Next-gen sequencing and metadata analyses of Great Lakes fungal data

  • Hannah E. Wahl (Creator)
  • Daniel B. Raudabaugh (Creator)
  • Elizabeth M. Bach (Creator)
  • Tiffany S. Bone (Creator)
  • Mark R. Luttenton (Creator)
  • R. H. Cichewicz (Creator)
  • Andrew Nicholas Miller (Creator)

Dataset

Description

The data set consists of Illumina sequences derived from 48 sediment samples, collected in 2015 from Lake Michigan and Lake Superior for the purpose of inventorying the fungal diversity in these two lakes. DNA was extracted from ca. 0.5g of sediment using the MoBio PowerSoil DNA isolation kits following the Earth Microbiome protocol. PCR was completed with the fungal primers ITS1F and fITS7 using the Fluidigm Access Array. The resulting amplicons were sequenced using the Illumina Hi-Seq2500 platform with rapid 2 x 250nt paired-end reads. The enclosed data sets contain the forward read files for both primers, both fixed-header index files, and the associated map files needed to be processed in QIIME. In addition, enclosed are two rarefied OTU files used to evaluate fungal diversity. All decimal latitude and decimal longitude coordinates of our collecting sites are also included.
Date made availableMar 1 2018
PublisherUniversity of Illinois at Urbana-Champaign

Keywords

  • Illumina
  • next-generation sequencing
  • ITS
  • fungi

Cite this

Wahl, H. E. (Creator), Raudabaugh, D. B. (Creator), Bach, E. M. (Creator), Bone, T. S. (Creator), Luttenton, M. R. (Creator), Cichewicz, R. H. (Creator), Miller, A. N. (Creator)(Mar 1 2018). Next-gen sequencing and metadata analyses of Great Lakes fungal data. University of Illinois at Urbana-Champaign. 10.13012/B2IDB-9320144_V2