Douglas Alan Mitchell

2024

Biosynthesis of Macrocyclic Peptides with C‑Terminal β‑Amino-α-keto Acid Groups by Three Different Metalloenzymes

Nguyen, D. T., Zhu, L., Gray, D. L., Woods, T. J., Padhi, C., Flatt, K. M., Mitchell, D. A. & van der Donk, W. A., May 22 2024, In: ACS Central Science. 10, 5, p. 1022-1032 11 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Darobactin Substrate Engineering and Computation Show Radical Stability Governs Ether versus C-C Bond Formation

Woodard, A. M., Peccati, F., Navo, C. D., Jiménez-Osés, G. & Mitchell, D. A., May 22 2024, In: Journal of the American Chemical Society. 146, 20, p. 14328-14340 13 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Discovery of Borosin Catalytic Strategies and Function through Bioinformatic Profiling

Lee, A. R., Carter, R. S., Imani, A. S., Dommaraju, S. R., Hudson, G. A., Mitchell, D. A. & Freeman, M. F., May 17 2024, In: ACS chemical biology. 19, 5, p. 1116-1124 9 p.

Research output: Contribution to journal › Article › peer-review

Enterolyin S, a Polythiazole-containing Hemolytic Peptide from Enterococcus caccae

Shi, C., Patel, V. A., Mitchell, D. A. & Zhao, H., Jun 17 2024, In: ChemBioChem. 25, 12, e202400212.

Research output: Contribution to journal › Article › peer-review

Open Access

High-Yield Lasso Peptide Production in a Burkholderia Bacterial Host by Plasmid Copy Number Engineering

Fernandez, H. N., Kretsch, A. M., Kunakom, S., Kadjo, A. E., Mitchell, D. A. & Eustáquio, A. S., Jan 19 2024, In: ACS synthetic biology. 13, 1, p. 337-350 14 p.

Research output: Contribution to journal › Article › peer-review

Non-modular fatty acid synthases yield distinct N-terminal acylation in ribosomal peptides

Ren, H., Huang, C., Pan, Y., Dommaraju, S. R., Cui, H., Li, M., Gadgil, M. G., Mitchell, D. A. & Zhao, H., Aug 2024, In: Nature Chemistry. 16, 8, p. 1320-1329 10 p.

Research output: Contribution to journal › Article › peer-review

Tryptophan-Centric Bioinformatics Identifies New Lasso Peptide Modifications

Harris, L. A., Saad, H., Shelton, K. E., Zhu, L., Guo, X. & Mitchell, D. A., Apr 2 2024, In: Biochemistry. 63, 7, p. 865-879 15 p.

Research output: Contribution to journal › Article › peer-review

2023

Bioinformatics-guided discovery of biaryl-linked lasso peptides

Saad, H., Majer, T., Bhattarai, K., Lampe, S., Nguyen, D. T., Kramer, M., Straetener, J., Brötz-Oesterhelt, H., Mitchell, D. A. & Gross, H., Oct 30 2023, In: Chemical Science. 14, 45, p. 13176-13183 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Catalytic Site Proximity Profiling for Functional Unification of Sequence-Diverse Radical S-Adenosylmethionine Enzymes

Precord, T. W., Ramesh, S., Dommaraju, S. R., Harris, L. A., Kille, B. L. & Mitchell, D. A., Jun 21 2023, In: ACS Bio and Med Chem Au. 3, 3, p. 240-251 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Genome mining unveils a class of ribosomal peptides with two amino termini

Ren, H., Dommaraju, S. R., Huang, C., Cui, H., Pan, Y., Nesic, M., Zhu, L., Sarlah, D., Mitchell, D. A. & Zhao, H., Dec 2023, In: Nature communications. 14, 1, 1624.

Research output: Contribution to journal › Article › peer-review

Open Access

McrD binds asymmetrically to methyl-coenzyme M reductase improving active-site accessibility during assembly

Chadwick, G. L., Joiner, A. M. N., Ramesh, S., Mitchell, D. A. & Nayak, D. D., Jun 20 2023, In: Proceedings of the National Academy of Sciences of the United States of America. 120, 25, e2302815120.

Research output: Contribution to journal › Article › peer-review

Open Access

Peptidase Activation by a Leader Peptide-Bound RiPP Recognition Element

Kretsch, A. M., Gadgil, M. G., Dicaprio, A. J., Barrett, S. E., Kille, B. L., Si, Y., Zhu, L. & Mitchell, D. A., Feb 21 2023, In: Biochemistry. 62, 4, p. 956-967 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

2022

Accessing Diverse Pyridine-Based Macrocyclic Peptides by a Two-Site Recognition Pathway

Nguyen, D. T., Le, T. T., Rice, A. J., Hudson, G. A., Van Der Donk, W. A. & Mitchell, D. A., Jun 29 2022, In: Journal of the American Chemical Society. 144, 25, p. 11263-11269 7 p.

Research output: Contribution to journal › Article › peer-review

Open Access

A scalable platform to discover antimicrobials of ribosomal origin

Ayikpoe, R. S., Shi, C., Battiste, A. J., Eslami, S. M., Ramesh, S., Simon, M. A., Bothwell, I. R., Lee, H., Rice, A. J., Ren, H., Tian, Q., Harris, L. A., Sarksian, R., Zhu, L., Frerk, A. M., Precord, T. W., van der Donk, W. A., Mitchell, D. A. & Zhao, H., Dec 2022, In: Nature communications. 13, 1, 6135.

Research output: Contribution to journal › Article › peer-review

Open Access

Enzymatic Pyridine Aromatization during Thiopeptide Biosynthesis

Rice, A. J., Pelton, J. M., Kramer, N. J., Catlin, D. S., Nair, S. K., Pogorelov, T. V., Mitchell, D. A. & Bowers, A. A., Nov 23 2022, In: Journal of the American Chemical Society. 144, 46, p. 21116-21124 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

2021

Bioinformatics-Guided Expansion and Discovery of Graspetides

Ramesh, S., Guo, X., DiCaprio, A. J., De Lio, A. M., Harris, L. A., Kille, B. L., Pogorelov, T. V. & Mitchell, D. A., Dec 17 2021, In: ACS chemical biology. 16, 12, p. 2787-2797 11 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Cell-Free Biosynthesis to Evaluate Lasso Peptide Formation and Enzyme-Substrate Tolerance

Si, Y., Kretsch, A. M., Daigh, L. M., Burk, M. J. & Mitchell, D. A., Apr 21 2021, In: Journal of the American Chemical Society. 143, 15, p. 5917-5927 11 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Functional elucidation of TfuA in peptide backbone thioamidation

Liu, A., Si, Y., Dong, S. H., Mahanta, N., Penkala, H. N., Nair, S. K. & Mitchell, D. A., May 2021, In: Nature chemical biology. 17, 5, p. 585-592 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Sterol Sponge Mechanism Is Conserved for Glycosylated Polyene Macrolides

Guo, X., Zhang, J., Li, X., Xiao, E., Lange, J. D., Rienstra, C. M., Burke, M. D. & Mitchell, D. A., May 26 2021, In: ACS Central Science. 7, 5, p. 781-791 11 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Structure Prediction and Synthesis of Pyridine-Based Macrocyclic Peptide Natural Products

Hudson, G. A., Hooper, A. R., Dicaprio, A. J., Sarlah, D. & Mitchell, D. A., Jan 15 2021, In: Organic Letters. 23, 2, p. 253-256 4 p.

Research output: Contribution to journal › Article › peer-review

Open Access

2020

Bioinformatic and reactivity-based discovery of linaridins

Georgiou, M. A., Dommaraju, S. R., Guo, X., Mast, D. H. & Mitchell, D. A., Nov 20 2020, In: ACS chemical biology. 15, 11, p. 2976-2985 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Functional interactions between posttranslationally modified amino acids of methyl-coenzyme M reductase in Methanosarcina acetivorans

Nayak, D. D., Liu, A., Agrawal, N., Rodriguez-Carerro, R., Dong, S. H., Mitchell, D. A., Nair, S. K. & Metcalf, W. W., Feb 2020, In: PLoS biology. 18, 2, e3000507.

Research output: Contribution to journal › Article › peer-review

Open Access

Microviridin 1777: A Toxic Chymotrypsin Inhibitor Discovered by a Metabologenomic Approach

Sieber, S., Grendelmeier, S. M., Harris, L. A., Mitchell, D. A. & Gademann, K., Feb 28 2020, In: Journal of Natural Products. 83, 2, p. 438-446 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Precursor peptide-targeted mining of more than one hundred thousand genomes expands the lanthipeptide natural product family

Walker, M. C., Eslami, S. M., Hetrick, K. J., Ackenhusen, S. E., Mitchell, D. A. & Van Der Donk, W. A., Jun 3 2020, In: BMC genomics. 21, 1, 387.

Research output: Contribution to journal › Article › peer-review

Open Access

Reactivity-Based Screening for Citrulline-Containing Natural Products Reveals a Family of Bacterial Peptidyl Arginine Deiminases

Harris, L. A., Saint-Vincent, P. M. B., Guo, X., Hudson, G. A., Dicaprio, A. J., Zhu, L. & Mitchell, D. A., Dec 18 2020, In: ACS chemical biology. 15, 12, p. 3167-3175 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

RRE-Finder: A genome-mining tool for class-independent RiPP discovery

Kloosterman, A. M., Shelton, K. E., van Wezel, G. P., Medema, M. H. & Mitchell, D. A., Oct 2020, In: mSystems. 5, 5, e0026720.

Research output: Contribution to journal › Article › peer-review

Open Access

2019

Bioinformatic mapping of radical S-adenosylmethionine-dependent ribosomally synthesized and post-translationally modified peptides identifies new Cα, Cβ, and Cγ-linked thioether-containing peptides

Hudson, G. A., Burkhart, B. J., DiCaprio, A. J., Schwalen, C. J., Kille, B., Pogorelov, T. V. & Mitchell, D. A., 2019, In: Journal of the American Chemical Society. 141, 20, p. 8228-8238 11 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Enzymatic Reconstitution and Biosynthetic Investigation of the Lasso Peptide Fusilassin

Dicaprio, A. J., Firouzbakht, A., Hudson, G. A. & Mitchell, D. A., Jan 9 2019, In: Journal of the American Chemical Society. 141, 1, p. 290-297 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Mechanistic Basis for Ribosomal Peptide Backbone Modifications

Dong, S. H., Liu, A., Mahanta, N., Mitchell, D. A. & Nair, S. K., May 22 2019, In: ACS Central Science. 5, 5, p. 842-851 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Reconstitution and Substrate Specificity of the Thioether-Forming Radical S-Adenosylmethionine Enzyme in Freyrasin Biosynthesis

Precord, T. W., Mahanta, N. & Mitchell, D. A., Sep 20 2019, In: ACS chemical biology. 14, 9, p. 1981-1989 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

2018

Bioinformatic Expansion and Discovery of Thiopeptide Antibiotics

Schwalen, C. J., Hudson, G. A., Kille, B. & Mitchell, D. A., Aug 1 2018, In: Journal of the American Chemical Society. 140, 30, p. 9494-9501 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Elucidation of the roles of conserved residues in the biosynthesis of the lasso peptide paeninodin

Hegemann, J. D., Schwalen, C. J., Mitchell, D. A. & Van der Donk, W. A., 2018, In: Chemical Communications. 54, 65, p. 9007-9010 4 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Enzymatic reconstitution of ribosomal peptide backbone thioamidation

Mahanta, N., Liu, A., Dong, S., Nair, S. K. & Mitchell, D. A., Mar 20 2018, In: Proceedings of the National Academy of Sciences of the United States of America. 115, 12, p. 3030-3035 6 p.

Research output: Contribution to journal › Article › peer-review

Open Access

2017

A new genome-mining tool redefines the lasso peptide biosynthetic landscape

Tietz, J. I., Schwalen, C. J., Patel, P. S., Maxson, T., Blair, P. M., Tai, H. C., Zakai, U. I. & Mitchell, D. A., May 1 2017, In: Nature chemical biology. 13, 5, p. 470-478 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

AntiSMASH 4.0 - improvements in chemistry prediction and gene cluster boundary identification

Blin, K., Wolf, T., Chevrette, M. G., Lu, X., Schwalen, C. J., Kautsar, S. A., Suarez Duran, H. G., De Los Santos, E. L. C., Kim, H. U., Nave, M., Dickschat, J. S., Mitchell, D. A., Shelest, E., Breitling, R., Takano, E., Lee, S. Y., Weber, T. & Medema, M. H., Jul 3 2017, In: Nucleic acids research. 45, W1, p. W36-W41

Research output: Contribution to journal › Article › peer-review

Open Access

Chimeric Leader Peptides for the Generation of Non-Natural Hybrid RiPP Products

Burkhart, B. J., Kakkar, N., Hudson, G. A., Van Der Donk, W. A. & Mitchell, D. A., Jun 28 2017, In: ACS Central Science. 3, 6, p. 629-638 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Head-to-Head Prenyl Synthases in Pathogenic Bacteria

Schwalen, C. J., Feng, X., Liu, W., O-Dowd, B., Ko, T. P., Shin, C. J., Guo, R. T., Mitchell, D. A. & Oldfield, E., Jun 1 2017, In: ChemBioChem. 18, 11, p. 985-991 7 p.

Research output: Contribution to journal › Article › peer-review

Open Access

In Vitro Biosynthetic Studies of Bottromycin Expand the Enzymatic Capabilities of the YcaO Superfamily

Schwalen, C. J., Hudson, G. A., Kosol, S., Mahanta, N., Challis, G. L. & Mitchell, D. A., Dec 20 2017, In: Journal of the American Chemical Society. 139, 50, p. 18154-18157 4 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Mechanism of a Class C Radical S-Adenosyl- l -methionine Thiazole Methyl Transferase

Zhang, Z., Mahanta, N., Hudson, G. A., Mitchell, D. A. & Van Der Donk, W. A., Dec 27 2017, In: Journal of the American Chemical Society. 139, 51, p. 18623-18631 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Post-translational thioamidation of methyl-coenzyme M reductase, a key enzyme in methanogenic and methanotrophic archaea

Nayak, D. D., Mahanta, N., Mitchell, D. A. & Metcalf, W. W., Sep 7 2017, In: eLife. 6, e29218.

Research output: Contribution to journal › Article › peer-review

Open Access

Profiling of Microbial Colonies for High-Throughput Engineering of Multistep Enzymatic Reactions via Optically Guided Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Si, T., Li, B., Comi, T. J., Wu, Y., Hu, P., Wu, Y., Min, Y., Mitchell, D. A., Zhao, H. & Sweedler, J. V., Sep 13 2017, In: Journal of the American Chemical Society. 139, 36, p. 12466-12473 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Radical S-Adenosylmethionine Enzymes Involved in RiPP Biosynthesis

Mahanta, N., Hudson, G. A. & Mitchell, D. A., Oct 10 2017, In: Biochemistry. 56, 40, p. 5229-5244 16 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Reconstitution and Substrate Specificity of the Radical S-Adenosyl-methionine Thiazole C-Methyltransferase in Thiomuracin Biosynthesis

Mahanta, N., Zhang, Z., Hudson, G. A., Van Der Donk, W. A. & Mitchell, D. A., Mar 29 2017, In: Journal of the American Chemical Society. 139, 12, p. 4310-4313 4 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Structural insights into enzymatic [4+2] aza-cycloaddition in thiopeptide antibiotic biosynthesis

Cogan, D. P., Hudson, G. A., Zhang, Z., Pogorelov, T. V., Van Der Donk, W. A., Mitchell, D. A. & Nair, S. K., Dec 5 2017, In: Proceedings of the National Academy of Sciences of the United States of America. 114, 49, p. 12928-12933 6 p.

Research output: Contribution to journal › Article › peer-review

Open Access

2016

Biological characterization of the hygrobafilomycin antibiotic JBIR-100 and bioinformatic insights into the hygrolide family of natural products

Molloy, E. M., Tietz, J. I., Blair, P. M. & Mitchell, D. A., 2016, In: Bioorganic and Medicinal Chemistry. 24, 24, p. 6276-6290 15 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Biosynthetic Timing and Substrate Specificity for the Thiopeptide Thiomuracin

Zhang, Z., Hudson, G. A., Mahanta, N., Tietz, J. I., Van der Donk, W. A. & Mitchell, D. A., Dec 7 2016, In: Journal of the American Chemical Society. 138, 48, p. 15511-15514 4 p.

Research output: Contribution to journal › Article › peer-review

Open Access

In Vitro Biosynthesis and Substrate Tolerance of the Plantazolicin Family of Natural Products

Deane, C. D., Burkhart, B. J., Blair, P. M., Tietz, J. I., Lin, A. & Mitchell, D. A., Aug 19 2016, In: ACS chemical biology. 11, 8, p. 2232-2243 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Plantazolicin Is an Ultranarrow-Spectrum Antibiotic That Targets the Bacillus anthracis Membrane

Molohon, K. J., Blair, P. M., Park, S., Doroghazi, J. R., Maxson, T., Hershfield, J. R., Flatt, K. M., Schroeder, N. E., Ha, T. & Mitchell, D. A., Mar 11 2016, In: ACS Infectious Diseases. 2, 3, p. 207-220 14 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Targeting Reactive Carbonyls for Identifying Natural Products and Their Biosynthetic Origins

Maxson, T., Tietz, J. I., Hudson, G. A., Guo, X. R., Tai, H. C. & Mitchell, D. A., Nov 23 2016, In: Journal of the American Chemical Society. 138, 46, p. 15157-15166 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

2015

A prevalent peptide-binding domain guides ribosomal natural product biosynthesis

Burkhart, B. J., Hudson, G. A., Dunbar, K. L. & Mitchell, D. A., Aug 23 2015, In: Nature chemical biology. 11, 8, p. 564-570 7 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Douglas Alan Mitchell

Research output

Search results