, S. C., Gerlt, J. A. & Rodionov, D. A., Jan 1 2019, In : Journal of bacteriology. 201, 2, e00431-18.

Research output: Contribution to journalArticle

Regulon
Proteobacteria
Metabolic Networks and Pathways
Ralstonia pickettii
Enzymes

The EFI Web Resource for Genomic Enzymology Tools: Leveraging Protein, Genome, and Metagenome Databases to Discover Novel Enzymes and Metabolic Pathways

Zallot, R., Oberg, N. & Gerlt, J. A., Oct 15 2019, In : Biochemistry. 58, 41, p. 4169-4182 14 p.

Research output: Contribution to journalArticle

Open Access
Metagenome
Metabolic Networks and Pathways
Genes
Genome
Databases
2018

Enzyme Architecture: Breaking Down the Catalytic Cage that Activates Orotidine 5′-Monophosphate Decarboxylase for Catalysis

Reyes, A. C., Plache, D. C., Koudelka, A. P., Amyes, T. L., Gerlt, J. A. & Richard, J. P., Dec 19 2018, In : Journal of the American Chemical Society. 140, 50, p. 17580-17590 11 p.

Research output: Contribution to journalArticle

Carboxy-Lyases
catalysis
Catalysis
Yeast
Enzymes

Functional assignment of multiple catabolic pathways for d-apiose article

Carter, M. S., Zhang, X., Huang, H., Bouvier, J. T., Francisco, B. S., Vetting, M. W., Al-Obaidi, N., Bonanno, J. B., Ghosh, A., Zallot, R. G., Andersen, H. M., Almo, S. C. & Gerlt, J. A., Jul 1 2018, In : Nature chemical biology. 14, 7, p. 696-705 10 p.

Research output: Contribution to journalArticle

Methylmalonyl-CoA carboxytransferase
Carrier Proteins
Phosphates
Genome
Pentoses

Prediction of enzymatic pathways by integrative pathway mapping

Calhoun, S., Korczynska, M., Wichelecki, D. J., San Francisco, B., Zhao, S., Rodionov, D. A., Vetting, M. W., Al-Obaidi, N. F., Lin, H., O’Meara, M. J., Scott, D. A., Morris, J. H., Russel, D., Almo, S. C., Osterman, A. L., Gerlt, J. A., Jacobson, M. P., Shoichet, B. K. & Sali, A., Jan 29 2018, In : eLife. 7, e31097.

Research output: Contribution to journalArticle

Metabolic Networks and Pathways
Enzymes
Crystallography
Metabolomics
Systems Biology
2017
Genes
Genome
Biological Products
Enzymes
Metabolic Networks and Pathways
2016

Assignment of function to a domain of unknown function: DUF1537 is a new kinase family in catabolic pathways for acid sugars

Zhang, X., Carter, M. S., Vetting, M. W., Francisco, B. S., Zhao, S., Al-Obaidi, N. F., Solbiati, J. O., Thiaville, J. J., Crécy-Lagard, V. D., Jacobson, M. P., Almo, S. C. & Gerlt, J. A., Jul 19 2016, In : Proceedings of the National Academy of Sciences of the United States of America. 113, 29, p. E4161-E4169

Research output: Contribution to journalArticle

Sugar Acids
Phosphotransferases
Carbon
Fructose-Bisphosphate Aldolase
Membrane Transport Proteins

Members of a Novel Kinase Family (DUF1537) Can Recycle Toxic Intermediates into an Essential Metabolite

Thiaville, J. J., Flood, J., Yurgel, S., Prunetti, L., Elbadawi-Sidhu, M., Hutinet, G., Forouhar, F., Zhang, X., Ganesan, V., Reddy, P., Fiehn, O., Gerlt, J. A., Hunt, J. F., Copley, S. D. & De Crécy-Lagard, V., Aug 19 2016, In : ACS chemical biology. 11, 8, p. 2304-2311 8 p.

Research output: Contribution to journalArticle

Pyridoxal Phosphate
Poisons
Metabolites
Phosphotransferases
Genes
Agrobacterium tumefaciens
Lactones
Crystallization
purification
Purification

Structure of an ABC transporter solute-binding protein specific for the amino sugars glucosamine and galactosamine

Yadava, U., Vetting, M. W., Al Obaidi, N., Carter, M. S., Gerlt, J. A. & Almo, S. C., Jan 1 2016, In : Acta Crystallographica Section:F Structural Biology Communications. 72, p. 467-472 6 p.

Research output: Contribution to journalArticle

Amino Sugars
transporter
Galactosamine
ATP-Binding Cassette Transporters
Glucosamine
2015

A General Strategy for the Discovery of Metabolic Pathways: D -Threitol, l -Threitol, and Erythritol Utilization in Mycobacterium smegmatis

Huang, H., Carter, M. S., Vetting, M. W., Al-Obaidi, N., Patskovsky, Y., Almo, S. C. & Gerlt, J. A., Nov 25 2015, In : Journal of the American Chemical Society. 137, 46, p. 14570-14573 4 p.

Research output: Contribution to journalArticle

Erythritol
Mycobacterium smegmatis
Metabolic Networks and Pathways
ATP-Binding Cassette Transporters
genome

ATP-binding cassette (ABC) transport system solute-binding protein-guided identification of novel D-altritol and galactitol catabolic pathways in Agrobacterium tumefaciens C58

Wichelecki, D. J., Vetting, M. W., Chou, L., Al-Obaidi, N., Bouvier, J. T., Almo, S. C. & Gerlt, J. A., Nov 27 2015, In : Journal of Biological Chemistry. 290, 48, p. 28963-28976 14 p.

Research output: Contribution to journalArticle

Galactitol
Agrobacterium tumefaciens
Carrier Proteins
Adenosine Triphosphate
Enzymes

A unique cis -3-hydroxy- l -proline dehydratase in the enolase superfamily

Zhang, X., Kumar, R., Vetting, M. W., Zhao, S., Jacobson, M. P., Almo, S. C. & Gerlt, J. A., Feb 4 2015, In : Journal of the American Chemical Society. 137, 4, p. 1388-1391 4 p.

Research output: Contribution to journalArticle

Hydroxyproline
Hydro-Lyases
Phosphopyruvate Hydratase
Proline
dehydration

Experimental strategies for functional annotation and metabolism discovery: Targeted screening of solute binding proteins and unbiased panning of metabolomes

Vetting, M. W., Al-Obaidi, N., Zhao, S., San Francisco, B., Kim, J., Wichelecki, D. J., Bouvier, J. T., Solbiati, J. O., Vu, H., Zhang, X., Rodionov, D. A., Love, J. D., Hillerich, B. S., Seidel, R. D., Quinn, R. J., Osterman, A. L., Cronan, J. E., Jacobson, M. P., Gerlt, J. A. & Almo, S. C., Jan 27 2015, In : Biochemistry. 54, 3, p. 909-931 23 p.

Research output: Contribution to journalArticle

Metabolome
Metabolism
Carrier Proteins
Screening
Libraries

Integration of untargeted metabolomics with transcriptomics reveals active metabolic pathways

Cho, K., Evans, B. S., Wood, B. M. K., Kumar, R., Erb, T. J., Warlick, B. P., Gerlt, J. A. & Sweedler, J. V., Jun 1 2015, In : Metabolomics. 11, 3, p. 503-517 15 p.

Research output: Contribution to journalArticle

Metabolomics
Metabolic Networks and Pathways
Salvaging
Methionine
Metabolites

Rate and Equilibrium Constants for an Enzyme Conformational Change during Catalysis by Orotidine 5′-Monophosphate Decarboxylase

Goryanova, B., Goldman, L. M., Ming, S., Amyes, T. L., Gerlt, J. A. & Richard, J. P., Jul 28 2015, In : Biochemistry. 54, 29, p. 4555-4564 10 p.

Research output: Contribution to journalArticle

Decarboxylation
Carboxy-Lyases
Equilibrium constants
Catalysis
Rate constants
2014

Discovery of a novel l -lyxonate degradation pathway in Pseudomonas aeruginosa PAO1

Ghasempur, S., Eswaramoorthy, S., Hillerich, B. S., Seidel, R. D., Swaminathan, S., Almo, S. C. & Gerlt, J. A., May 27 2014, In : Biochemistry. 53, 20, p. 3357-3366 10 p.

Research output: Contribution to journalArticle

Hydro-Lyases
Pseudomonas aeruginosa
mandelate racemase
Degradation
Dehydration

Discovery of function in the enolase superfamily: D-mannonate and d-gluconate dehydratases in the d-mannonate dehydratase subgroup

Wichelecki, D. J., Balthazor, B. M., Chau, A. C., Vetting, M. W., Fedorov, A. A., Fedorov, E. V., Lukk, T., Patskovsky, Y. V., Stead, M. B., Hillerich, B. S., Seidel, R. D., Almo, S. C. & Gerlt, J. A., Apr 29 2014, In : Biochemistry. 53, 16, p. 2722-2731 10 p.

Research output: Contribution to journalArticle

gluconate dehydratase
Phosphopyruvate Hydratase
Dehydration
mandelate racemase
Sugar Acids

Enzymatic and structural characterization of rTSγ provides insights into the function of rTSβ

Wichelecki, D. J., Froese, D. S., Kopec, J., Muniz, J. R. C., Yue, W. W. & Gerlt, J. A., Apr 29 2014, In : Biochemistry. 53, 16, p. 2732-2738 7 p.

Research output: Contribution to journalArticle

Thymidylate Synthase
Protein Isoforms
Gene encoding
Hydro-Lyases
Biosynthesis

Enzyme architecture: Deconstruction of the enzyme-activating phosphodianion interactions of orotidine 5′-monophosphate decarboxylase

Goldman, L. M., Amyes, T. L., Goryanova, B., Gerlt, J. A. & Richard, J. P., Jul 16 2014, In : Journal of the American Chemical Society. 136, 28, p. 10156-10165 10 p.

Research output: Contribution to journalArticle

Carboxy-Lyases
Binding energy
Enzymes
enzyme
Grippers

Evolution of enzymatic activities in the enolase superfamily: Galactarate dehydratase III from agrobacterium tumefaciens C58

Groninger-Poe, F. P., Bouvier, J. T., Vetting, M. W., Kalyanaraman, C., Kumar, R., Almo, S. C., Jacobson, M. P. & Gerlt, J. A., Jul 1 2014, In : Biochemistry. 53, 25, p. 4192-4203 12 p.

Research output: Contribution to journalArticle

Hydro-Lyases
Agrobacterium tumefaciens
Phosphopyruvate Hydratase
Sugar Acids
Biochemistry

Galactaro δ-lactone isomerase: Lactone isomerization by a member of the amidohydrolase superfamily

Bouvier, J. T., Groninger-Poe, F. P., Vetting, M., Almo, S. C. & Gerlt, J. A., Feb 4 2014, In : Biochemistry. 53, 4, p. 614-616 3 p.

Research output: Contribution to journalArticle

Amidohydrolases
Isomerases
Lactones
Isomerization
4 alpha-glucanotransferase

Identification of the in vivo function of the high-efficiency d -mannonate dehydratase in caulobacter crescentus NA1000 from the enolase superfamily

Wichelecki, D. J., Graff, D. C., Al-Obaidi, N., Almo, S. C. & Gerlt, J. A., Jul 1 2014, In : Biochemistry. 53, 25, p. 4087-4089 3 p.

Research output: Contribution to journalArticle

Caulobacter crescentus
Phosphopyruvate Hydratase
Glucuronic Acid
Biochemistry
Metabolism

Investigating the physiological roles of low-efficiency D-mannonate and D-gluconate dehydratases in the enolase superfamily: Pathways for the catabolism of L-gulonate and L-idonate

Wichelecki, D. J., Vendiola, J. A. F., Jones, A. M., Al-Obaidi, N., Almo, S. C. & Gerlt, J. A., Sep 9 2014, In : Biochemistry. 53, 35, p. 5692-5699 8 p.

Research output: Contribution to journalArticle

gluconate dehydratase
Phosphopyruvate Hydratase
Dehydration
Biochemistry
Chromohalobacter

Investigating the role of a backbone to substrate hydrogen bond in OMP decarboxylase using a site-specific amide to ester substitution

Desai, B. J., Goto, Y., Cembran, A., Fedorov, A. A., Almo, S. C., Gao, J., Suga, H. & Gerlt, J. A., Oct 21 2014, In : Proceedings of the National Academy of Sciences of the United States of America. 111, 42, p. 15066-15071 6 p.

Research output: Contribution to journalArticle

Carboxy-Lyases
Amides
Hydrogen
Esters
Amber

Prediction and biochemical demonstration of a catabolic pathway for the osmoprotectant proline betaine

Kumar, R., Zhao, S., Vetting, M. W., Wood, B. M. K., Sakai, A., Cho, K., Solbiati, J., Almo, S. C., Sweedler, J. V., Jacobson, M. P., Gerlt, J. A. & Cronan, J. E., Feb 11 2014, In : mBio. 5, 1, e00933-13.

Research output: Contribution to journalArticle

Betaine
Paracoccus denitrificans
Metabolomics
Hydroxyproline
Rhodobacter sphaeroides

Prediction and characterization of enzymatic activities guided by sequence similarity and genome neighborhood networks

Zhao, S., Sakai, A., Zhang, X., Vetting, M. W., Kumar, R., Hillerich, B., Francisco, B. S., Solbiati, J., Steves, A., Brown, S., Akiva, E., Barber, A., Seidel, R. D., Babbitt, P. C., Almo, S. C., Gerlt, J. A. & Jacobson, M. P., Jun 30 2014, In : eLife. 2014, 3, 03275.

Research output: Contribution to journalArticle

Metabolic Networks and Pathways
Genes
Genome
Enzymes
Archaea

Prediction and characterization of enzymatic activities guided by sequence similarity and genome neighborhood networks

Zhao, S., Sakai, A., Zhang, X., Vetting, M. W., Kumar, R., Hillerich, B., San Francisco, B., Solbiati, J., Steves, A., Brown, S., Akiva, E., Barber, A., Seidel, R. D., Babbitt, P. C., Almo, S. C., Gerlt, J. A. & Jacobson, M. P., Jan 1 2014, In : eLife. 3

Research output: Contribution to journalArticle

Metabolic Networks and Pathways
Genes
Genome
Enzymes
Archaea

Roles of small laccases from streptomyces in lignin degradation

Majumdar, S., Lukk, T., Solbiati, J. O., Bauer, S., Nair, S. K., Cronan, J. E. & Gerlt, J. A., Jun 24 2014, In : Biochemistry. 53, 24, p. 4047-4058 12 p.

Research output: Contribution to journalArticle

Laccase
Lignin
Streptomyces
Degradation
Streptomyces coelicolor
2013

Computational, structural, and kinetic evidence that Vibrio vulnificus FrsA is not a cofactor-independent pyruvate decarboxylase

Kellett, W. F., Brunk, E., Desai, B. J., Fedorov, A. A., Almo, S. C., Gerlt, J. A., Rothlisberger, U. & Richards, N. G. J., Mar 19 2013, In : Biochemistry. 52, 11, p. 1842-1844 3 p.

Research output: Contribution to journalArticle

Pyruvate Decarboxylase
Vibrio vulnificus
Enzyme kinetics
Fermentation
Respiration

Discovery of new enzymes and metabolic pathways by using structure and genome context

Zhao, S., Kumar, R., Sakai, A., Vetting, M. W., Wood, B. M. K., Brown, S., Bonanno, J. B., Hillerich, B. S., Seidel, R. D., Babbitt, P. C., Almo, S. C., Sweedler, J. V., Gerlt, J. A., Cronan, J. E. & Jacobson, M. P., Sep 24 2013, In : Nature. 502, 7473, p. 698-702 5 p.

Research output: Contribution to journalArticle

Metabolic Networks and Pathways
Genome
Multigene Family
Substrate Specificity
Enzymes

Role of a guanidinium cation-phosphodianion pair in stabilizing the vinyl carbanion intermediate of orotidine 5′-phosphate decarboxylase-catalyzed reactions

Goryanova, B., Goldman, L. M., Amyes, T. L., Gerlt, J. A. & Richard, J. P., Nov 6 2013, In : Biochemistry. 52, 42, p. 7500-7511 12 p.

Research output: Contribution to journalArticle

Orotidine-5'-Phosphate Decarboxylase
Guanidine
Cations
Stabilization
Decarboxylation
2012

1-methylthio-d-xylulose 5-phosphate methylsulfurylase: A novel route to 1-deoxy-d-xylulose 5-phosphate in Rhodospirillum rubrum

Warlick, B. P., Evans, B. S., Erb, T. J., Ramagopal, U. A., Sriram, J., Imker, H. J., Sauder, J. M., Bonanno, J. B., Burley, S. K., Tabita, F. R., Almo, S. C., Sweedler, J. V. & Gerlt, J. A., Oct 23 2012, In : Biochemistry. 51, 42, p. 8324-8326 3 p.

Research output: Contribution to journalArticle

Rhodospirillum rubrum
Salvaging
S-Adenosylmethionine
Biosynthesis
Terpenes

A RubisCO-like protein links SAM metabolism with isoprenoid biosynthesis

Erb, T. J., Evans, B. S., Cho, K., Warlick, B. P., Sriram, J., Wood, B. M. K., Imker, H. J., Sweedler, J. V., Tabita, F. R. & Gerlt, J. A., Nov 2012, In : Nature chemical biology. 8, 11, p. 926-932 7 p.

Research output: Contribution to journalArticle

Oxygenases
Terpenes
Rhodospirillum rubrum
S-Adenosylmethionine
Metabolomics

Conformational changes in orotidine 5′-monophosphate decarboxylase: A structure-based explanation for how the 5′-phosphate group activates the enzyme

Desai, B. J., Wood, B. M. K., Fedorov, A. A., Fedorov, E. V., Goryanova, B., Amyes, T. L., Richard, J. P., Almo, S. C. & Gerlt, J. A., Oct 30 2012, In : Biochemistry. 51, 43, p. 8665-8678 14 p.

Research output: Contribution to journalArticle

Carboxy-Lyases
Binding energy
Phosphates
Substrates
Enzymes

Homology models guide discovery of diverse enzyme specificities among dipeptide epimerases in the enolase superfamily

Lukk, T., Sakai, A., Kalyanaraman, C., Brown, S. D., Imker, H. J., Song, L., Fedorov, A. A., Fedorov, E. V., Toro, R., Hillerich, B., Seidel, R., Patskovsky, Y., Vetting, M. W., Nair, S. K., Babbitt, P. C., Almo, S. C., Gerlt, J. A. & Jacobson, M. P., Mar 13 2012, In : Proceedings of the National Academy of Sciences of the United States of America. 109, 11, p. 4122-4127 6 p.

Research output: Contribution to journalArticle

Racemases and Epimerases
Phosphopyruvate Hydratase
Dipeptides
Enzymes
alanylglutamic acid

Mechanistic diversity in the RuBisCO superfamily: RuBisCO from rhodospirillum rubrum is not promiscuous for reactions catalyzed by RuBisCO-like proteins

Warlick, B. P. E., Imker, H. J., Sriram, J., Tabita, F. R. & Gerlt, J. A., Nov 27 2012, In : Biochemistry. 51, 47, p. 9470-9479 10 p.

Research output: Contribution to journalArticle

Rhodospirillum rubrum
Carboxylation
Isomerases
Oxygenases
Phosphopyruvate Hydratase

Orotidine 5′-monophosphate decarboxylase: Transition state stabilization from remote protein-phosphodianion interactions

Amyes, T. L., Ming, S. A., Goldman, L. M., Wood, B. M., Desai, B. J., Gerlt, J. A. & Richard, J. P., Jun 12 2012, In : Biochemistry. 51, 23, p. 4630-4632 3 p.

Research output: Contribution to journalArticle

Decarboxylation
Carboxy-Lyases
Stabilization
Orotic Acid
Phosphites

Proton transfer from C-6 of uridine 5′-monophosphate catalyzed by orotidine 5′-monophosphate decarboxylase: Formation and stability of a vinyl carbanion intermediate and the effect of a 5-fluoro substituent

Tsang, W. Y., Wood, B. M. K., Wong, F. M., Wu, W., Gerlt, J. A., Amyes, T. L. & Richard, J. P., Sep 5 2012, In : Journal of the American Chemical Society. 134, 35, p. 14580-14594 15 p.

Research output: Contribution to journalArticle

Uridine Monophosphate
Proton transfer
Carboxy-Lyases
deuterium
Deuterium
2011

Mechanism of the orotidine 5′-monophosphate decarboxylase-catalyzed reaction: Importance of residues in the orotate binding site

Iiams, V., Desai, B. J., Fedorov, A. A., Fedorov, E. V., Almo, S. C. & Gerlt, J. A., Oct 4 2011, In : Biochemistry. 50, 39, p. 8497-8507 11 p.

Research output: Contribution to journalArticle

Carboxy-Lyases
Cats
Binding Sites
Substitution reactions
Energy barriers

OMP decarboxylase: Phosphodianion binding energy is used to stabilize a vinyl carbanion intermediate

Goryanova, B., Amyes, T. L., Gerlt, J. A. & Richard, J. P., May 4 2011, In : Journal of the American Chemical Society. 133, 17, p. 6545-6548 4 p.

Research output: Contribution to journalArticle

Orotidine-5'-Phosphate Decarboxylase
Deuterium
deuterium
Binding energy
Phosphites
2010

Activation of R235A mutant orotidine 5′-monophosphate decarboxylase by the guanidinium cation: Effective molarity of the cationic side chain of Arg-235

Barnett, S. A., Amyes, T. L., Wood, B. M., Gerlt, J. A. & Richard, J. P., Feb 9 2010, In : Biochemistry. 49, 5, p. 824-826 3 p.

Research output: Contribution to journalArticle

Carboxy-Lyases
Guanidine
Cations
Chemical activation
Decarboxylation

Conformational changes in orotidine 5′-monophosphate decarboxylase: "remote" residues that stabilize the active conformation

Wood, B. M. K., Amyes, T. L., Fedorov, A. A., Fedorov, E. V., Shabila, A., Almo, S. C., Richard, J. P. & Gerlt, J. A., May 4 2010, In : Biochemistry. 49, 17, p. 3514-3516 3 p.

Research output: Contribution to journalArticle

Carboxy-Lyases
Conformations
Catalytic Domain
Clamping devices
Binding energy
Reaction intermediates
Carboxy-Lyases
Deuterium
deuterium
decarboxylation
2009

An examination of the relationship between active site loop size and thermodynamic activation parameters for orotidine 5′-monophosphate decarboxylase from mesophilic and thermophilic organisms

Toth, K., Amyes, T. L., Wood, B. M., Chan, K. K., Gerlt, J. A. & Richard, J. P., Aug 25 2009, In : Biochemistry. 48, 33, p. 8006-8013 8 p.

Research output: Contribution to journalArticle

Carboxy-Lyases
Thermodynamics
Decarboxylation
Catalytic Domain
Chemical activation

Computation-facilitated assignment of the function in the enolase superfamily: A regiochemically distinct galactarate dehydratase from Oceanobacillus iheyensis

Rakus, J. F., Kalyanaraman, C., Fedorov, A. A., Fedorov, E. V., Mills-Groninger, F. P., Toro, R., Bonanno, J., Bain, K., Sauder, J. M., Burley, S. K., Almo, S. C., Jacobson, M. P. & Gerlt, J. A., Dec 8 2009, In : Biochemistry. 48, 48, p. 11546-11558 13 p.

Research output: Contribution to journalArticle

Hydro-Lyases
Phosphopyruvate Hydratase
Sugars
Catalytic Domain
Sugar Acids

Evolution of enzymatic activities in the enolase superfamily: Stereochemically distinct mechanisms in two families of cis,cis-muconate lactonizing enzymes

Sakai, A., Fedorov, A. A., Fedorov, E. V., Schnoes, A. M., Glasner, M. E., Brown, S., Rutter, M. E., Bain, K., Chang, S., Gheyi, T., Sauder, J. M., Burley, S. K., Babbitt, P. C., Almo, S. C. & Gerlt, J. A., Feb 24 2009, In : Biochemistry. 48, 7, p. 1445-1453 9 p.

Research output: Contribution to journalArticle

muconate cycloisomerase
Phosphopyruvate Hydratase
Substrate Specificity
Mycobacterium smegmatis
Pseudomonas fluorescens

Mechanism of the orotidine 5′-monophosphate decarboxylase-catalyzed reaction: Effect of solvent viscosity on kinetic constants

Wood, B. M. K., Chan, K. K., Amyes, T. L., Richard, J. P. & Gerlt, J. A., Jun 23 2009, In : Biochemistry. 48, 24, p. 5510-5517 8 p.

Research output: Contribution to journalArticle

Carboxy-Lyases
Viscosity
Decarboxylation
Kinetics
Substrates

Mechanism of the orotidine 5′-monophosphate decarboxylase-catalyzed reaction: Evidence for substrate destabilization

Chan, K. K., Wood, B. M. K., Fedorov, A. A., Fedorov, E. V., Imker, H. J., Amyes, T. L., Richard, J. P., Almo, S. C. & Gerlt, J. A., Jun 23 2009, In : Biochemistry. 48, 24, p. 5518-5531 14 p.

Research output: Contribution to journalArticle

Decarboxylation
Carboxy-Lyases
Deuterium
Catalytic Domain
Methanobacteriaceae