Data for: Lipid heterogeneity between astrocytes and neurons revealed with single cell MALDI MS supervised by immunocytochemical classification

Dataset

Description

We have recently created an approach for high throughput single cell measurements using matrix assisted laser desorption / ionization mass spectrometry (MALDI MS) (J Am Soc Mass Spectrom. 2017, 28, 1919-1928. doi: 10.1007/s13361-017-1704-1. Chemphyschem. 2018, 19, 1180-1191. doi: 10.1002/cphc.201701364). While chemical detail is obtained on individual cells, it has not been possible to correlate the chemical information with canonical cell types.
Now we combine high-throughput single cell mass spectrometry with immunocytochemistry to determine lipid profiles of two known cell types, astrocytes and neurons from the rodent brain, with the work appearing as “Lipid heterogeneity between astrocytes and neurons revealed with single cell MALDI MS supervised by immunocytochemical classification” (DOI: 10.1002/anie.201812892).
Here we provide the data collected for this study. The dataset provides the raw data and script files for the rodent cerebral cells described in the manuscript.
Date made availableFeb 26 2019
PublisherUniversity of Illinois at Urbana-Champaign

Keywords

  • astrocyte
  • lipid analysis
  • mass spectrometry
  • Single cell analysis
  • neuron

Cite this

Neumann, E. (Creator), Comi, T. (Creator), Rubakhin, S. (Creator), Sweedler, J. V. (Creator) (Feb 26 2019). Data for: Lipid heterogeneity between astrocytes and neurons revealed with single cell MALDI MS supervised by immunocytochemical classification. University of Illinois at Urbana-Champaign. 10.13012/B2-3125702_V1